5,046 results
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Plasmid#46164PurposeExpression of murine CD8mCherry in insectsDepositorInsertmCD8mCherry
ExpressionInsectAvailable SinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJFRC151-3XUAS-IVS-Flp2::PEST
Plasmid#32133DepositorInsertFlippase2::PEST
TagsPESTExpressionInsectMutationGlycine at Amino Acid 5Available SinceSept. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
piB-GFP
Plasmid#13844DepositorInsertTATA-GFP-SV40
ExpressionInsectAvailable SinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKM7
Plasmid#154164PurposemCherry expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.DepositorInsertmCherry
ExpressionInsectPromoterIE1 promoter and hr5 enhancer from Autographa cal…Available SinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDC-UAS-nBitbow1.0(miniW)
Plasmid#138329PurposeDrosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.DepositorInsertsmAmetrine
tdKatushka2
mNeonGreen
mTFP1
mKusabira-Orange2
Tagshuman H2BExpressionInsectPromoter10xUASAvailable SinceMarch 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSimpleII-U6-sgRNA-BsmBI-NLS-NmCas9-HA-NLS
Plasmid#115694PurposeAll-in-one plasmid. Contains expression cassette for NmeCas9 with N and C NLS and HA tag at the C, plus cassette (for cloning of spacer in BsmBI) for expressing sgRNA under the control of U6 promoter.DepositorInsertsNmeCas9
Nme-sgRNA
UseCRISPRTagsNLS and NLS, HAExpressionInsect and MammalianMutationNone and fusion of repeat/tracrRNA to make a sgRNAPromoterEF-1 alpha and U6Available SinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUCDM
Plasmid#110742PurposePlasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP siteDepositorTypeEmpty backboneExpressionInsectPromoterPolyhedrin and p10 promotersAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmec-4::Chrimson::SL2::mCherry::unc-54
Plasmid#107745PurposeExpress Chrimson and mCherry in mechanosensory neurons.DepositorInsertsChrimson
mCherry
ExpressionInsect and WormPromotermec-4Available SinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFlpStop-HDR-UAS-2.1-tdTom
Plasmid#89148PurposeFlpStop plasmid for CRISPR/HDR. Has MCS for homology armsDepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoter5XUAS, 3XP3-dsRedAvailable SinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDyn1
Plasmid#64067PurposeHuman cytoplasmic dynein heavy chain (DYNC1H1) codon optimized for Sf9 expression with a SNAPf tag for fluorescent labeling and a TEV-cleavable His-ZZ tag for purification (His-ZZ-TCS-SNAPf-DYNC1H1).DepositorInsertDYNC1H1 (DYNC1H1 Human)
UseCre/LoxTagsHis-ZZ-TCS-SNAPfExpressionInsectMutationCodon optimized for Sf9Available SinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5
Plasmid#63168PurposeGAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporterDepositorInsert"spaghetti monster GFP-V5"
TagsN-myristoylationExpressionInsectPromoterGAL4-responsive UAS hsp70Available SinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pActP-GCaMP6m
Plasmid#64714Purposevisualizing calcium in cultured Drosophila S2 cellsDepositorInsertGCaMP6m
ExpressionInsectAvailable SinceMay 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnos-Fok1:dCas9-nos
Plasmid#62210PurposeExpresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.DepositorInsertFok1-dCas9
ExpressionInsectPromoternanosAvailable SinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJFRC4-3XUAS-IVS-mCD8::GFP
Plasmid#26217DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
pQUAST>stop>mCD8-GFP
Plasmid#24361DepositorInsertmCD8-GFP
ExpressionInsectAvailable SinceApril 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUAS-Luc
Plasmid#21604DepositorInsertUAS-hsp70 luciferase
ExpressionInsectAvailable SinceSept. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1 Flag Baf170
Plasmid#1955DepositorAvailable SinceJuly 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDW464
Plasmid#8845DepositorTypeEmpty backboneTagsbiotin acceptor peptideExpressionInsectAvailable SinceNov. 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9-pAc
Plasmid#162163PurposeExpresses hSpCas9-T2A-pAc (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter); puromycin selectableDepositorInsertshSpCas9
sgRNA
UseCRISPRTagsT2A-pAcExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIDS-LSD1fl
Plasmid#109157PurposeEncodes human full-length LSD1 to be expressed in a baculovirus/insect cell expression systemDepositorInserthuman full-length LSD1, sequence-optimized for insect cells (KDM1A Human)
ExpressionInsectPromoterp10Available SinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-hDicer
Plasmid#89144PurposeExpression of hDicer in Sf9 CellsDepositorAvailable SinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
TagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
tubP-FRT>GAL80-6-FRT>
Plasmid#63173Purposeubiquitous expression of a "flp-Out" Drosophila codon-optimized GAL80 gene cassetteDepositorInsert"Flp-Out" GAL80
ExpressionInsectAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMH5
Plasmid#52531Purposeexpresses FLP recombinase in DrosophilaDepositorAvailable SinceApril 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pE4n
Plasmid#17458Purposemodified pENTR vector for N-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGST and Thrombin siteExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A-TfR
Plasmid#12130DepositorInserttransferrin receptor (TFRC Human)
Tags6X-His tag, Factor Xa cleavage site, and leader p…ExpressionInsectMutationThe portion of the human TfR gene encoding residu…Available SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDW465
Plasmid#8846DepositorTypeEmpty backboneTagsbiotin acceptor peptideExpressionInsectAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
20XUAS IVS CsChrimson tdtomato_tr
Plasmid#111544PurposeOptogenetics in Drosophila: UAS-induced expression of Chrimson tdTomatoDepositorInsertChrimson_tdtomato_trafficked
ExpressionInsectMutationwtPromoterhsp70Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC-20X-UAS-ADARcd
Plasmid#81173Purposeidentification of cell-specific targets of RNA binding proteinsDepositorInsertUAS-ADARcd
ExpressionInsectPromoterUASAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only