5,038 results
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Plasmid#111157PurposeBaculovirus rescue vector, VSVGED pseudotype, hShhDepositorInserthBmp4 (BMP4 Human)
UseBaculoviral rescue shuttle vectorExpressionInsect and MammalianMutationwtPromoterCMVAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
HS-H4-GFP
Plasmid#8699DepositorAvailable SinceJune 30, 2005AvailabilityAcademic Institutions and Nonprofits only -
EKAR2G_design2_mTFP_105_Venus_195
Plasmid#40002DepositorInsertErk activity reporter
TagsHis-MycExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-GAL4DBD
Plasmid#26264DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pOCC94
Plasmid#118912Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6DepositorInsertNcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and eGFP-HIS6ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfCsf1a
Plasmid#168103PurposeExpress soluble His-tagged zebrafish Csf1a in insect cellsDepositorAvailable SinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRIV-Gal4DBD:2xnMagHigh1
Plasmid#182031PurposeDonor plasmid for conversion of KO attP Drosophila lines into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1-p103'UTR
UseDonor plasmidExpressionInsectAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF2
Plasmid#162524PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
ExpressionInsectAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Jag1ICD-HA pBacPAK8-3
Plasmid#17331DepositorAvailable SinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
TagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-NbVHH05-hIgG
Plasmid#171565PurposeExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
TagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-16
Plasmid#104966PurposePlasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAsDepositorInsertRibozyme-gRNA-ribozyme
UseCre/LoxExpressionInsectAvailable SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
TagsTurboIDExpressionInsectPromoterUAS-Gal4Available SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 WWE-PARP
Plasmid#192550PurposeE. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
TagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…PromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKM499
Plasmid#134216PurposeBaculovirus expression of CENP-U CENP-Q CENP-OPDepositorInserthis-CENPU + CENPQ+ CENPOP
ExpressionInsectAvailable SinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X79-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) M188L
Plasmid#159692PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) M188LDepositorInsertHis6-MBP-tev-G-Hs.KRAS4b(1-188) M188L (KRAS Human)
TagsHis6-MBP-tevExpressionInsectMutationM188LAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVMG0146_Cq-U6-1_2xBbsI-gRNA
Plasmid#169238PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertCq-U6-1_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039653Available SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVMG0149_Cq-U6-6_2xBbsI-gRNA
Plasmid#169323PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0149_Cq-U6-6_2xBbsI-gRNA
UseCRISPRExpressionInsectPromoterCPIJ039596Available SinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACSV-Ptc
Plasmid#37675DepositorAvailable SinceNov. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
BacPAK Flag-NFRKB
Plasmid#19791DepositorAvailable SinceDec. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorAvailable SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p13xLexAOP2-IVS-Syn21-Voltron-p10
Plasmid#119042PurposeLexAop2-driven expression of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSK24
Plasmid#72852Purposetemplate for C-terminal Twin-Strep tag, Puromycin-resistanceDepositorInserttwin Strep tag
ExpressionInsectAvailable SinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC
Plasmid#71812PurposeGenerating transgenic flies for FLP-inducible DamIDDepositorInsertmin.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
ExpressionInsectAvailable SinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
CG4845/pMT/V5-HisB
Plasmid#17589DepositorAvailable SinceApril 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
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ULK3 (ULK3A-c002)
Plasmid#110272PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
Moe-pDONR221
Plasmid#124239PurposeGateway entry clone of Drosophila Moesin cDNA encoding the D isoform.DepositorInsertDrosophila Moesin (Moe Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXN-FBLWLF-QF
Plasmid#26267DepositorAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-CeALG1_O
Plasmid#147147PurposeInsect Expression of CeALG1DepositorInsertCeALG1
ExpressionInsectAvailable SinceMarch 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pfast NPC1(NTD)
Plasmid#25773DepositorAvailable SinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
Hook3(1-522)-SNAPf-Psc-StrepII
Plasmid#111860PurposeC-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.DepositorInsertHook3(1-552)-SNAPf (HOOK3 Human)
TagsPsc, SNAPf, and Strep-IIExpressionInsectMutationOptimised for expression in Sf9 cellsAvailable SinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-mos::NG
Plasmid#138958PurposeUsed to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4DepositorArticleInsertkkv (NEWENTRY Fly)
UseUas transgene vectorTagsNeon Green fused to C terminus of KkvExpressionInsectMutation8 bp that are in the region that encodes the cata…PromoterUASAvailable SinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-dsRed::FRT-RC,attB}
Plasmid#182177Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-eGFP::FRT-RC,attB}
Plasmid#182175Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT-RC,attB}
Plasmid#182181Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct:MS2-p65-HSF
Plasmid#78902PurposeExpresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cellsDepositorInsertMS2-p65-HSF
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab4 WT
Plasmid#37690DepositorAvailable SinceJune 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-6His-Nter-GWs-Lox (VE5587)
Plasmid#163768PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 6 His tag under the pH promoter.DepositorInsertN-terminal 6His tag
Tags6 His TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-10His-Cter (VE5631)
Plasmid#161802PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the p10 promoter.DepositorInsertC-terminal 10 His tag
Tags10 HisExpressionInsectPromoterp10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-10His-Cter (VE5742)
Plasmid#139776PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter10His tag under the pH promoter.DepositorInsertC-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-6His-Cter (VE5630)
Plasmid#139777PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.DepositorInsertC-terminal 6His tag
Tags6 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-c-Myc-Cter (VE5632)
Plasmid#139778PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.DepositorInsertC-terminal C-Myc tag
Tagsc-MycExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-Flag-Cter(VE5738)
Plasmid#161798PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the p10 promoter.DepositorInsertC-terminal Flag tag
TagsFlagExpressionInsectPromoterp10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-pH-Flag-Cter (VE5739)
Plasmid#161799PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.DepositorInsertC-terminal Flag tag
TagsFlagExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)
Plasmid#161800PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.DepositorInsertN-terminal HA tag
TagsHAExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-10His-Nter-GWs-Lox (VE5588)
Plasmid#161806PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter 10 His tag under the pH promoter.DepositorInsertN-terminal 10His tag
Tags10 HisExpressionInsectPromoterPHAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_MF-p10-mCh PH-mCh (VE5625)
Plasmid#139769PurposeTransfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an mCherry cDNA under each promoter.DepositorInsertmCherry fluorescent protein
ExpressionInsectPromoterPH or p10Available SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
Or30a-Gal4
Plasmid#63027PurposeTo express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or30a, using the promoter sequences of the gene cloned 5' of Gal4DepositorInsertDrosophila melanogaster Or30a promoter
TagsDrosophila ADH (aa's 1-18) fused to Yeast Ga…ExpressionInsectAvailable SinceApril 22, 2015AvailabilityAcademic Institutions and Nonprofits only -