4,932 results
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Plasmid#118412PurposeExpresses firefly luciferase under the control of a strong ubiquitous promoter EF1a along with a WPRE cassette. Backbone is compatible with both baculoviral and human production methods of AAV.DepositorInsertFirefly luciferase
UseAAV, Luciferase, and Synthetic BiologyExpressionInsect and MammalianPromoterEF1aAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q62C-A140C
Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 …PromoterpolyhedringAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCaryP
Plasmid#18945DepositorInsertphiC31 attP
ExpressionInsectAvailable SinceOct. 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII
Plasmid#217897PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tagDepositorTags2xSTIIExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7
Plasmid#217898PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7)DepositorAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHD-3xP3-dsRed-DattP
Plasmid#133559PurposeBackbone vector for CRISPR-HDR insertion of SPARC-Variant constructsDepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL_CORO1C:1-474
Plasmid#210920PurposeInsect expression of full-length human CORO1C. N-terminal 6X His tag with TEV cleavage.DepositorInsertCORO1C:M1-A474 (CORO1C Human)
TagsMHHHHHHSSGRENLYFQGExpressionInsectMutationwild typePromoterPolyhedrinAvailable SinceMarch 20, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmG3BP_G
Plasmid#146349PurposeInsect Expression of DmG3BPDepositorInsertDmG3BP (rin Fly)
ExpressionInsectMutationV632G compared to AF231031.1, exact match to NP_5…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-let-7 4x
Plasmid#20930DepositorInsert4x let-7 target sites
UseLuciferaseExpressionInsect and MammalianAvailable SinceJune 19, 2009AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-mtdT-3XHA
Plasmid#24354DepositorInsertmtDT-3XHA
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDmAlfaTub84b
Plasmid#165781PurposeDrosophila Alpha Tubulin promoterDepositorInsertAlfaTub84b
ExpressionInsectAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427V
Plasmid#81044Purposemammalian expression of LOV2 I427VDepositorInsertLOV I427V
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427VAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-MZT1
Plasmid#178075PurposeInsect cell expression of MZT1DepositorInsertMZT1 (MZT1 Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pActPL-VP16AD
Plasmid#15305DepositorInsertVP16AD-ZIP+
ExpressionInsectAvailable SinceJuly 6, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHRW-CRY2olig-VHH(GFP)
Plasmid#126023Purposeexpress RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogeneticsDepositorInsertCRY2 PHRolig-VHH(GFP)
TagsRFPExpressionInsectMutationCRY2 E490GPromoterHsp70Available SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
UAST-LbCas12a
Plasmid#140621PurposeGal4 dependent expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRExpressionInsectPromoterUASTAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorAvailable SinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-FLPo
Plasmid#24357DepositorInsertFLPo
ExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9L
Plasmid#166788PurposeInsect cell expression vector for DPP9L (protein purification)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
ExpressionInsectAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto Grx1-roGFP2
Plasmid#64994Purposefor expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophilaDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K560_GFP_SNAP
Plasmid#159720PurposeExpresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-560), K560 (KIF5B Human)
TagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids 1-560Available SinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST tdTomato Profilin WT
Plasmid#53509DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAttB-Tub
Plasmid#203966PurposeFly transformation vector with Tubulin promoter and SV40 PA site and a UTR for ubiquitous expressionDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFL His-mEos3.2-I3
Plasmid#113516PurposeExpression of His-Eos-I3 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
56 pLIB_CBP
Plasmid#160305PurposeN-term 3C cleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneTagsCBPExpressionInsectPromoterpolyhydrinAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_H11
Plasmid#184277PurposeExpresses nanobody H11 in E. coliDepositorInsertNanobody H11
TagsHis6ExpressionBacterial, Insect, and Mamm…PromoterT7Available SinceFeb. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9S
Plasmid#166787PurposeInsect cell expression vector for DPP9S (protein purification)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
His-ZZ-TEV-SNAPf-BICD2N(1-400)
Plasmid#111857PurposeN-terminal SNAPf tag on BICD2N (1-400) for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.DepositorInsertBICD2N(1-400)
TagsHis-tag, SNAPf-tag, TEV site, and ZZExpressionInsectMutationOptimised for Sf9 expressionAvailable SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBac-GH146-QF
Plasmid#24353DepositorInsertQF
TagsdsRedExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA-GFP_rescue_construct
Plasmid#190641PurposePuromycin-selectable expression of GFP in Drosophila S2 cellsDepositorInsertEGFP
Tags3xHAExpressionInsectPromoterActin-5cAvailable SinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RABIF-FLAG
Plasmid#135558PurposeExpress human RABIF in mammalian cells, FLAG at N terminalDepositorAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-SBP_J
Plasmid#146621PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-V5-SBP_J
Plasmid#146633PurposeInsect Expression vectorDepositorTypeEmpty backboneExpressionInsectAvailable SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
mTubulin NLS-mCherry-LEXY
Plasmid#182597PurposeDrosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertmCherry
TagsLEXY and NLSExpressionInsectAvailable SinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
OA-1067A1
Plasmid#164847PurposeExpresses four gRNA targeting BTub gene with 3xp3-eGFP markerDepositorInsert4 gRNAs and 3xP3-eGFP
ExpressionInsectAvailable SinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only