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32,167 results

Showing: 7741 - 7770 of 32167 results

  6. pSBY3_ligD

    Plasmid
    #104624
    Purpose
    CRISPR system used to genome editing in Mycobacterium smegmatis, expresses Fn Cpf1 and crRNA targeting ligD
    Depositor
    Sheng Yang
    Article
    Sun et al Biotechnol J. 2018 Sep;13(9):e1700588. d
    Insert
    FnCpf1
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    FnCpf1 codon-optimized C.glutamicum
    Promoter
    hsp60
    Available Since
    June 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104624-plasmid-map-sequence-id-201663

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  14. Ec dnaN-pHK/P

    Plasmid
    #237239
    Purpose
    over-express E. coli hk/P-dnaN (Pol III beta sliding clamp subunit, N-terminal cleveable his-kinase tag)
    Depositor
    Michael O'Donnell
    Insert
    dnaN (beta), N-terminal his-kinase cleavable tag (dnaN E. coli)
    Tags
    his-10+PKA kinase motif+PreScission Protease clea…
    Expression
    Bacterial
    Available Since
    April 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    237239-plasmid-map-sequence-id-469256

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  18. BCL2A1A

    Plasmid
    #42399
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BCL2A1A (BCL2A1 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pGM190oriT

    Plasmid
    #115679
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    oriT, aphII, tsr
    Expression
    Bacterial
    Promoter
    tipA
    Available Since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    115679-plasmid-map-sequence-id-410983

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  21. pHN872

    Plasmid
    #178830
    Purpose
    Bacterial expression for human Saposin A
    Depositor
    Kazuhiro Abe
    Insert
    artificial gene encoding human Saposin A
    Tags
    GST, His8, TEV
    Expression
    Bacterial
    Mutation
    Codon-usage was optimized for bacterial expressio…
    Promoter
    T7
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178830-plasmid-map-sequence-id-351931

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  23. pSUB7

    Plasmid
    #169698
    Purpose
    Template plasmid for generating C-terminal 6-His epitope tag fusions to bacterial genes by "Lambda red" recombineering.
    Depositor
    Nara Figueroa
    Article
    Uzzau et al Proc Natl Acad Sci U S A. 2001 Dec 18;98
    Insert
    6xHis epitope tag adjacent to FRT-flanked aph (KanR) gene
    Tags
    6xHis tag
    Expression
    Bacterial
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169698-plasmid-map-sequence-id-334547

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  25. pBFC0625

    Plasmid
    #186691
    Purpose
    VcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargo
    Depositor
    Jennifer Doudna
    Article
    Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:
    Inserts
    tnsA
    tnsB
    tnsC
    tnsD
    tniQ
    cas8-cas5 fusion
    cas7
    cas6
    Vc_lacZ_α_1 Spacer crRNA
    Tn7 transposon
    GmR+sfGFP VcDART Cargo
    Expression
    Bacterial
    Promoter
    PLac
    Available Since
    Sept. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    186691-plasmid-map-sequence-id-371386

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  26. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid
    #90473
    Purpose
    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine
    Depositor
    Hermann Gaub
    Insert
    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    Tags
    Cysteine and Sortase-Tag
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    90473-plasmid-map-sequence-id-191118

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  29. pTXBI-mclover-YTHDC1-FL

    Plasmid
    #177121
    Purpose
    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Article
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177121-plasmid-map-sequence-id-348688

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  30. pGWB508-cTP-sfGFP

    Plasmid
    #213863
    Purpose
    Binary vector for the expression of sfGFP flanked with an N-terminal plastid transit peptide in plants (for Agrobacterium-mediated genetic transformation and visualization of plastid.)
    Depositor
    Yutaka Kodama
    Article
    Ishikawa et al Plant Cell Rep. 2023 Mar;42(3):599-607.
    Insert
    Plastid transit peptide fused sfGFP
    Expression
    Bacterial and Plant
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    213863-plasmid-map-sequence-id-425331

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Showing: 7741 - 7770 of 32167 results