32,167 results
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Plasmid#74647PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianPromoter-Available SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJMP1217
Plasmid#119258Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
UBQLN2_450-624W
Plasmid#176852PurposeExpresses human UBQLN2 construct 450-624 with added C-terminal W in bacterial cellsDepositorInsertUBQLN2_450C_W (UBQLN2 Human)
ExpressionBacterialMutationdeletion of residues 1-449 and addition of C-term…PromoterT7Available SinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT21
Plasmid#200995PurposepLv2-pUC18R6KT-miniTn7-Gm, GmR, ApRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT23
Plasmid#200997PurposepLv2-pUC18R6KT-miniTn7-Tc, TcR, ApRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT24
Plasmid#200998PurposepLv2-pUC18R6KT-miniTn7-aad9, SpR, StR, ApRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT14.Y
Plasmid#201009PurposepLv1-pUC18R6KT-miniTn7-aad9-sYFP2, SpR,StR, ApRDepositorInsertsYFP2
ExpressionBacterialPromoterJ23100Available SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTn7-SCOUT20
Plasmid#200990PurposepL0V-Lv2-pUC18R6KT-miniTn7-GG-Esp3I sites for Antb modules, ApRDepositorTypeEmpty backboneExpressionBacterialAvailable SinceMay 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CRK-1/2
Plasmid#91367PurposeProtein expression and purification of human SH3 domain construct CRK-1/2DepositorInsertCRK-1/2 (CRK Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T SHP1 CS
Plasmid#8595DepositorAvailable SinceJune 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTCF3 H-2D(d)
Plasmid#180452PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2D(d)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available SinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA221TrxA-AID-T7RNAP
Plasmid#167976PurposeExpresses fusion protein TrxA-AID-T7RNAP in E. coliDepositorInsertTrxA-AID-T7RNAP
TagsT7RNAP and TrxAExpressionBacterialPromoterPtetAvailable SinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP1069
Plasmid#119244Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
ExpressionBacterialMutationD10A and H840AAvailable SinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7Available SinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRD421
Plasmid#163112PurposeProduction of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysSDepositorInsertHis-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
TagsHis-tag with TEV-cleavable linkerExpressionBacterialPromoterT7 promoterAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
SMYD3
Plasmid#25318PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGST TCF4 DBD
Plasmid#16488DepositorAvailable SinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET21c-His-Xa-Gluc-Xa-C9D-TGinsert
Plasmid#124660Purposereporter protein which emits bright blue fluorescenceDepositorInsertGaussia luciferase-C9D-TGinsert
TagsIEGRTMGDDDGDDDGDDD(FactorXa,TMG,C9D-Tag) and MHHH…ExpressionBacterialMutationchanged glutamic acid 100 to alanine, and glycine…PromoterT7 promoterAvailable SinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lbu_C2c2_E299A
Plasmid#91855PurposeBacterial expression for Cas13aDepositorInsertLbuCas13a (LbuC2c2) E299A
TagsHis6-MBP-TEV siteExpressionBacterialMutationE299AAvailable SinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETC-RdgBbeta-splice variant 1
Plasmid#58276Purposebacterial expression of human RdgBbeta splice variant 1DepositorAvailable SinceAug. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986H
Plasmid#174801PurposeBacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overallDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-Y986S
Plasmid#174802PurposeBacterial expression of a PARP1 mutant that produces mainly short PAR oligomersDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G972R
Plasmid#174800PurposeBacterial expression of a PARP1 mutant that produces less PAR oligomers overallDepositorAvailable SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV C
Plasmid#145359PurposeCell-free/mammalian expression vector for Zika virus capsid protein with N-term mCherryDepositorInsertZIKV Capsid
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-5/5
Plasmid#91464PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-5/5DepositorInsertSH3PXD2A-5/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-1/5
Plasmid#91351PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-1/5DepositorInsertSH3PXD2A-1/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3PXD2A-3/5
Plasmid#91352PurposeProtein expression and purification of human SH3 domain construct SH3PXD2A-3/5DepositorInsertSH3PXD2A-3/5 (SH3PXD2A Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGR-ECK120033127
Plasmid#46011DepositorInsertECK120033127 Terminator
UseSynthetic BiologyExpressionBacterialPromoterPBADAvailable SinceJune 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046810
Plasmid#149251PurposeBacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)DepositorInsertSARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pTn7·xylS
Plasmid#122591PurposePlasmid for genomic integraction of the xylS regulator into the Tn7 insertion siteDepositorInsertxylS
UseSynthetic Biology; Tn7 genomic integrationExpressionBacterialPromoterPmAvailable SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetOminiCMV-SM
Plasmid#136578PurposeDox-inducible polycistronic lentiviral vector expressing mouse Sox2, cMycDepositorUseLentiviralExpressionBacterialPromotertetO-miniCMV (dox-inducible)Available SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET15-MTFmt
Plasmid#31174DepositorInsertMature form bovine mitochondrial methionyl-tRNA transformylase (MTFMT Bovine)
TagsHis and thrombin cleavageExpressionBacterialAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAR3040-spitz
Plasmid#38341DepositorInsertspitz (spi Fly)
ExpressionBacterialAvailable SinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTE1059
Plasmid#186628PurposeExpression of S. coelicolor butyrolactone biosynthesis genes in E. coli. Heterologous production of SCBs.DepositorInsertsscbA
scbB
scbC
UseSynthetic BiologyTags6xHis and 6xHis-thrombinExpressionBacterialPromoterSynthetic promoter B10, Synthetic promoter B19, a…Available SinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #3
Plasmid#104206PurposeBacterial expression of WW domain #3 from NEDD4LDepositorInsertNEDD4L WW domain #3 (NEDD4L Human)
TagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable SinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
T7-U5 (long)
Plasmid#111337Purposefor in vitro snRNA transcriptionDepositorInsertsnR7-L
ExpressionBacterialAvailable SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
ExpressionBacterialMutationStop codon to glutamine at 23rd amino acidAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pK RagA(Q66L)-RagC
Plasmid#99700PurposeBacterial expression of RagA(Q66L)-RagCDepositorTagsHis tag, Poly Arg, and SUMOExpressionBacterialMutationL91PPromoterT7Available SinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
RNMT
Plasmid#25248PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits