81,094 results
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pLifeAct-mScarlet3_N1
Plasmid#189767PurposeConstruct for labelling actin filaments in mammalian cells with lifeact-mScarlet3DepositorInsertLifeAct-mScarlet3
ExpressionMammalianPromoterCMVAvailable SinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-GfaABC1D-Cre-4x6T
Plasmid#196410PurposeAstrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassetteDepositorHas ServiceAAV5InsertCre
UseAAV; Astrocyte-selectiveExpressionMammalianPromoterCAG and GfaABC1DAvailable SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-FIRE-pHLy
Plasmid#170774PurposeRatiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cellsDepositorInsertLAMP1 (LAMP1 Human)
UseLentiviralTagsmCherry and mTFP1ExpressionMammalianMutation84 nucleotide Signal Sequence switched to the beg…PromoterhUbCAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorAvailable SinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMOS023: Peredox NADH/NAD+ sensor (cytosolic)
Plasmid#163060PurposePeredox NADH/NAD+ sensor (cytosolic)DepositorInsertPeredox
UseLentiviralTagsmCherryExpressionMammalianPromoterCMVAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-FLEX-jGCaMP8f-WPRE
Plasmid#162379PurposeAAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expressionDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertjGCaMP8f
UseAAV and Cre/LoxTags6xHisExpressionMammalianMutationQ315LPromoterSynapsinAvailable SinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
NES-EGFP-cPHx3
Plasmid#116855PurposePI(3,4)P2 biosensorDepositorInsertPLEKHA1 (PLEKHA1 Human)
TagsEGFP and X.leavis map2k1.L(32-44)ExpressionMammalianMutationamino acids 169-329 (tandem trimer)Available SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-ASC1
Plasmid#75134PurposeExpresses N-terminal Flag-tagged ASC1 in mammalian cellsDepositorInsertPYD and CARD domain containing (Pycard Mouse)
TagsFLAGExpressionMammalianMutationF23S (please see depositor comments below)PromoterCMVAvailable SinceAug. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-wtYAP
Plasmid#84009Purposeexpresses wtYAP in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianPromoterTRE-CMVAvailable SinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+ hnRNPK myc HIS
Plasmid#71662PurposeMammalian expression of hnRNPKDepositorAvailable SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_iSH2-pMag(3x)-iRFP
Plasmid#67304PurposeBlue light dependent photoswitching systemDepositorAvailable SinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
5'UTR CGG 99x FMR1-EGFP
Plasmid#63091PurposeContains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.DepositorInsertexpanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
PX855
Plasmid#62887PurposeN-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)DepositorInsertSpCas9 (aa 2-535)
UseCRISPRTagsFRB and NES PTK2ExpressionMammalianMutationAspartic acide 10 to Alanine (D10A)PromoterCBhAvailable SinceMarch 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-puro U6 sgRNA BfuAI large stuffer
Plasmid#52628Purposelentiviral U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites, improved cassette cloning efficiencyDepositorInsertKanamycin cassette
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV6 BDNF
Plasmid#39857DepositorAvailable SinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
iRFP-P4M-SidM
Plasmid#51470PurposeBiosensor for PtdIns4PDepositorInsertP4M-SidM(546-647)
TagsiRFPExpressionMammalianPromoterCMVAvailable SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-parkin C431S
Plasmid#45877DepositorInsertParkin (PRKN Human)
TagsEGFPExpressionMammalianMutationC431S mutation (ubiquitin acceptor site)PromoterCMVAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP(N3)-PP1beta
Plasmid#44223DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
EGFP-hCaM
Plasmid#47602DepositorAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-FLAG-XRCC4
Plasmid#46959PurposePlasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 (N-terminal tag). Confers resistance to G418.DepositorAvailable SinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
p75-RFP
Plasmid#24092DepositorAvailable SinceJan. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKAR2-CR
Plasmid#40255DepositorInsertAKAR2
TagsHis6 and XpressExpressionMammalianPromoterCMV IEAvailable SinceOct. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
PJ-DEAD
Plasmid#38002DepositorTagsmRFPExpressionMammalianMutationAsp 1263 mutated to Ala and Cys 779 mutated to SerAvailable SinceAug. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3
Plasmid#32739DepositorInsertTCF4E (Tcf7l2 Human)
TagsMycExpressionMammalianMutationThe N-terminal deletion form (delta N) lacks amin…Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTRE-Slow-FT
Plasmid#31915DepositorInsertSlow-FT
ExpressionMammalianMutationM18V/E30V/K69R/L84W/A179V compared to mCherry (bu…PromoterPTight Tet-responsive PminCMVdAvailable SinceDec. 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSADdeltaG-F3
Plasmid#32634DepositorInsertB19N, B19P, B19M and B19L
ExpressionMammalianMutationIn frame insertion of Ser after aa10 in M (matrix…PromoterCMVAvailable SinceOct. 26, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS3 (CK2alpha, CK2beta)
Plasmid#27092DepositorUseTetracycline-regulated expressionTagsHA and MycExpressionMammalianPromoterbidirectional tet-responsive promoterAvailable SinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
NGFR
Plasmid#27489DepositorTypeEmpty backboneUseRetroviralTagsECMV, IRES, and NGFRExpressionMammalianAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLenti CMVtight eGFP Puro (w771-1)
Plasmid#26431Purpose2nd generation lentiviral vectorDepositorInsertEnhanced Green Fluorescent Protein
UseLentiviral; Tet-on advanced vectorExpressionMammalianAvailable SinceJan. 6, 2011AvailabilityAcademic Institutions and Nonprofits only
