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Mammalian Expression

76,413 results

Showing: 9701 - 9750 of 76413 results

  1. C1-MPAct-mCitrine

    Plasmid
    #155220
    Purpose
    Monitor changes in the density of membrane-proximal F-actin (MPA)
    Depositor
    Tobias Meyer
    Article
    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210
    Insert
    F-tractin (aa 9-52 of rat ITPKA) fused to mCitrine C-term tagged with the CaaX sequence derived from Kras4b
    Use
    Tags
    mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155220-plasmid-map-sequence-id-310146

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  2. pKT2-ACVR1-G328V-IRES-Katushka

    Plasmid
    #135002
    Purpose
    Expresses ACVR1 with a G328V mutation and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Article
    Mendez et al Clin Cancer Res. 2020 Aug 1;26(15):4080-
    Insert
    Activin A receptor, type I (ACVR1) G328V (ACVR1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c.983G>T, p.G328V
    Promoter
    Available since
    July 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    135002-plasmid-map-sequence-id-271117

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  3. pCAGGS-EGFP-V5

    Plasmid
    #200936
    Purpose
    expresses EGFP-V5 in mammalian cells
    Depositor
    Hung-Chun Chang
    Insert
    EGFP
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    April 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    200936-plasmid-map-sequence-id-409428

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  5. pHAGE pGK MitoFlux (pSu9-HaloTag7-mGFP-STOP)

    Plasmid
    #215011
    Purpose
    Express a Mitophagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    pSu9 (Presequence of Fo-ATPase subunit 9)
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215011-plasmid-map-sequence-id-429274

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  7. pHAGE pGK ERFlux (Bip-HaloTag7-mGFP-KDEL-STOP)

    Plasmid
    #215013
    Purpose
    Express a ERphagy reporter in mammalian cells (untested)
    Depositor
    Wade Harper
    Insert
    KDEL
    Use
    Lentiviral
    Tags
    HaloTag(7)-mGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    215013-plasmid-map-sequence-id-429268

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  8. EFS-FLAG-SpCas9-6xNLS(c-Myc)-P2A-Puro (pRG214)

    Plasmid
    #221385
    Purpose
    Lentiviral expression of SpCas9-6xNLS(c-Myc) with puromycin resistance gene
    Depositor
    Junwei Shi
    Insert
    SpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    221385-plasmid-map-sequence-id-435944

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  9. pT2-3X-FLAG-H3.1-K27M-BFP

    Plasmid
    #209439
    Purpose
    SB-transposable plasmid expressing a FLAGGED mutant histone H3.1-K27M with BFP
    Depositor
    Maria Castro
    Insert
    H3.1-K27M
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K27M
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209439-plasmid-map-sequence-id-424832

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  10. pT2-H3.1-WT-BFP

    Plasmid
    #209437
    Purpose
    SB-transposable plasmid expressing FLAGGED human histone mutant H3.1-Wt with BFP
    Depositor
    Maria Castro
    Insert
    H3.1-WT
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 8, 2024
    Availability
    Academic Institutions and Nonprofits only
    209437-plasmid-map-sequence-id-424810

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  12. TFORF0172

    Plasmid
    #142551
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ONECUT2 (ONECUT2 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    June 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    142551-plasmid-map-sequence-id-397438

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  13. pLHCX-ATF4 mScarlet NLS

    Plasmid
    #115970
    Purpose
    ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)
    Depositor
    David Andrews
    Article
    Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:
    Insert
    activating transcription factor 4 (ATF4 Human, Synthetic)
    Use
    Retroviral
    Tags
    HA tag, c-myc NLS, and mScarlet-I
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    115970-plasmid-map-sequence-id-222082

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  21. pJZC78

    Plasmid
    #62339
    Purpose
    sgRNA + 1x COM with COM-KRAB effector for mammalian cells
    Depositor
    Stanley Qi, Wendell Lim
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…
    Promoter
    CMV and U6
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62339-plasmid-map-sequence-id-102262

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  24. Lenti-LucS

    Plasmid
    #22778
    Depositor
    Tyler Jacks
    Insert
    SIY
    Use
    Cre/Lox and Lentiviral
    Tags
    luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 14, 2009
    Availability
    Academic Institutions and Nonprofits only
    22778-plasmid-map-sequence-id-10426

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  25. PB CAG-sfGFP

    Plasmid
    #133568
    Purpose
    CAG sfGFP piggyBac transposon
    Depositor
    Mario Capecchi
    Insert
    sfGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    133568-plasmid-map-sequence-id-274027

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  30. UBC-4xmNG-dMCP

    Plasmid
    #231093
    Purpose
    Encodes a 4xmNeonGreen-dMCP under control of a weak promoter (UBC).
    Depositor
    John Ngo
    Insert
    4xmNG-dMCP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    231093-plasmid-map-sequence-id-458495

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  36. pN7CAST

    Plasmid
    #190661
    Purpose
    Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    190661-plasmid-map-sequence-id-385667

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  38. pDG461

    Plasmid
    #100902
    Purpose
    SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9 Nickase
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A mutant converts to Nickase
    Promoter
    CBh and U6
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100902-plasmid-map-sequence-id-195323

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  49. pcDNA3 DSred express2

    Plasmid
    #128040
    Purpose
    Mammalian expression vector with N terminal DsRed express2
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Tags
    DsRed Express2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    128040-plasmid-map-sequence-id-254863

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Showing: 9701 - 9750 of 76413 results