4,932 results

1. Hy_pUbi-p63e mCherry SV40

   Plasmid

   #176300

   Purpose

   Expresses mCherry mRNA from the Drosophila Ubi-p63e promoter

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   mCherry
   

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

   Plasmid

   #71805

   Purpose

   Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]
   

   Expression

   Insect

   Available Since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDR121

   Plasmid

   #37148

   Depositor

   Dale Ramsden

   Insert

   XRCC6 (XRCC6 Human)
   

   Tags

   H6

   Expression

   Insect

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

   Plasmid

   #214137

   Purpose

   Insect cell expression of the human 20SCP proteasome assembly chaperones

   Depositor

   Brenda Schulman

   Article

   Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

   Inserts

   POMP (POMP Human)
   PSMG1 (PSMG1 Human)
   PSMG2 (PSMG2 Human)
   PSMG3 (PSMG3 Human)
   PSMG4 (PSMG4 Human)
   

   Expression

   Insect

   Promoter

   polyhedrin

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDR123

   Plasmid

   #37149

   Depositor

   Dale Ramsden

   Insert

   XRCC5 (XRCC5 Human)
   

   Expression

   Insect

   Available Since

   July 18, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pAC-Luc

   Plasmid

   #33154

   Depositor

   Michael Rosbash

   Article

   Kadener et al Genes Dev. 2007 Jul 1;21(13):1675-86. Ep

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Insect

   Promoter

   Actin 5C

   Available Since

   Dec. 8, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pHsp83-NLS-HA-2xMCP-2xTagRFP-T

   Plasmid

   #71242

   Purpose

   Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

   Depositor

   Anne Ephrussi

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   NLS-2xMCP-2xTagRFP-T
   

   Use

   P element-mediated germline transformation

   Tags

   TagRFP-T

   Expression

   Insect

   Promoter

   Hsp83

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQF2WB

   Plasmid

   #61312

   Purpose

   Enhancer trap version of QF2, analogous to pGAWB

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF2
   

   Expression

   Insect

   Available Since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. PRE-Hsp70BbCas9_1.3

   Plasmid

   #190797

   Purpose

   aka PRE-Hsp70BbCas9 or 2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with two gypsy insulator elements. Final product

   Depositor

   Omar Akbari

   Article

   Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

   Insert

   2gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
   

   Expression

   Insect

   Available Since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFastBac Dual His6 MBP N10 TEV LIC cloning vector (5C)

    Plasmid

    #30123

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-MBP-N10-TEV

    Expression

    Insect

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac1-melHis10TEV

    Plasmid

    #159428

    Purpose

    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites

    Depositor

    Debra Hansen

    Type

    Empty backbone

    Tags

    melittin signal sequence (removed in vivo by sign…

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUCDMp10

    Plasmid

    #170461

    Purpose

    EarlyBac insect cell expression system

    Depositor

    Hiro Furukawa

    Article

    Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. do

    Insert

    New vector
    

    Expression

    Insect

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid

    #22028

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-ADARcd-E488Q-V5

    Plasmid

    #139686

    Purpose

    Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

    Depositor

    Michael Rosbash

    Article

    Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

    Insert

    Adenosine deaminase acting on RNA (Adar Fly)
    

    Tags

    V5

    Expression

    Bacterial and Insect

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTP2620

    Plasmid

    #113311

    Purpose

    human Rad50 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Lee et al J Biol Chem. 2013 May 3;288(18):12840-51

    Insert

    Rad50 (RAD50 Human)
    

    Tags

    HA, 6xhis

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. R702-X80-635: His6-tev-Hs.NF1opt (1-2818) isoform 2

    Plasmid

    #159575

    Purpose

    Baculovirus protein expression of His6-tev-Hs.NF1opt (1-2818) isoform 2

    Depositor

    Dominic Esposito

    Article

    Sherekar et al J Biol Chem. 2020 Jan 24;295(4):1105-111

    Insert

    His6-tev-Hs.NF1opt (1-2818) isoform 2 (NF1 Human)
    

    Tags

    His6-tev

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTP813

    Plasmid

    #113308

    Purpose

    human Mre11 in baculovirus transfer vector

    Depositor

    Tanya Paull

    Article

    Bhaskara et al Mol Cell. 2007 Mar 9;25(5):647-61. doi:

    Insert

    Mre11 (MRE11 Human)
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBac1-Nucleocapsid

    Plasmid

    #179696

    Purpose

    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells

    Depositor

    Mark Arbing

    Insert

    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    His6-TEV protease site

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCiB-yin

    Plasmid

    #13845

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    yellow (y Fly)
    

    Expression

    Insect

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLAG-XBP1u-FLuc

    Plasmid

    #31239

    Depositor

    Jimtong Horng

    Article

    Jheng et al Cell Microbiol. 2010 Jun . 12(6):796-813

    Insert

    FLAG-XBP1u-FLuc (XBP1 Human)
    

    Tags

    Flag and Fluc

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

21. PRExpress

    Plasmid

    #122486

    Purpose

    A non-leaky heat-inducible gene expression system for Drosophila. (GenBank: MF058598.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Inserts

