31,059 results
-
Plasmid#110804PurposeExpresses SaODH and opine dehydrogenase from Staphylococcus aureusDepositorAvailable SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only
-
L1-E309K
Plasmid#15487DepositorAvailable SinceOct. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYTK033
Plasmid#65140PurposeEncodes Venus as a Type 3 part to be used in the Dueber YTK systemDepositorInsertVenus
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB354
Plasmid#22069DepositorTypeEmpty backboneTagsT25ExpressionBacterialAvailable SinceOct. 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pET51b-His-TEV-HaloTag7_dead_mt
Plasmid#167267PurposeOverexpression of the dead variant (D106A) of HaloTag7 in E. coliDepositorInsertHaloTag7
TagsHis x10ExpressionBacterialMutationD106APromoterT7Available SinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
yqhD-RFP
Plasmid#63845PurposeThis plasmid is designed as a bacterial oxidative stress sensorDepositorInsertyqhD, mRFP1 and GFPmut3b
TagsmRFP1ExpressionBacterialMutationGene is translationally fused to RFPAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2H12Matry-blind
Plasmid#221181PurposecdGreen-Matry(blind) expressed from an aTc-inducible promoter (Internal Lab ID: UJ11209)DepositorInsertcdGreen-Matry(blind)
ExpressionBacterialPromoterPtetAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-NHSTIIT-AVITAG-GG
Plasmid#215802PurposeCloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAGDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHIT179
Plasmid#204510PurposeBacterial expression of CSR-1a with 6xHis and maltose binding protein tags for in vitro assaysDepositorAvailable SinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
BASIC_SEVA_66.11
Plasmid#194213PurposeBASIC DNA assembly vectorDepositorInsertmScarlet counter-selection cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 26, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHH0103_NR4A3_Hormone-recep
Plasmid#109952PurposeProtein expression and purification of NR4A3_Hormone-recepDepositorInsertNR4A3_Hormone-recep
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_RORB_Hormone-recep
Plasmid#109958PurposeProtein expression and purification of RORB_Hormone-recepDepositorInsertRORB_Hormone-recep
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_HNF4G_Hormone-recep
Plasmid#109916PurposeProtein expression and purification of HNF4G_Hormone-recepDepositorInsertHNF4G_Hormone-recep (HNF4G Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
AND-gate-V2.0
Plasmid#44449DepositorInsertAND-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEB2-TurboRFP
Plasmid#103996PurposePlasmid encoding TurboRFPDepositorInsertTurboRFP
UseLow copyExpressionBacterialMutationMutations relative to eqFP578 (R32G, T68A, L79F, …PromoterproCAvailable SinceDec. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
SEPTOPO
Plasmid#64944Purposesource of SEP (Super Ecliptic pHluorin) sequenceDepositorInsertSEP
ExpressionBacterialPromoterlacAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dapp1-PH pET11a
Plasmid#71376PurposePhosphoinositide BindingDepositorInsertDapp1-PH (DAPP1 Human)
ExpressionBacterialMutationPleckstrin homology (PH) domain; aa 148-273PromoterT7Available SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
EPHB1_HUMAN_D0
Plasmid#79694PurposeThis plasmid encodes the kinase domain of EPHB1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable SinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-human MIZ1 wild type
Plasmid#74161Purposebacterial expression of MIZ1DepositorAvailable SinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L TriCatcher-RGDSP
Plasmid#112631PurposeTwo SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcherDepositorInsertTriCatcher-RGDSP
TagsHis6 and TEV cleavage siteExpressionBacterialMutationcentral RGDSP integrin-binding sequencePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046806
Plasmid#145746PurposeBacterial Expression plasmid for SARS-CoV-2 M (membrane)DepositorInsertSARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC19-T7-Bottom-T
Plasmid#87310PurposeExpresses the "Bottom" transcript of the Split-Broccoli system under the control of the T7 promoter.DepositorInsertSplit-Broccoli system "Bottom" transcript
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILxR5
Plasmid#149497PurposeBroad-Host range expression vector, pUC origin, Tn7 recognition sites, LuxR activator, PLuxB promoter, mRFP, Gentamicin Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOGG216
Plasmid#114002PurposeLevel 2 golden gate cloning vector for medium copy number free-living expressionDepositorTypeEmpty backboneExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBetI-E1
Plasmid#74677PurposeBetI-E1 gateDepositorInsertPTac-RBSE1-BetI-PBetI-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
Gma-pCAMBIA-1301-STTM171
Plasmid#84080PurposeDisturb the expression of miR171 in soybeanDepositorInsertmiR171
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCF525-EF1a-Hygro-P2A-AcrVA4-lenti
Plasmid#115663PurposeExpresses AcrVA4 in human cells, contained in a lentiviral vector.DepositorInsertAcrVA4
UseLentiviralExpressionBacterial and MammalianPromoterEF-1a promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pELlR1
Plasmid#172569PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pELlR2
Plasmid#172555PurposeInducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertLacI-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLlacO-1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
Plasmid#101211PurposeBacterial expression plasmid for SpCas9-HF1, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, N497A, R661A, Q695A and…PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
Plasmid#101204PurposeBacterial expression plasmid for SpCas9∆REC3, REC2 FRET variantDepositorInsertSpCas9 variant C80S/C574S/E60C/D273C/M1–N497,GGS,V713–D1368
Tags10x His, MBP, and TEV siteExpressionBacterialMutationC80S, C574S, E60C, D273C, M1-N497, GGS, V713-D1368PromoterT7Available SinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 FlAsH LIC cloning vector (H6-FlAsH)
Plasmid#30184DepositorTypeEmpty backboneTags6xHis, AviTag (biotin), FlAsH, and TEV cleavage s…ExpressionBacterialAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
TagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable SinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcrRNA.con
Plasmid#61285PurposeConstitutive repeat-only E. coli CRISPR arrayDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterJ23119Available SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorAvailable SinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNP1 tdTomato toehold switch
Plasmid#107354PurposeExpresses tdTomato (a DsRed derivative) in Ecoli off a T7 promoter when pNP1 tdTomato trigger is co-expressedDepositorInserttoehold switch 5 + tdTomato
ExpressionBacterialPromoterT7Available SinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB061i2
Plasmid#79229PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4 (Y87A); PlacIq lacI
ExpressionBacterialPromoterPpro1; PlacIqAvailable SinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHH29_32 series plasmid
Plasmid#120901PurposeAlso known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated deviceDepositorInsertsEc-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor
UseSynthetic BiologyExpressionBacterialAvailable SinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-156
Plasmid#118538PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmCardinal
UseMinitn5 delivery vectorExpressionBacterialAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only