4,932 results

1. pFB1_hMSH2_G674A

   Plasmid

   #134874

   Purpose

   Expression human MSH2 G674 mutant protein using baculovirus

   Depositor

   Peggy Hsieh

   Article

   Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

   Insert

   human MSH2_G674A (MSH2 Human)
   

   Expression

   Insect

   Mutation

   Changed Glycine to Alanine at aa#674

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pACSV-Ptc

   Plasmid

   #37675

   Depositor

   Philip Beachy

   Article

   Yao et al Cell. 2006 Apr 21;125(2):343-57.

   Insert

   ptc (ptc Fly)
   

   Expression

   Insect

   Promoter

   Actin 5C

   Available Since

   Nov. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. HS-H4-GFP

   Plasmid

   #8699

   Depositor

   Kami Ahmad

   Article

   Ahmad et al Mol Cell 2002 Jun;9(6):1191-200.

   Insert

   H4 (His4:CG31611 Fly)
   

   Tags

   GFP

   Expression

   Insect

   Available Since

   June 30, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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4. bTub-GFP.766A

   Plasmid

   #112695

   Purpose

   expressed GFP in testes, under bTub promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   bTub-GFP-SV40
   

   Expression

   Insect

   Promoter

   bTub

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-CmTMC1truncated

   Plasmid

   #154908

   Purpose

   For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-CmTMC1truncated
   

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-243 and 843-969

   Available Since

   Sept. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-MuTMC2truncated

   Plasmid

   #154909

   Purpose

   For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.

   Depositor

   Motoyuki Hattori

   Article

   Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d

   Insert

   GFP-MuTMC2truncated
   

   Tags

   8histidine-EGFP

   Expression

   Insect

   Mutation

   Truncations of the residues 1-179 and 691-716

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pfast NPC1(NTD)

   Plasmid

   #25773

   Depositor

   Johann Deisenhofer

   Article

   Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

   Insert

   npc1 (NPC1 Human)
   

   Tags

   His

   Expression

   Insect

   Mutation

   aa 23-252 only

   Available Since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pOCC254

   Plasmid

   #118889

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEV

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
   

   Tags

   HIS6-MBP, cleavable with 3C protease; mCherry, cl…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Jag1ICD-HA pBacPAK8-3

   Plasmid

   #17331

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

   Insert

   Jagged1 (Jag1 Rat)
   

   Tags

   HA

   Expression

   Insect

   Available Since

   Feb. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

10. RIPK2 (RIPK2A-c036)

    Plasmid

    #110268

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RIPK2 (RIPK2 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSK24

    Plasmid

    #72852

    Purpose

    template for C-terminal Twin-Strep tag, Puromycin-resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    twin Strep tag
    

    Expression

    Insect

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycPC

    Plasmid

    #71812

    Purpose

    Generating transgenic flies for FLP-inducible DamID

    Depositor

    Bas van Steensel

    Article

    Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

    Insert

    min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-PC
    

    Expression

    Insect

    Available Since

    March 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. Hook3(1-522)-SNAPf-Psc-StrepII

    Plasmid

    #111860

    Purpose

    C-terminal SNAPf tag on Hook3 (amino acids 1-522) for expression in Sf9 cells. C-terminal SNAPf-Psc-StrepII tag.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    Hook3(1-552)-SNAPf (HOOK3 Human)
    

    Tags

    Psc, SNAPf, and Strep-II

    Expression

    Insect

    Mutation

    Optimised for expression in Sf9 cells

    Available Since

    July 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. AT5G48740 O3_pECIA2

    Plasmid

    #114963

    Purpose

    Bait vector AT5G48740 O3_pECIA2 should be used with prey vector AT5G48740 O3_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G48740 (AT5G48740 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AT5G59650 O4_pECIA2

    Plasmid

    #114964

    Purpose

    Bait vector AT5G59650 O4_pECIA2 should be used with prey vector AT5G59650 O4_pECIA14.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT5G59650 (AT5G59650 Mustard Weed)
    

    Tags

    Fc, V5 and hexahistidine tags

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFS337-USP44-FLAG

    Plasmid

    #43905

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

    Insert

    USP44 (USP44 Human)
    

    Use

    Baculoviral expression

    Tags

    FLAG

    Expression

    Insect

    Available Since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pLib6.4B-Aaeg_774

    Plasmid

    #176673

    Purpose

    Expression of sgRNA under Ae. Aegypti U6 (AAEL017774) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLib6.4B-Cquin_596

    Plasmid

    #176669

    Purpose

    Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Expression

    Insect

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBSR

    Plasmid

    #165838

    Purpose

    blasticidin S resistance marker

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    bsr
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. 1xTRE DREaMR 3p3GFP

    Plasmid

    #182203

    Purpose

    1xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.

