32,417 results
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Plasmid#26624DepositorInsertBirA
TagsHisExpressionBacterialAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET29a-YS14
Plasmid#66890PurposePolycistronic coexpression of Xenopus laevis histones (H2A, H2B, H3 and H4)DepositorInsertsHis6-Thrombin-Xenopus laevis histones H2A
Xenopus laevis histones H2B
Xenopus laevis histones H3
Xenopus laevis histones H4-Thrombin
TagsH2A, H2B, H3, H4, His6 tag, and ThrombinExpressionBacterialPromoterT7Available SinceSept. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-TS2126 RNA Ligase
Plasmid#119941PurposeExpress TS2126 RNA ligase in E. coliDepositorInsertTS2126 RNA ligase
TagsHis6ExpressionBacterialPromoterT7Available SinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEcgRNA
Plasmid#166581PurposeCRISPR-Cas9-assisted genome editing in Escherichia coliDepositorInsertccdB
ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEcCas
Plasmid#73227PurposeConstitutive expression of cas9 and inducible expression of lambda RED and sgRNADepositorInsertsCas9
sgRNA
UseCRISPR; Red recombinaseExpressionBacterialPromoternative promoterAvailable SinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET29b-10xHis-Super TEV
Plasmid#193833PurposeExpresses high-efficiency TEV protease in bacterial cellsInsertTEV protease
Tags6xHisExpressionBacterialMutationT17S, L56V, N68D, I77V, S135G, I138T, S153N, T180…Available SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSANG10-3F-BG4
Plasmid#55756PurposeExpresses BG4 scFv anti-G-quadruplex antibody in bacteriaDepositorInsertBG4
Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialPromoterT7Available SinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET29-bdSUMO-Anti-ALFA nanobody
Plasmid#212311PurposeExpression of anti-ALFA nanobodyDepositorInsertanti-ALFA nanobody
TagsbdSUMOExpressionBacterialPromoterT7Available SinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-Tn5
Plasmid#60240PurposeIPTG-inducible expression of Transposon Tn5 fused to Mxe Intein and Chitin-binding domain.DepositorInserttnpA from pBam1
TagsMxe intein - Chitin-binding domainExpressionBacterialMutationE54K, L372PPromoterT7 promoterAvailable SinceNov. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTS118 His14-Avi-28xGS-anti ALFA tag nanobody
Plasmid#199371PurposeBacterial expression plasmid for anti-ALFA nanobody with an N-terminal His-tag and biotin acceptor peptide (Avi)DepositorInsertanti-ALFA nanobody
Tags14xHis-AviExpressionBacterialMutationWTPromoterT5-LacOAvailable SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
Raf-1 GST RBD 1-149
Plasmid#13338DepositorAvailable SinceOct. 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-pAG-Hia5
Plasmid#174373PurposePlasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein AG - hia5
Tags6xHis and Protein A/GExpressionBacterialAvailable SinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB/mStayGold
Plasmid#212017PurposemStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.DepositorInsertmStayGold
ExpressionBacterialPromoterT7Available SinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pA_higher-Opto_N449S
Plasmid#253258PurposeOpto-CheY N449S expressed using the proA promoter and wild type T7 RBSDepositorInsertOpto-CheY with N449S mutation
Tagswild type T7 RBSExpressionBacterialAvailable SinceMarch 19, 2026AvailabilityAcademic Institutions and Nonprofits only -
pJR98
Plasmid#187239PurposeCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sitesDepositorInsertCR3 constant region - hU6 sgRNA promoter flanked by BsmBI sites
ExpressionBacterialAvailable SinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0286
Plasmid#149333PurposeBacterial expression of SENP_EuB proteaseDepositorInsertSENP_EuB protease
TagsHis14 and Tev protease cleavage siteExpressionBacterialAvailable SinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAR17_StrepI_hFASh_H8_pET22b
Plasmid#122846PurposeExpresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.DepositorInserthuman FASN/ human fatty acid synthase (FASN Human)
TagsHis8 tag and Twin-Strep tagExpressionBacterialMutationwildtypePromoterT7 promoterAvailable SinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHO4d-Cas9
Plasmid#67881PurposeCas9nlsHis6 Bacterial Expression constructDepositorInsertCas9
UseCRISPRTagsHis6 and T7 epitopeExpressionBacterialPromoterT7Available SinceSept. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHT-ads
Plasmid#47382PurposeExpresses ads under Pgrac promoter (inducible by IPTG) in bacteriaDepositorInsertads
Tags6xHisExpressionBacterialPromoterPgracAvailable SinceOct. 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
HLTV-hTRIM21
Plasmid#104973PurposePurification of His-Lipoyl-hTRIM21 protein from bacteriaDepositorInsertTripartite Motif containing 21 (TRIM21 Human)
TagsHis-LipoylExpressionBacterialPromoterT7Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only