4,932 results

1. ninaE-norpA

   Plasmid

   #26854

   Depositor

   Craig Montell

   Article

   Wang et al Neuron. 2008 Jan 10. 57(1):56-68.

   Insert

   no receptor potential A (norpA Fly)
   

   Expression

   Insect

   Mutation

   V963A

   Available Since

   April 5, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pCFD2-dU6:2gRNA

   Plasmid

   #49409

   Purpose

   expression of gRNA under control of the Drosophila U6:2 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-2:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-2

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pUAS-syb:spGFP1–10

   Plasmid

   #73698

   Purpose

   Neuronal-synaptobrevin (syb):spGFP1–10 chimera for synaptic GRASP

   Depositor

   Marco Gallio

   Article

   Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

   Insert

   Neuronal-synaptobrevin:spGFP1–10 chimera
   

   Use

   Drosophila

   Expression

   Insect

   Available Since

   March 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1 INI1

   Plasmid

   #1953

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   INI1 (SMARCB1 Human)
   

   Tags

   flag

   Expression

   Insect

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

5. pUb FLAG-Human IntS6

   Plasmid

   #198408

   Purpose

   Expresses FLAG-tagged human IntS6

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   DDX26 (INTS6 Human)
   

   Tags

   3x FLAG

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAcGP67A-TfR

   Plasmid

   #12130

   Depositor

   Pamela Bjorkman

   Article

   Lebron et al Cell. 1998 Apr 3. 93(1):111-23.

   Insert

   transferrin receptor (TFRC Human)
   

   Tags

   6X-His tag, Factor Xa cleavage site, and leader p…

   Expression

   Insect

   Mutation

   The portion of the human TfR gene encoding residu…

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pFl Strep-sumo-TEV-hAgo2

   Plasmid

   #136235

   Purpose

   Expresses human Argonaute-2 in insect cells

   Depositor

   Leemor Joshua-Tor

   Article

   Elkayam et al Cell. 2012 Jul 6;150(1):100-10. doi: 10.

   Insert

   human Argonaute-2 (AGO2 Human)
   

   Tags

   Strep Sumo

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUASTattB-QS

   Plasmid

   #24366

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QS
   

   Expression

   Insect

   Available Since

   May 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mVenus-PA-Rac1

   Plasmid

   #22007

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Tags

   6X His and mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

   Promoter

   CMV, p10

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pKM3

    Plasmid

    #154168

    Purpose

    Empty vector with FLAG tag expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFl Strep-Sumo-TEV human OAS1

    Plasmid

    #130895

    Purpose

    Human oligoadenylate synthase 1 (OAS1) is used to label terminal ADP-riobos

    Depositor

    Leemor Joshua-Tor

    Article

    Ando et al Mol Cell. 2019 Feb 21;73(4):845-856.e5.

    Insert

    oligoadenylate synthase 1 (OAS1 Human)
    

    Tags

    Strep_Sumo

    Expression

    Insect

    Promoter

    ph

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pOPINS3C

    Plasmid

    #41115

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSR651

    Plasmid

    #65213

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-1 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPiggyBacATCC LacZ GCTT loxAttP

    Plasmid

    #173496

    Purpose

    piggyBac insect transgenesis destination vector for Golden Gate Cloning assemblies

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDyn1

    Plasmid

    #64067

    Purpose

    Human cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).

    Depositor

    Andrew Carter

    Article

    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:

    Insert

    DYNC1H1 (DYNC1H1 Human)
    

    Use

    Cre/Lox

    Tags

    His-ZZ-TCS-SNAPf

    Expression

    Insect

    Mutation

    Codon optimized for Sf9

    Available Since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAST_mLExA-VP16-NFATc1 (C-del)

    Plasmid

    #35023

    Depositor

    Jing-W Wang

    Insert

    NFATc1 (NFATC1 Human)
    

    Tags

    VP16 and mLexA

    Expression

    Insect

    Mutation

    deletion of amino acids 590-716

    Promoter

    UAS

    Available Since

    March 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTriEx-RhoA FLARE.sc Biosensor Q63L

    Plasmid

    #12151

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor Q63L (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA Q63L (dominant-positive mutation)

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGP-JFRC59-13XLexAop2-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162383

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TH1204-pOCC120-FUS_opt(NheI)

    Plasmid

    #221892

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Tags

    MBP-PreScission and mGFP-PreScission-6xHis

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available Since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pOmnibac_ZZ_linker_K963_GFP_SNAP

