32,167 results
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Plasmid#193802PurposeProtein production of ChemoG3.1 in bacteriaDepositorInsertChemoG 3.1
Tags10xHisExpressionBacterialMutationEGFP: A206K, T225RPromoterT7Available SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCPP5912
Plasmid#128703PurposePhytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor cloneDepositorInsertshcE-avrE1
ExpressionBacterialMutationThree synonymous mutations Ala 362 (GCT-GCC), Ala…Available SinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
ETV5_pet28a
Plasmid#131639PurposeBacterial expression of ETS gene ETV5DepositorAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMLM36 (aka pBR-UV5-GP-FD2)
Plasmid#21871DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pEM8317
Plasmid#213989PurposePrpsM driven expression of sfGFPDepositorInsertsfGFP
ExpressionBacterialPromoterPrpsMAvailable SinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDule-3-nitroTyrosine (A7)
Plasmid#174078PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70T D158H I159A L162RPromoterGlnRS (constitutive)Available SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKB231
Plasmid#170018PurposeEncodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporterDepositorArticleInsertsmNeonGreen
Cecropin P1
UseSynthetic BiologyTagsLpp, OmpA, and TetherExpressionBacterialPromoterPtac and PvirKAvailable SinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS2a-RFP
Plasmid#35328DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pNM262
Plasmid#128412PurposeVanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.DepositorInsertLysostaphin (epr Staphylococcus simulans)
UseSynthetic BiologyTagsamyE SPExpressionBacterialPromoterPspank(V)Available SinceSept. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 ProteinG TEV LIC cloning vector (2P-T)
Plasmid#29713DepositorTypeEmpty backboneTagsHis6-ProteinG-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-2xMreBN-mCherry-His
Plasmid#133626PurposeExpression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.DepositorInsert2xMreB-mCherry-His
TagsHisExpressionBacterialAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pV1F(Spec)
Plasmid#205086PurposeProkaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.DepositorInsertAtVIP1
ExpressionBacterialAvailable SinceSept. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
YWHAQ
Plasmid#38931PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
APneg-VAMP1b
Plasmid#168008Purposenegative selection PACE Accessory PlasmidDepositorInsertVAMP1(T29-K89)-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
APneg-VAMP1c
Plasmid#168009Purposenegative selection PACE Accessory PlasmidDepositorInsertVAMP1(T29-K89)-T7PAP
ExpressionBacterialAvailable SinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Ki67 (8 repeats+LR)-mNeonGreen-IRESpuro2
Plasmid#183741PurposeExpresses Ki67 (8 repeats+LR)-mNeonGreenDepositorInsertMKI67 LR domain + 8 Ki-67 repeats (MKI67 Human)
TagsmNeonGreenExpressionBacterial and MammalianMutationMKI67 LR domain + 8 Ki-67 repeatsPromoterCMVAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15p65
Plasmid#27661DepositorInsertTetrahymena telomerase holoenzyme p65
TagsHisExpressionBacterialAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMUT2-VtrA-mCherry
Plasmid#192862PurposepMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917DepositorInsertVtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
ExpressionBacterialAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
BASIC_8_RBS34- mCherry -B0015
Plasmid#68142Purposestorage vector for BASIC part: RBS-RFP(ORF)DepositorInsertRBS34-mcherry-B0015
ExpressionBacterialAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
His_Naa80d in pTYB12
Plasmid#188455PurposeExpress in E. coli human Naa80 delta (actin's N-terminal acetyl transferase) with N-terminal intein-chitin binding domain tag and 6xHis tag.DepositorAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-81nmt1
Plasmid#105164PurposeYeast gene targetingDepositorInserthphMX6-81nmt1
Tags81nmt1ExpressionBacterial and YeastPromoter81nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-41nmt1
Plasmid#105163PurposeYeast gene targetingDepositorInserthphMX6-41nmt1
Tags41nmt1ExpressionBacterial and YeastPromoter41nmt1Available SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
Tus pGST-//
Plasmid#53305PurposeExpresses GST-tagged E.coli replication terminator protein Tus.DepositorInsertE.Coli Tus replication terminator protein
TagsGSTExpressionBacterialAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only