32,812 results
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Plasmid#69109PurposeFor bacterial expression of recombinant human GTSE1 tagged with MBP and His.DepositorAvailable SinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only
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pBALU1
Plasmid#69316PurposeEncodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of gfpDepositorInsertGFPint-FgF
ExpressionBacterialAvailable SinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP S2TAG
Plasmid#68296PurposeS2TAG GFP reporterDepositorInsertGFP S2TAG
TagsHisExpressionBacterialAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1 S222TAG
Plasmid#68302PurposeN-terminal MBP tagged human MEK1 gene S222TAG for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_4_Cm
Plasmid#68138Purposestorage vector for BASIC part: CmDepositorInsertChloramphenicol
ExpressionBacterialAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST50Trc1-HISDHFR
Plasmid#63935PurposePosition 1 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive controlDepositorTypeEmpty backboneTags6xHisExpressionBacterialPromoterT7Available SinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
BASIC_3_Kan
Plasmid#68137Purposestorage vector for BASIC part: KanDepositorInsertKanamycin
ExpressionBacterialAvailable SinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBW414hrpL-cIgfp
Plasmid#61439PurposepSB4A3 harbouring hrpL-rbs34-cI-lva-ter-Plam-rbs30-gfp-terDepositorInsertHrpL-rbs34-CI-LVA-t-plam-30gfp-t
TagsLVAExpressionBacterialPromoterHrpLAvailable SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC210
Plasmid#65950PurposeWeak Repressor plasmid for two-strain systemDepositorInsertCinI
TagsLAA taggedExpressionBacterialPromoterPrhl/lac-wAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pC165
Plasmid#65949PurposeP1 activator plasmid for two-strain systemDepositorAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - CA
Plasmid#65424PurposeRhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63LDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, Q63L in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
RhoC FLARE.sc mCer, mVenus - DN
Plasmid#65423PurposeRhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19NDepositorInsertRBD, mCerulean, mVenus, RhoC
ExpressionBacterial, Insect, and Mamm…MutationT153M in mVenus, T19N in RhoCAvailable SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB3-Flag PLK1 T210D
Plasmid#68272Purposebacterial expression of Plk1 T210D mutantDepositorInsertPLK1 (PLK1 Human)
TagsFlag and Mxe intein/chitin binding domainExpressionBacterialMutationT210DPromoterT7Available SinceAug. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
274_pSKB3-Venus-LUMP
Plasmid#65976PurposeBacterial expression of Venus-LUMP fusion with thrombin cleavage site, a FRET-based thrombin sensorDepositorInsertLUMP
TagsVenus and thrombin site (11-residue linker that h…ExpressionBacterialPromoterT7Available SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
Fast-GCaMP6f-RS06
Plasmid#67159Purposegenetically encodable fluorescent calcium indicator protein to monitor neuronal activityDepositorInsertFast-GCaMP6f-RS06
Tags6xHis, T7 tag, and Xpress tagExpressionBacterialMutationM374Q within GCaMP6fPromoterT7Available SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET3a hRegIIIa
Plasmid#64937Purposebacterial expression of processed human RegIIIalphaDepositorInsertRegIIIa (REG3A Human)
ExpressionBacterialMutationprocessed form. mutations made in 5' end to …PromoterT7Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-H10-mCherry
Plasmid#66531PurposeExpresses mCherry under the control of Mutant T7 Promoter H10DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - H10Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-mCherry
Plasmid#66533PurposeExpresses mCherry under the control of Mutant T7 Promoter G6DepositorInsertmCherry
UseSynthetic BiologyExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - G6Available SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK077
Plasmid#65184PurposeEncodes KanamycinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertKanamycinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK080
Plasmid#65187PurposeEncodes ZeocinR as a Type 6 part to be used in the Dueber YTK systemDepositorInsertZeocinR
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK069
Plasmid#65176PurposeEncodes ConR3 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR3
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK036
Plasmid#65143PurposeEncodes Cas9 as a Type 3 part to be used in the Dueber YTK systemDepositorInsertCas9
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK017
Plasmid#65124PurposeEncodes pRPL18B as a Type 2 part to be used in the Dueber YTK systemDepositorInsertpRPL18B
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYTK004
Plasmid#65111PurposeEncodes ConL2 as a Type 1 part to be used in the Dueber YTK systemDepositorInsertConL2
ExpressionBacterialAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pASKt15C+SrtA7woH
Plasmid#65020PurposeExpresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coliDepositorInsertsortase A
TagsS tagExpressionBacterialMutationdeleted amino acids 1-59, P94R, E105K, E108A, D16…Promotertetracycline promoter/operatorAvailable SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET21a-Med25-VBD-6His
Plasmid#64773PurposeBacterial expression of His-tagged Med25-VBDDepositorAvailable SinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG 627
Plasmid#63872PurposeMeasurements of translational accuracyDepositorAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG 12-6
Plasmid#63870PurposeMeasurements of translational accuracyDepositorAvailable SinceApril 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAND_Reporter
Plasmid#60591PurposeAND gate reporterDepositorInsertAND_Reporter
ExpressionBacterialAvailable SinceApril 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(H66)
Plasmid#63558PurposeExpression of the Azure (blue) spectral variant of eGFP in bacteria and in mammalian cells. Could be used as a reporter for quantifying gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(H66)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged tyrosine at position 66 with respect to t…PromoterCMV-EF1α hybrid (CEF)Available SinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only