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32,161 results

Showing: 11161 - 11200 of 32161 results

  3. HisMS2_VLP_FluA

    Plasmid
    #175955
    Purpose
    Generation of MS2 Virus-Like Particles packaged with the sequence for the Influenza A Matrix Protein [A/Illinois/20/2018(H1N1)]
    Depositor
    Paul Freemont
    Article
    Unruh et al medRxiv 2024.04.17.24305965
    Insert
    M gene
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 19, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    175955-plasmid-map-sequence-id-347829

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  4. N-BLa 1.1

    Plasmid
    #88994
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.1 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    88994-plasmid-map-sequence-id-177496

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  5. MSP2279

    Plasmid
    #70706
    Purpose
    Bacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus dCas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    D10A and H557A in SaCas9
    Promoter
    T7
    Available Since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70706-plasmid-map-sequence-id-127768

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  7. pET26b(+)-ATI_QconCAT_1

    Plasmid
    #163957
    Purpose
    Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.
    Depositor
    Stefan Tenzer
    Article
    Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102
    Insert
    ATI QconCAT
    Tags
    polyhistidine tag
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    163957-plasmid-map-sequence-id-321834

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  11. TBXTA-c043

    Plasmid
    #139766
    Purpose
    Protein expression/Streptavidin pull-down
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    Avi-tag (Biotin) and His6-TEV
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41- D225
    Promoter
    T7
    Available Since
    May 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    139766-plasmid-map-sequence-id-296581

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  13. pxCas9CR4

    Plasmid
    #111656
    Purpose
    PCas9CR4 with the xCas9 mutations allowing NG PAM sites
    Depositor
    Christopher Reisch
    Insert
    TetR and xCas9
    Use
    Synthetic Biology
    Tags
    ssrA
    Expression
    Bacterial
    Mutation
    xCas9 mutations
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111656-plasmid-map-sequence-id-217189

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  15. pUS252b

    Plasmid
    #191831
    Purpose
    Expresses fuGFPb constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuGFPb to place in other plasmid backbones
    Depositor
    Nicholas Coleman, Mark Somerville
    Insert
    fuGFPb
    Expression
    Bacterial
    Available Since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191831-plasmid-map-sequence-id-382128

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  16. pAV106

    Plasmid
    #63196
    Purpose
    pRS406 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Expression
    Bacterial and Yeast
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    63196-plasmid-map-sequence-id-104896

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  19. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  22. TPMT

    Plasmid
    #25497
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TPMT (TPMT Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. pTU2-a_Sv1KA_amilCP

    Plasmid
    #204054
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var1 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204054-plasmid-map-sequence-id-418176

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  27. pTU2-a_Sv2KA_amilCP

    Plasmid
    #204053
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101-var2 ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    204053-plasmid-map-sequence-id-418175

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  28. pTU2-a_SKA_amilCP

    Plasmid
    #204055
    Purpose
    Type TU2-a backbone with kanamycin resistance, pSC101 (WT) ORI, and Axe-Txe plasmid stability cassette. amilCP dropout vector.
    Depositor
    Mikhail Shapiro
    Type
    Empty backbone
    Expression
    Bacterial
    Mutation
    None
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    204055-plasmid-map-sequence-id-413881

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  34. pDTFendoNA2

    Plasmid
    #174731
    Purpose
    Expresses DT386F-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a furin-cleavable linker.
    Depositor
    Jukka Finne
    Article
    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-
    Insert
    DT386F
    Expression
    Bacterial
    Mutation
    Deleted amino acids 412-560 (the receptor binding…
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    174731-plasmid-map-sequence-id-343522

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  38. MP6.6TAG_AraC_Para-dnaQ926-dam-seqA-emrR-ugi-CDA1.6TAG

    Plasmid
    #163719
    Purpose
    Arabinose-inducible expression of six mutagenesis genes, each encoding one amber codon after the initiator methionine
    Depositor
    Matthew Bennett
    Article
    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25
    Insert
    araC dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TAG CDA1.1TAG
    Expression
    Bacterial
    Mutation
    dnaQ926.1TAG dam.1TAG seqA.1TAG emrR.1TAG ugi.1TA…
    Available Since
    Sept. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163719-plasmid-map-sequence-id-323479

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Showing: 11161 - 11200 of 32161 results