    Depositor

    Craig Montell

    Article

    Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

    Insert

    rTetR-3XP3-GFP
    

    Expression

    Insect

    Available Since

    April 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pXN-FBLWLF-QF

    Plasmid

    #26267

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    QF (qf Neurospora crassa)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCoofy59

    Plasmid

    #122011

    Purpose

    Baculovirus vector for parallel SLIC cloning containing N-terminal TrxA-His6 with PreScission Cleavage site

    Depositor

    Peggy Stolt-Bergner, Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    TrxA-His6

    Expression

    Insect

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAct:MS2-p65-HSF

    Plasmid

    #78902

    Purpose

    Expresses MS2-p65-HSF under actin promoter for SAM CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    MS2-p65-HSF
    

    Use

    CRISPR

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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24. CG4845/pMT/V5-HisB

    Plasmid

    #17589

    Depositor

    Denise Montell

    Insert

    CG4845 (psidin Fly)
    

    Tags

    6x His and V5

    Expression

    Insect

    Available Since

    April 3, 2008

    Availability

    Academic Institutions and Nonprofits only

25. pUASP YFP Rab4 WT

    Plasmid

    #37690

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab4 (Rab4 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    June 10, 2013

    Availability

    Academic Institutions and Nonprofits only

26. pIE2-AD

    Plasmid

    #21603

    Depositor

    Takahiro Kusakabe

    Article

    Mon et al Anal Biochem. 2009 Sep 15;392(2):180-2.

    Insert

    NF-kappa-B p65 subunit (Rela Mouse)
    

    Use

    Gateway destination vector

    Tags

    Flag, Gateway destination cassette, and NLS

    Expression

    Insect

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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27. pOPINTolB

    Plasmid

    #41138

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFastBac1-OSF-TRIM5alpha(human)(R332P)

    Plasmid

    #79037

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(human R332P) (TRIM5 Human)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Changed Arg 332 to Pro

    Promoter

    polyhedrin

    Available Since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. HBM-mVEGF-C-HisTag-pENTR

    Plasmid

    #64066

    Purpose

    Plasmid encodes mature form of murine VEGF-C preceded by honey bee melittin secretion signal; entry vector to Gateway cloning system.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

    Insert

    vascular endothelial growth factor C (Vegfc Mouse)
    

    Tags

    His-tag

    Expression

    Insect

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUASP dsRed Rab5 DN

    Plasmid

    #44823

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab5 (Rab5 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    S43N (dominant negative)

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

31. pAW-EGFP-4xlet-7 (pAW-AS27)

    Plasmid

    #40834

    Purpose

    miRNA sensor used in Drosophila cell lines

    Depositor

    Phillip Zamore

    Article

    Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

    Insert

    Four target sites perfectly complementary to let-7
    

    Use

    Mirna reporter

    Tags

    EGFP

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

32. pQUAST-p65AD::CaM

    Plasmid

    #64715

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    p65AD::CaM
    

    Expression

    Insect

    Available Since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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33. RP78 pUAST Orco mRNA

    Plasmid

    #53431

    Purpose

    Expresses the full-length Drosophila melanogaster Orco cDNA under Gal4/UAS control

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    Orco (Orco )
    

    Expression

    Insect

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pBGRY

    Plasmid

    #61599

    Purpose

    Transformation vector pBGRY

    Depositor

    John Carlson

    Article

    Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

    Type

    Empty backbone

    Use

    Drosophila transformation vector

    Expression

    Insect

    Available Since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSLfa[Hsp_p-GAL4Delta-SV40;attp]fa

    Plasmid

    #86451

    Purpose

    Gal4 driver construct for misexpression in Tribolium with Tribolium basal promoter; has been used for enhancer trapping; (Shuttle plasmid)

    Depositor

    Gregor Bucher

    Insert

    Hsp_p-GAL4Delta-SV40;attp
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCFD3-MS2tail-SAM

    Plasmid

    #78905

    Purpose

    Vector for expressing a single sgRNA with SAM MS2 tail, under U6:3 promoter

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Aug. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pUASp YFP Rab4 CA

    Plasmid

    #53466

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab04
    

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q67L

    Promoter

    GAL4

    Available Since

    June 4, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pUAST-HA, Pbl-A

    Plasmid

    #69792

    Purpose

    Pebble isoform A in P element-based pUAST vector for Gal4-regulated expression in Drosophila.

    Depositor

    Arno Muller

    Article

    van Impel et al Development. 2009 Mar;136(5):813-22. doi

    Insert

    Pebble - Drosophila guanine nucleotide exchange factor
    

    Use

    P element-based puast vector for gal4-regulated e…

    Tags

    HA tag

    Expression

    Insect

    Mutation

    Encodes Pbl (NP_729306.1) lacking last 9 amino ac…

    Promoter

    hsp70 promoter

    Available Since

    Nov. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

39. pMT-GFP-Sas4

    Plasmid

    #31756

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Sas4 (Sas-4 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Mutation

    N70T

    Promoter

    Metallothionein

    Available Since

    Jan. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

40. DYNLL1

    Plasmid

    #132530

    Purpose

    Human LC8-1, codon optimised for Sf9 expression.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Insert

    DYNLL1 (DYNLL1 Human)
    

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Available Since

    Jan. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge