81,094 results
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TGFB1_pLX307
Plasmid#98377PurposeLentiviral expression of TGFB1DepositorAvailable SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TLCV2-RB1
Plasmid#87836PurposeLentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.DepositorInsertsgRB1 (RB1 Human)
UseCRISPR and Lentiviral; Doxycycline inducible; egf…ExpressionMammalianPromoterTight TRE promoter and U6Available SinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBK-YEE-BE3
Plasmid#85177PurposeExpresses YEE-BE3 in mammalian cellsDepositorInsertYEE-BE3
ExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP
Plasmid#86457PurposeMouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sitesDepositorInsertBFP
UseCRISPRExpressionMammalianPromoterPgkAvailable SinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
Tags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…PromoterCMVAvailable SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBMN(CMV-copGFP-Puro-SMAR)
Plasmid#80391Purposeexpression copGFP and Puromycine, with an S/MAR element for episomal maintainceDepositorInsertcopGFP-T2A-Puromycine
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19
Plasmid#78597PurposeExpress flag-USP19 in mammalian cellsDepositorAvailable SinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-STAT3-Y705F
Plasmid#74434PurposeExpression of STAT3-Y705FDepositorInsertSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
TagsFlagExpressionMammalianMutationMutations E16K, Y705FPromoterCMVAvailable SinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRubiG-T2A-Cas9
Plasmid#75348PurposeUbiquitin promoter expresses GFP and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsGFP (via t2a)ExpressionMammalianPromoterUbiquitinAvailable SinceMay 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS Exon Vector
Plasmid#69893PurposeExpression plasmid for expressing circular RNAs of a desired sequence in mammalian cellsDepositorAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR (ANV)
Plasmid#71684PurposeExpression of mutant human codon-optimized dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the inactivated (E756A) catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Human, Synthetic)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterCBhAvailable SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-CFP-VGPDFGR-YFP
Plasmid#59587PurposeEncodes a granzyme B FRET reporterDepositorInsertECFP-VGPDFGR-Venus
UseRetroviralTagsECFP and VenusExpressionMammalianPromoterCMVAvailable SinceNov. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
MOS
Plasmid#64120PurposeA modified episomal (EBNA1/oriP) vector expressing human OCT4 and SOX2 genesDepositorAvailable SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Ii-Str_ssSBP-EGFP
Plasmid#65277Purposeto synchronize transport of secretory soluble GFP from the ER (RUSH system)DepositorInsertEGFP
TagsIL-2 signal sequence and Streptavidin Binding Pr…ExpressionMammalianPromoterCMVAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNeuroD-ires-GFP
Plasmid#61403PurposeMammalian expression vector under the neuron-specific promoter NeuroD. Co-expression of GFP with IRES sequenceDepositorTypeEmpty backboneExpressionMammalianPromoterNeuroDAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
AAV:ITR-U6-sgRNA(LacZ)-pCBh-Cre-WPRE-hGHpA-ITR
Plasmid#60228PurposeExpresses Cre recombinase from the Cbh promoter and one U6-driven sgRNA control targeting LacZ. AAV backbone.DepositorInsertssgRNA
Cre recombinase
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HAExpressionMammalianPromoterCBh and U6Available SinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-Golgi-7
Plasmid#54108PurposeLocalization: Golgi Complex, Excitation: 487, Emission: 509DepositorInsertGolgi (B4GALT1 Human)
TagsmEmeraldExpressionMammalianMutationaa 1-82 of NM_001497.3PromoterCMVAvailable SinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mEos2-N1
Plasmid#54662PurposeLocalization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneTagsmEos2ExpressionMammalianAvailable SinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCS-memb-mCherry
Plasmid#53750PurposeContains mCherry fused to a 2x membrane localization sequence in the pCS2+ vector.DepositorInsert2x membrane linker
TagsmCherryExpressionMammalianPromotersimian CMV IE94Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Abl-His6-FLAG
Plasmid#52684Purposeexpression of Abl in mammalian cellsDepositorAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
TagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable SinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hCas9
Plasmid#51142PurposeExpresses hCas9 under the CAG promoter for CRISPRDepositorInserthCas9
UseCRISPRTagsSV40 NLSExpressionMammalianPromoterCAGAvailable SinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
FLAG-VAMP8
Plasmid#45912DepositorInsertvesicle-associated membrane protein 8 (VAMP8 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable SinceDec. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-mC/EBPb
Plasmid#49198Purposeexpression of mouse C/EBPbeta in mammalian cellsDepositorAvailable SinceNov. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Kl-EGFP
Plasmid#45532DepositorAvailable SinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS2+8CmCherry
Plasmid#34935DepositorTypeEmpty backboneUseSea urchin, zebrafish, xenopusTagsmCherryExpressionMammalianPromoterSP6Available SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
m168
Plasmid#34886DepositorInsertSindbis virus envelope protein
ExpressionMammalianPromoterCMVAvailable SinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPSmOrange2-N1
Plasmid#34958DepositorInsertPSmOrange2
ExpressionMammalianMutationR17H, R36H, F64I, Q194L, A224S, G226A (mutations …Available SinceAug. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-N LMP2A
Plasmid#37963DepositorInsertLMP2A (LMP-2A H. Herpesvirus 4 (EBV))
UseRetroviralTagsFlag and HAExpressionMammalianPromoterPKGAvailable SinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only