    LacZ
    poly-PRE
    

    Expression

    Insect

    Available Since

    May 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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22. 15x-QUAS-dTomato-T2A-GCaMP6s

    Plasmid

    #130666

    Purpose

    QUAS reporter line that expresses cytosolic dTomato and the GCaMP6s calcium indicator, separated by T2A

    Depositor

    Leslie Vosshall

    Article

    Matthews et al eLife. 2019 May 21;8. pii: 43963. doi: 1

    Insert

    15xQUAS-dTomato-T2A-GCaMP6s
    

    Use

    Ae. aegypti expression

    Expression

    Insect

    Promoter

    15xQUAS-TATA

    Available Since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. pJFRC12-10XUAS-IVS-myr::GFP

    Plasmid

    #26222

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    myr::GFP
    

    Expression

    Insect

    Available Since

    Oct. 19, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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24. pACUH

    Plasmid

    #58374

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Sapar et al Cell Rep. 2018 Aug 28;24(9):2273-2286. d

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pKRG3-mU6-PUb-hSpCas9-pAc

    Plasmid

    #162163

    Purpose

    Expresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectable

    Depositor

    Charles Rice

    Article

    Rozen-Gagnon et al Sci Rep. 2021 Jan 12;11(1):736. doi: 10.

    Inserts

    hSpCas9
    sgRNA
    

    Use

    CRISPR

    Tags

    T2A-pAc

    Expression

    Insect

    Promoter

    Ae. aegypti PUb and Ae. aegypti U6

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGP-20XUAS-IVS-Syn21-jGCaMP8s-p10

    Plasmid

    #162386

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Insert

    jGCaMP8s
    

    Tags

    6xHis

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available Since

    May 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFlpStop-attB-UAS-2.1-tdTom

    Plasmid

    #88910

    Purpose

    attB FlpStop plasmid, for insertion into MiMIC site

    Depositor

    Tom Clandinin

    Article

    Fisher et al Elife. 2017 Feb 17;6. pii: e22279. doi:

    Insert

    FlpStop
    

    Expression

    Insect

    Promoter

    5XUAS

    Available Since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pOCC102

    Plasmid

    #118883

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C protease

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-MBP-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6-MBP, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pOCC151

    Plasmid

    #118899

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal GST, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-GST-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    GST tag, cleavable with 3C protease

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAC-hRluc

    Plasmid

    #24348

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    hRluc
    

    Expression

    Insect

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUA

    Plasmid

    #58372

    Purpose

    UAS vector to express gene of interest in Drosophila

    Depositor

    Chun Han, Yuh-Nung Jan

    Article

    Han et al Neuron. 2014 Feb 5;81(3):544-60. doi: 10

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTriEx-PA-Rac1

    Plasmid

    #22024

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Tags

    6X His

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

33. nSYB-GAL80-DD

    Plasmid

    #124778

    Purpose

    Expresses GAL80 fused with destabilizing domain (DD) downstream of a n-Synaptobrevin promoter

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized GAL80 (GAL80-DD)
    

    Tags

    DD

    Expression

    Insect

    Promoter

    nSynaptobrevin

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTF023_His_hsDDA1

    Plasmid

    #174480

    Purpose

    Expresses DDA1 in insect cells

    Depositor

    Eric Fischer

    Article

    Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

    Insert

    DDA1 (DDA1 Human)
    

    Tags

    His-TEV

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFastBac_His-ZZ-TEV-Lis1-SNAPf

    Plasmid

    #133073

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

    Depositor

    Simon Bullock, Andrew Carter

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Tags

    8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pUASTP2.1

    Plasmid

    #13842

    Depositor

    Ting Wu

    Article

    Bateman et al Genetics. 2006 Jun . 173(2):769-77.

    Insert

    mini-white (w Fly)
    

    Expression

    Insect

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PRExpress-Bxb1-hsp70pA

    Plasmid

    #158391

    Purpose

    heat shock inducible Bxb1 integrase for drosophila melanogaster

    Depositor

    Michael Elowitz

    Article

    Chow et al Science. 2021 Apr 9;372(6538). pii: 372/

    Insert

    Bxb1
    

    Expression

    Insect

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. IR25a-T2A-QF-AD

    Plasmid

    #162526

    Purpose

    Plasmid for CRISPR-mediated knock-in line that expresses the activation domain of QF by knocking-in a T2A-QF-AD fragment replacing the stop codon of the endogenous Aedes aegypti Ir25a gene

    Depositor

    Leslie Vosshall

    Article

    Herre et al Cell. 2022 Aug 18;185(17):3104-3123.e28.

    Insert

    Ir25a-left-HDR-arm; T2A-QF2AD-Zip+-SV40; 3xP3-EYFP-SV40; Ir25a-right-HDR-arm
    

    Expression

    Insect

    Available Since

    Aug. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pSR646

    Plasmid

    #65215

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-6 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-6 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pACgp67B-Her2

    Plasmid

    #10794

    Depositor

    Judy Lieberman

    Article

    Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

    Insert

    ErbB2 ScFv (ERBB2 Human)
    

    Tags

    6His

    Expression

    Insect

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge