4,958 results

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pLIB-HIS-TEV-CKS1

Plasmid

#177013

Purpose

Generate baculovirus for insect cell expression of CKS1 with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

CKS1 (CKS1B Human, Synthetic)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL4 (GAL4 Budding Yeast)

Expression

Insect

Available Since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pVL1393-6xMyc

Plasmid

#128035

Purpose

Baculovirus expression vector with C-terminal 6xMyc tag

Depositor

Oskar Laur

Type

Empty backbone

Tags

6xMyc tag

Expression

Insect

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pLIB-Cyclin K

Plasmid

#231718

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

CCNK (CCNK Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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Orco-T2A-QF2-9xQUAS-GCaMP6f-3XP3-dsRed

Plasmid

#157974

Purpose

Template plasmid for inserting GCaMP reporter into the Orco gene of Aedes aegypti with CRISPR/Cas9

Depositor

Carolyn McBride

Article

Zhao et al Nature. 2022 May;605(7911):706-712. doi:

Insert

Orco

Expression

Insect

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGRACE

Plasmid

#230910

Purpose

Tracing of CRISPR activity in whole animals.

Depositor

Michael Boutros

Article

Port et al bioRxiv 2024.11.26.625385

Insert

GFP

Use

CRISPR

Expression

Insect

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_CDC20:161-477

Plasmid

#210943

Purpose

Insect expression of human CDC20 fragment S161 to P477. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC20:S161-P477 (CDC20 Human)

Tags

MHHHHHHSSGRENLYFQG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pFBD-BirA_CDC20:161-P477

Plasmid

#210888

Purpose

Insect expression of human CDC20 fragment S161 to P477. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

CDC20:S161-P477 (CDC20 Human)

Tags

GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

Expression

Insect

Mutation

wild type

Promoter

Polyhedrin

Available Since

March 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLIB-FLAG(N)-RPB1

Plasmid

#231722

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

POLR2A (POLR2A Human)

Tags

Flag-3C

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:D127-E814 (EIF3B Human)

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRDB_053

Plasmid

#216083

Purpose

for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31

Use

Destination

Expression

Insect

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-UAP56 WT

Plasmid

#110125

Purpose

Expresses Flag-tagged human UAP56 (DDX39B)

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

UAP56 (DDX39B Human)

Tags

Flag

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac-ATG101-ATG13 HORMA

Plasmid

#99332

Purpose

Mammalian expression of one subcomplex from the Human ULK1 complex

Depositor

James Hurley

Article

Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

Inserts

ATG101 (ATG101 Human)
ATG13 (ATG13 Human)

Tags

GST

Expression

Insect

Promoter

p10 and pH

Available Since

Aug. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pLIB-WDR61

Plasmid

#231729

Purpose

Protein expression in insect cells

Depositor

Jesper Svejstrup

Article

Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

Insert

WDR61 (SKIC8 Human)

Expression

Insect

Available Since

May 2, 2025

Availability

Academic Institutions and Nonprofits only

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pACUH-GFP11x7-mCherry-α-tubulin

Plasmid

#70218

Purpose

Expresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11x7-mCherry-α-tubulin

Tags

GFP11x7 and mCherry

Expression

Insect

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pOPINJ

Plasmid

#26045

Depositor

Ray Owens

Article

Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

Insert

HisGST3C-lacZ

Tags

MAHHHHHH_GST_LEVLFQGT

Expression

Bacterial, Insect, and Mamm…

Available Since

Oct. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Vector 1174D

Plasmid

#200012

Purpose

Fluorescent based sex separator in Aedes aegypti (SEPARATOR)

Depositor

Omar Akbari

Article

Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.

Insert

Engineered dobulesex splicing module

Use

Synthetic Biology

Expression

Insect

Mutation

Engineered dobulesex splicing module

Promoter

Hr5IE1

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 8x

Plasmid

#20931

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

8x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available Since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFastbac1-Flag-FANCD2

Plasmid

#134904

Purpose

codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

FANCD2 (FANCD2 Human)

Tags

Flag

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

polyhedron

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pGE-attBTT-loxP-DsRed (pTL780)

Plasmid

#115160

Purpose

Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

attB-TT loxP 3xP3-DsRed

Use

Cre/Lox

Expression

Insect

Available Since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-yki:V5-S111A, S168A, S250A

Plasmid

#85620

Purpose

Expresses V5 tagged triple mutant yki in Drosophila.

Depositor

Kenneth Irvine

Article

Oh et al Oncogene. 2009 Apr 30;28(17):1916-27. do

Insert

yki-V5-S111A,S168,S250A

Tags

V5

Expression

Insect

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pBac{3XP3::EYFP,attP}

Plasmid

#86860

Purpose

Expresses EYFP in eyes and contains an attP landing site for site-specific attB integration

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Insert

attP

Expression

Insect

Available Since

June 9, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDmAct5C::Cas9-2A-Neo

Plasmid

#176679

Purpose

Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

D. melanogaster Act5C

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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