    Plasmid

    #159727

    Purpose

    Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
    

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Available Since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. SGyA

    Plasmid

    #160292

    Purpose

    Drives expression of Cas9 and a TdTomato fluorescent marker

    Depositor

    Omar Akbari

    Article

    Gamez et al Nat Commun. 2021 Dec 10;12(1):7202. doi:

    Inserts

    Vas promoter
    hSpCas9
    tdTomato
    CTCF insulator
    Gypsy insulator
    Left homology arm Y chromosome
    Right homology arm Y chromosome
    

    Tags

    T2A GFP

    Expression

    Insect

    Available Since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBPGAL4.1Uw

    Plasmid

    #26226

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4::p65 (RELA Human, Budding Yeast)
    

    Expression

    Insect

    Available Since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTriEx-mCherry::LANS4

    Plasmid

    #60785

    Purpose

    Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation

    Depositor

    Brian Kuhlman

    Article

    Yumerefendi et al PLoS One. 2015 Jun 17;10(6):e0128443. do

    Insert

    LANS4
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAc5.1-V5-HisB-ObKHC-wt-HaloTag

    Plasmid

    #201551

    Purpose

    Expresses wild-type Octopus kinesin-1 motor with HaloTag in Drosophila S2 cells

    Depositor

    Joshua Rosenthal

    Article

    Birk et al Cell. 2023 Jun 8;186(12):2544-2555.e13.

    Insert

    Kinesin-1 (partial)
    

    Tags

    HaloTag

    Expression

    Insect

    Mutation

    Codon-optimized for Drosophila expression

    Promoter

    Ac5

    Available Since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 57F03_LexA-GAD

    Plasmid

    #83256

    Purpose

    Expresses LexA-GAD under the control of 57F03 enhancer

    Depositor

    Leslie Vosshall

    Article

    Yapici et al Cell. 2016 Apr 21;165(3):715-29. doi: 10

    Insert

    57F03 enhancer
    

    Expression

    Insect

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pQF2wWB

    Plasmid

    #61313

    Purpose

    Enhancer trap version of QF2w, analogous to pGAWB

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF2w
    

    Expression

    Insect

    Available Since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. UAS-Syt::Halo7

    Plasmid

    #87648

    Purpose

    For creating drosophila transgenics expressing UAS-Syt::Halo7

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    Syt::Halo7
    

    Expression

    Insect

    Mutation

    S265G in Syt (please see depositor's commen…

    Available Since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAV5B+longBRD4

    Plasmid

    #157792

    Purpose

    Expression of long isoform of BRD4

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    long BRD4 (BRD4 Human)
    

    Tags

    His6

    Expression

    Insect

    Promoter

    polyhedron

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCFD1-dU6:1gRNA

    Plasmid

    #49408

    Purpose

    expression of gRNA under control of the Drosophila U6:1 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-1:gRNA
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    dU6-1

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Promoter

    GAL4-responsive UAS hsp70

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTriEx-RhoA FLARE.sc Biosensor T19N

    Plasmid

    #12152

    Depositor

    Klaus Hahn

    Article

    Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

    Insert

    RhoA Biosensor T19N (RHOA Human)
    

    Tags

    6xHis

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    RhoA T19N (dominant-negative mutation)*

    Available Since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pOPING

    Plasmid

    #26046

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    SS peptide-LacZ-KHHHHHH
    

    Tags

    KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    May 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGP-20XUAS-IVS-Syn21-jGCaMP8m-p10

    Plasmid

    #162387

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8m
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOPINE-3C-eGFP

    Plasmid

    #41125

    Depositor

    Ray Owens

    Insert

    GFP
    

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCRISPaint-3xP3-RFP

    Plasmid

    #130276

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes 3xP3-RFP visible eye marker and multiple cloning site.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    3xP3-RFP
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pOmniBac zz TEV YBBR TRF1

    Plasmid

    #185445

    Purpose

    Expresses human TRF1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site in insect cells

    Depositor

    Ahmet Yildiz

    Article

    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

    Insert

    TRF1 (TERF1 Human)
    

    Tags

    YBBR and ZZ

    Expression

    Insect

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pF3BGX-T2A-p65-AD

    Plasmid

    #138395

    Purpose

    RMCE vector for generating C-terminal T2A-p65-AD fusion (split GAL4)

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-p65-AD
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAC-U63-QtgRNA2.1-BR

    Plasmid

    #170513

    Purpose

    gRNA cloning vector containing a BFP Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

    Depositor

    Chun Han

    Article

    Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6:3

    Available Since

    June 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge