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32,812 results

Showing: 7741 - 7770 of 32812 results

  3. pET28-Nsp14-TEV-Strep

    Plasmid
    #154953
    Purpose
    Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli
    Depositor
    Justin Kollman
    Insert
    Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
    Tags
    TEV-Strep
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154953-plasmid-map-sequence-id-307852

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  5. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101 and VanCC
    Available Since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

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  8. pET-HIS-Sangamo

    Plasmid
    #40786
    Purpose
    for e. coli expression, Golden Gate Compatible TALEN incorporated with N Terminal 6xHIS Tag, Codon Optimized FokI
    Depositor
    Gang Bao
    Insert
    Transcription Activator Like Effector Nuclease
    Use
    TALEN
    Tags
    N Terminal 6xHIS
    Expression
    Bacterial
    Mutation
    +63 C Terminus, Codon Optimized FokI Endonuclease
    Promoter
    T7
    Available Since
    Dec. 12, 2013
    Availability
    Industry, Academic Institutions, and Nonprofits
    40786-plasmid-map-sequence-id-68901

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  9. pRL542

    Plasmid
    #70282
    Purpose
    ColK-based vector; provides a nicking function to permit conjugal transfer and bears a polylinker for insertion of methylase-encoding genes
    Depositor
    Peter Wolk
    Article
    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Feb. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70282-plasmid-map-sequence-id-485424

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  16. pTCF10 HLA-B*08:01

    Plasmid
    #180447
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*08:01
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180447-plasmid-map-sequence-id-355840

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  18. pHL 1405

    Plasmid
    #53010
    Purpose
    pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    DsrA sRNA
    hfq
    sodB
    Use
    Synthetic Biology
    Tags
    gfp
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  23. pRPCN

    Plasmid
    #193726
    Purpose
    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193726-plasmid-map-sequence-id-381907

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  27. pAH121

    Plasmid
    #164901
    Purpose
    The pAH121 plasmid [Haldimann and Wanner 2001] is the phi21 helper plasmid necessary for genomic integration of the pCYMR expression plasmid.
    Depositor
    Keith Shearwin
    Article
    Hao et al Nucleic Acids Res. 2021 Jan 28. pii: 612
    Insert
    phi21 Integrase
    Expression
    Bacterial
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    164901-plasmid-map-sequence-id-327647

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Showing: 7741 - 7770 of 32812 results