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32,167 results

Showing: 11961 - 12000 of 32167 results

  1. pDOC-K-glmS-mPlum

    Plasmid
    #158062
    Purpose
    Donor plasmid for insertion of mPlum and the constitutive acpP promoter into the S. Typhimurium chromosome. mPlum is in the forward orientation relative to the kanamycin resistance selectable marker
    Depositor
    Mark Webber
    Article
    Holden et al Microbiology (Reading). 2020 Nov 23. doi
    Insert
    monomeric plum fluoresecent protein
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    acpP
    Available Since
    Sept. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    158062-plasmid-map-sequence-id-309442

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  8. pSL0746 (pDonor_R-MmeI)

    Plasmid
    #130646
    Purpose
    Encodes a mini-transposon derived from V. cholerae Tn6677 CAST, with CmR cargo gene. The mutated right end introduces an MmeI recognition site (used for Tn-seq). Total transposon size = 977 bp.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchCAST donor DNA (R*)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Mutation
    TGTTGATA --> TGTTGGAA
    Available Since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    130646-plasmid-map-sequence-id-255642

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  10. TARBP1

    Plasmid
    #25505
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    TARBP1 (TARBP1 Human)
    Tags
    His
    Expression
    Bacterial
    Available Since
    Sept. 10, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. pMT-MCS-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80058
    Purpose
    MCS for cloning promoter to drive HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Tags
    3x HA
    Expression
    Bacterial
    Available Since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80058-plasmid-map-sequence-id-152592

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  20. HSPA5A

    Plasmid
    #42447
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HSPA5A (HSPA5 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  22. pGBW-m4046789

    Plasmid
    #145660
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;StrepII
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available Since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145660-plasmid-map-sequence-id-298333

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  23. DLC1A

    Plasmid
    #25333
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DLC1:S1074-L1283 (DLC1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Contains amino acids S1074-L1283
    Available Since
    Aug. 17, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pET22-6His-SUMO-Nsp10

    Plasmid
    #154960
    Purpose
    Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli
    Depositor
    Justin Kollman
    Insert
    6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
    Tags
    6His-Thrombin-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    154960-plasmid-map-sequence-id-306350

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  26. rrsB-RFP

    Plasmid
    #63838
    Purpose
    This plasmid is designed as a bacterial nutrient sensor
    Depositor
    Howard Salis
    Insert
    rrsB, mRFP1 and GFPmut3b
    Tags
    mRFP1
    Expression
    Bacterial
    Mutation
    Gene is transcriptionally fused to RFP
    Available Since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    63838-plasmid-map-sequence-id-106561

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  28. BAZ2B

    Plasmid
    #39000
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BAZ2B (BAZ2B Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pRS415-KEX2

    Plasmid
    #10996
    Depositor
    Francisco Malagon
    Insert
    KEX2 (KEX2 Budding Yeast)
    Expression
    Bacterial and Yeast
    Mutation
    Wild type
    Available Since
    Dec. 29, 2005
    Availability
    Academic Institutions and Nonprofits only
    10996-plasmid-map-sequence-id-6831

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  33. pCJ206

    Plasmid
    #162679
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating the E226T mutation to the putative lipase box.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), incorporating the E226T mutation to the putative lipase box
    Tags
    His
    Expression
    Bacterial
    Mutation
    E226T; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162679-plasmid-map-sequence-id-321456

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  40. pETM-60-NusA-DmBelle_B

    Plasmid
    #145963
    Purpose
    Bacterial Expression of DmBelle
    Depositor
    Elisa Izaurralde
    Insert
    DmBelle (bel Fly)
    Expression
    Bacterial
    Mutation
    del 155-157, T530A and I685V
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    145963-plasmid-map-sequence-id-333991

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Showing: 11961 - 12000 of 32167 results