30,888 results

1. pBJ3

   Plasmid

   #167132

   Purpose

   A kanamycin resistant plasmid for Tn7-mediated chromosomal integration of Photorhabdus luminescens luxCDABE operon with Pfrr promoter

   Depositor

   Akira Mine

   Article

   Matsumoto et al Plant Commun. 2021 Jul 20;3(1):100227. d

   Insert

   luxCDABE
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   Frr promoter

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU1_000

   Plasmid

   #186773

   Purpose

   * Engineered U1 snRNA plasmid for insertion of guide sequences.

   Depositor

   Eugene Yeo

   Article

   Hatch et al Methods. 2022 Jun 25;205:140-148. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U1 Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFEndoNA

   Plasmid

   #174724

   Purpose

   Expresses GFP-endoNA fusion protein for degradation of polysialic acid. The endoNA is a catalytically active endosialidase from bacteriophage PK1A.

   Depositor

   Jukka Finne

   Article

   Jokilammi et al J Immunol Methods. 2004 Dec;295(1-2):149

   Insert

   GFP-endoNA fusion protein
   

   Tags

   Histag

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLRD01

   Plasmid

   #208257

   Purpose

   Production of terpentetriene in E. coli MEV20

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Inserts

   tHMGR
   cyc1
   cyc2
   

   Expression

   Bacterial

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. hnRNPA2_LC_WT

   Plasmid

   #98657

   Purpose

   Expresses 6xHis-tagged hnRNPA2 AA 190-341

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al Mol Cell. 2018 Jan 17. pii: S1097-2765(1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   Jan. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDV5 containing Sb-convex

   Plasmid

   #132696

   Purpose

   pRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curve

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

   Insert

   Sb_convex including 5' and 3' flanking sequences used for ribosome display
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINVHH_snap_his

   Plasmid

   #210407

   Purpose

   For expression of VHH with C-terminal snap protein and his tag

   Depositor

   Ray Owens

   Article

   Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Promoter

   lac

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCYP01

   Plasmid

   #212834

   Purpose

   Expresses the Rhizobium aethiopicum LRD-modifying cytochrome P450 enzyme

   Depositor

   Christopher Voigt

   Article

   Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

   Insert

   cytochrome P450
   

   Expression

   Bacterial

   Promoter

   PcymRC

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. SUMO_LPETG_MT3

   Plasmid

   #200304

   Purpose

   Bacterial expression plasmids for sortase mediated purification of recombinant MT3. Contains His-tag for purification and SUMO-tag for better solubility and LPETG for sortase mediated cleavage

   Depositor

   Artur Krężel

   Article

   Singh et al Microb Cell Fact. 2023 Jul 11;22(1):125.

   Insert

   metallothionein 3 (MT3 Human)
   

   Tags

   Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRK793

    Plasmid

    #8827

    Depositor

    David Waugh

    Article

    Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

    Insert

    TEV protease, S219V mutant
    

    Tags

    His, MBP (with TEV), and polyarginine

    Expression

    Bacterial

    Mutation

    S219V mutation (improves stability)

    Available Since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. MP6

    Plasmid

    #69669

    Purpose

    Untargetted Bacterial Mutagenesis

    Depositor

    David Liu

    Article

    Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

    Insert

    dnaQ926 dam seqA emrR ugi CDA1
    

    Expression

    Bacterial

    Promoter

    E. coli PBAD

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG/MNase

    Plasmid

    #123461

    Purpose

    Expresses Protein A and Protein G IgG binding domains fused to micrococcal nuclease for purposes of CUT&RUN. Includes (his)6 tag for purification and HA-tag for secondary immunoprecipitation.

    Depositor

    Steven Henikoff

    Article

    Meers et al Elife. 2019 Jun 24;8. pii: 46314. doi: 1

    Insert

    Protein A-Protein G-Micrococcal Nuclease
    

    Tags

    6xHIS and HA

    Expression

    Bacterial

    Promoter

    Lac

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTS117 His14-Avi-45xGS-anti GFP nanobody

    Plasmid

    #199370

    Purpose

    Bacterial expression plasmid for anti-GFP nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    anti-GFP nanobody
    

    Tags

    14xHis-Avi

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T5-LacO

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAV0286

    Plasmid

    #149333

    Purpose

    Bacterial expression of SENP_EuB protease

    Depositor

    Dirk Görlich

    Article

    Vera Rodriguez et al J Cell Biol. 2019 Jun 3;218(6):2006-2020

    Insert

    SENP_EuB protease
    

    Tags

    His14 and Tev protease cleavage site

    Expression

    Bacterial

    Available Since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDisCoTune

    Plasmid

    #173713

    Purpose

    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.

    Depositor

    Morten Norholm

    Article

    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2

    Inserts

    ERV1 (ERV1 Budding Yeast)
    PDI
    

    Expression

    Bacterial

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pACYC-GroEL/ES-TF

    Plasmid

    #83923

    Purpose

    Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

    Depositor

    Karl Griswold

    Article

    Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

    Inserts

    Gro EL/ES
    Trigger Factor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28b-Cas9-His

    Plasmid

    #47327

    Purpose

    For in vitro expression and purification of Cas9 protein

    Depositor

    Alex Schier

    Article

    Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. SpyCatcher003-sfGFP

    Plasmid

    #133449

    Purpose

    Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyCatcher003-sfGFP
    

    Tags

    His6 and TEV site

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBR-Cas9ΔyqhD-HA500

    Plasmid

    #220369

    Purpose

    A single-plasmid-based, easily cured CRISPR/Cas9 system for genome engineering in Pseudomonas putida KT2440

    Depositor

    Yilan Liu

    Article

    Wen et al Microb Cell Fact. 2024 Dec 30;23(1):349.

    Inserts

    SpCas9
    sgRNA for yqhD in P. putida KT2440
    

    Use

    CRISPR; Broad host range

    Expression

    Bacterial

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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