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Showing: 1 - 20 of 29429 results

  4. sgRNA_expression_vector

    Plasmid
    #210212
    Purpose
    An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.
    Depositor
    Albert Jeltsch
    Article
    Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    210212-plasmid-map-sequence-id-416400

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  6. esmGFP-C10-pVTCB052

    Plasmid
    #231303
    Purpose
    Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli
    Depositor
    Alexander Rives
    Insert
    esmGFP-C10
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231303-plasmid-map-sequence-id-463619

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  13. pDisCoTune

    Plasmid
    #173713
    Purpose
    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
    Depositor
    Morten Norholm
    Article
    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2
    Inserts
    ERV1 (ERV1 Budding Yeast)
    PDI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    173713-plasmid-map-sequence-id-342928

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  14. pCas

    Plasmid
    #62225
    Purpose
    Constitutive expression of cas9 and inducible expression of lambda RED and sgR
    Depositor
    Sheng Yang
    Article
    Jiang et al Appl Environ Microbiol. 2015 Jan 30. pii
    Has Service
    Cloning Grade DNA
    Insert
    cas9
    Use
    CRISPR; Lambda red recombinase
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    native cas9 promoter
    Available since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    62225-plasmid-map-sequence-id-275267

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  18. pET-pA-Hia5

    Plasmid
    #174372
    Purpose
    SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Article
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein A - hia5
    Use
    Tags
    6xHis and Protein A
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174372-plasmid-map-sequence-id-341860

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Showing: 1 - 20 of 29429 results