32,157 results
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Plasmid#197046PurposeHis6 tagged Atg24 from S. cerevisiae for expression in E. coli.DepositorInsertAtg24
Tags6His-TEVExpressionBacterialAvailable SinceJuly 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET24a HAB1 (172-511)
Plasmid#177844PurposeBacterial Expression of HAB1DepositorInsertProtein phosphatase 2C 16
Tags6xHis-GSTExpressionBacterialAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
gNS2B47NS3 L30SF31S
Plasmid#155316PurposeDENV4 47 peptides from NS2B cofactor linked to NS3 full length by GGGGSGGGG linker L30SF31S mutantDepositorInsertDENV4 NS3 full length protein linked with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationL30 to S, F31 to SPromoterM13Available SinceSept. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
RAB14
Plasmid#25519PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceJan. 11, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
H6SUMO-IntC-P53(304-393)
Plasmid#200316Purposebacterial expression of P53 TET-CTD for segmental labelingDepositorInsertp53 (304-393) (TP53 Human)
TagsHis6-SUMO and designed intein CExpressionBacterialPromoterT7Available SinceMay 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(1-303)IntN
Plasmid#200312PurposeBacterial expression of P53 NTAD-DBD for segmental labelingDepositorInsertp53 (TP53 Human)
TagsHis6GB1tev and designed intein NExpressionBacterialMutationM133L, V203A, N239Y, N268DPromoterT7Available SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
GST-MAGE-2-429
Plasmid#89129Purposebacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sitesDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-M-G6
Plasmid#73437PurposeCRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.DepositorInsertRepressor G6 (orthogonal T7-lac repressor)
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterConstitutive wild-type S. pyogenes promoterAvailable SinceMarch 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046833
Plasmid#145649PurposeBacterial Expression plasmid for SARS-CoV-2 nsp9DepositorInsertSARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJK_proK1_eGFP
Plasmid#59853PurposeConstitutively expresses eGFP with proK1 promoterDepositorInsertEnhanced Green Fluorescent Protein
Tags6x-HisExpressionBacterialMutationS2R,S65G,S72APromoterproK1Available SinceOct. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCas_5+7_gfp
Plasmid#60595Purposeencodes a reversed Int7 gene flanked by the attB/P sites of Int5, as well as a reversed GFP (GFPmut33) gene flanked by the attB/P sites of Int7.DepositorInsertCas_5+7_gfp
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCas_2+5
Plasmid#60594Purposeencodes the Int2 gene controlled by PBad promoter and a reversed Int5 gene flanked by the attB/P sites of Int2DepositorInsertCas_2+5
ExpressionBacterialAvailable SinceApril 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKB230
Plasmid#170017PurposeEncodes LacI-controlled expression of surface-displayed protegrin-1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Protegrin-1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGBK CUP1 EGFP
Plasmid#170210PurposeYeast two-hybrid vector using a copper inducible CUP1 promoter, expressing EGFPDepositorArticleInsertenhanced green fluorescent protein
TagsGAL4 DNA Binding Domain and MycExpressionBacterial and YeastPromoterCUP1Available SinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
p3.5VPIII.EGFP.IGR2.1
Plasmid#40865DepositorAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-cyScarlet
Plasmid#191260PurposeBacterial expression plasmids for Long Stokes shift red fluorescent protein cyScarletDepositorInsertcyScarlet
TagsHis tagExpressionBacterialAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pET19-MTdTc302
Plasmid#207458PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT) cysteine variant. Only one surface-exposed cysteine, at residue 302DepositorInsertMTdTc302 (Dntt Mouse)
Tags10xHis-MBPExpressionBacterialMutationCys188Ala, Cys216Ser, Cys378Ala, and Cys438SerAvailable SinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
XNOR-gate-V2.0
Plasmid#44454DepositorInsertXNOR-gate
UseSynthetic BiologyExpressionBacterialAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETx-SR6c3
Plasmid#176211PurposeBacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike proteinDepositorInsertSR6c3
Tags6XHisExpressionBacterialPromoterT7Available SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBS mEya1 (CT#671)
Plasmid#13971DepositorInsertEya1 (Eya1 Mouse)
ExpressionBacterialAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCH6 - pBait-ChiX
Plasmid#174662Purposearabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.DepositorInsertChiX
Tags1xMS2 hairpinExpressionBacterialPromoterpBadAvailable SinceMay 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-mTDGa.0
Plasmid#81050Purposebacterial expression of murine TDGDepositorAvailable SinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
SBC015866
Plasmid#226280PurposeExpresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.DepositorInserts4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase
ExpressionBacterialAvailable SinceNov. 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pQTEV-PEPD
Plasmid#31328DepositorAvailable SinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12DKR-J
Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23-PBD(65-150)-N-Cys
Plasmid#13722DepositorInsertPBD(65-150)-N-Cys (PAK1 Human)
Tags6xHisExpressionBacterialMutationMet-Cys-Gly-PBD(65-150)-Available SinceMarch 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pET-51b(+) ChemoG5
Plasmid#193805PurposeProtein production of ChemoG5 in bacteriaDepositorInsertChemoG5
Tags10xHisExpressionBacterialMutationEGFP:A206K, T225R; HT7: E143R, E147R, L271EPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
KTK_354
Plasmid#180525PurposeKTK compatible Intergration/suicide plasmid. Contains a LacZ dropout cassette flanked by regions homolgous to the arsenic resistance cassete.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
Reb_140 aka PAS625
Plasmid#71230PurposeReb_1 with RebA(S88P)DepositorInsertReb locus
TagsnoneExpressionBacterialMutationRebA(S88P)Available SinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMCSG53_Nsp15
Plasmid#167265PurposeBacterial expression of Nsp15DepositorInsertNsp15 (residues 1-346) (ORF1ab SARS-CoV-2)
TagsHis6-TEV siteExpressionBacterialMutationnoneAvailable SinceMay 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PdnaK-IR3-IR3-Bxb1-CadR-pZa
Plasmid#204686PurposeCadmium responsive genetic circuitDepositorInsertBxb1 (Bxb1p45 Synthetic)
UseSynthetic BiologyTagsCadRExpressionBacterialPromoterInducible, HspR is its repressorAvailable SinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWarf(+)
Plasmid#34562DepositorInsertHRV 3C-GPA-GFP-8HIS
ExpressionBacterialAvailable SinceFeb. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
130_pSKB3-LUMP-P.leioghnati
Plasmid#65894PurposeBacterial expression of P. leioghnati LUMP protein, a FRET acceptor protein emitting a cyan-colored fluorescence with an unusually long excited state lifetimeDepositorInsertLUMP
ExpressionBacterialAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP -R
Plasmid#48887PurposePositive feedback plasmid from oscillator without luxRDepositorInsertsfGFP and luxI
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialPromoterpLuxAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4 WW domain #1
Plasmid#104208PurposeBacterial expression of WW domain #1 from NEDD4DepositorInsertNEDD4 WW domain #1 (NEDD4 Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceFeb. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLitR-L1
Plasmid#74683PurposeLitR-L1 gateDepositorInsertPTac-RBSL1-LitR-PLitR-YFP
ExpressionBacterialAvailable SinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only