76,357 results

1. 8xNFAT-ZsG-mCD8

   Plasmid

   #162747

   Purpose

   NFAT-driven ZsGreen-1 reporter gene with mouse CD8 gene

   Depositor

   Maki Nakayama

   Article

   Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

   Insert

   8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP- SEC61B

   Plasmid

   #121159

   Purpose

   Mammalian expression of GFP tagged SEC61B, used as a general ER marker

   Depositor

   Christine Mayr

   Article

   Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

   Insert

   SEC61B (SEC61B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. lenti-SpCas9 blast

   Plasmid

   #104997

   Purpose

   This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

   Depositor

   Brett Stringer

   Article

   Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

   Insert

   KpnI-XhoI-BsrGI-NheI
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

   Plasmid

   #223712

   Purpose

   Expresses autophagy flux LC3B fluorescence reporter

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   LC3B (MAP1LC3B Synthetic, Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry::EGFP::LC3B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-hROSA26-1-1kbdonor

   Plasmid

   #234736

   Purpose

   PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   hROSA26-1 crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX_307

   Plasmid

   #41392

   Purpose

   Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

   Depositor

   David Root

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-miniTurbo-V5-RNASEL_IDG-K

   Plasmid

   #170604

   Purpose

   Gateway destination clone of RNASEL (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-RNASEL (RNASEL Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   June 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-UbC-VP64-dCas9-VP64

   Plasmid

   #232179

   Purpose

   Lentiviral cassette that expresses 2xVP64-dCas9 fusion in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi

   Insert

   VP64-dCas9-VP64
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   hUbC

   Available since

   March 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCherry-SH3(5R)

   Plasmid

   #112154

   Purpose

   Mammalian expression plasmid for SH3-5R in a vector expressing mCherry.

   Depositor

   Michael Rosen

   Article

   Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

   Insert

   SH3-5R (NCK1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   only the second SH3 domain is expressed in this c…

   Promoter

   CMV

   Available since

   Feb. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV/D377Y-mPCSK9

    Plasmid

    #58376

    Purpose

    Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

    Depositor

    Jacob Bentzon

    Article

    Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

    Has Service

    AAV8

    Insert

    proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    D377Y

    Promoter

    HCRApoE/hAAT

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLVpuro-CMV-N-mCherry

    Plasmid

    #123221

    Purpose

    N-terminal mCherry tag. Gateway destination vector for lentivirus production and stable transduction in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. Flag-SENP1

    Plasmid

    #17357

    Depositor

    Edward Yeh

    Article

    Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

    Insert

    SENP1 (SENP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCXWB-EBNA1

    Plasmid

    #37624

    Purpose

    Non-replicating episomal expression of EBNA1, which enhances transfection efficiency and expression from episomal plasmids

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    EBNA-1 (EBNA-1 human herpesvirus 4)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA Raptor

    Plasmid

    #8513

    Depositor

    David Sabatini

    Article

    Kim et al Cell 2002 Jul 26;110(2):163-75.

    Insert

    Raptor (RPTOR Human)
    

    Use

    Tags

    ha

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. mEGFP

    Plasmid

    #18696

    Depositor

    Karel Svoboda

    Article

    Harvey et al Science. 2008 Jul 4. 321(5885):136-40.

    Insert

    monomeric EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    A206K mutation

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV_CKAR3

    Plasmid

    #238413

    Purpose

    AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

    Depositor

    Haining Zhong

    Article

    Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

    Insert

    CKAR3 (PKC sensor)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. TK4-EGFP

    Plasmid

    #239047

    Purpose

    PiggyBac plasmid for stable transgene expression during human pluripotent stem cell differentiation

    Depositor

    Marius Wernig

    Inserts

    EGFP
    puroR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and EF1a

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pXPR_120

    Plasmid

    #96917

    Purpose

    for CRISPRa, lentiviral expression of dCas9-VPR 2A BlastR

    Depositor

    John Doench, David Root

    Article

    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    VPR

    Expression

    Mammalian

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-ExRai-AktAR2

    Plasmid

    #184047

    Purpose

    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    ExRai-AktAR2
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. EGFP-Vimentin-7

    Plasmid

    #56439

    Purpose

    Localization: Intermediate Filaments, Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    Vimentin (VIM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. JNK-KTR-mStrawberry reporter

    Plasmid

    #158689

    Purpose

    This plasmid is a JNK pathway reporter. It has CMV promoter driving the expression of JNK-KTR fused to mStrawberry-pGK-BSD

    Depositor

    Ravid Straussman

    Article

    Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

    Insert

    mStrawberry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCRIS-PITChv2-dTAG-Puro (BRD4)

    Plasmid

    #91796

    Purpose

    PITCh dTAG donor vector for FKBP_F36V-2xHA-P2A-Puro knock-in into the C-terminus of the human BRD4 locus.

    Depositor

    James Bradner, Behnam Nabet

    Article

    Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

    Insert

    FKBP_F36V-2xHA-P2A-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Phenylalanine 36 to valine in FKBP12

    Promoter

    Promotorless

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. EGFP-Tau(WT)

    Plasmid

    #187023

    Purpose

    Expresses EGFP tagged human 2N4R tau (WT) in mammalian cells with CMV promoter

    Depositor

    George Bloom

    Article

    Rajbanshi et al Alzheimers Dement. 2023 Jan 12. doi: 10.

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pHIV-EGFP

    Plasmid

    #21373

    Purpose

    self-inactivating lentiviral plasmid for co-expression of your gene of interest and EGFP

    Depositor

    Zena Werb, Bryan Welm

    Article

    Welm et al Cell Stem Cell. 2008 Jan 10. 2(1):90-102

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG:GPI-GFP

    Plasmid

    #32601

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Rhee et al Genesis. 2006 Apr;44(4):202-18.

    Insert

    GPI-GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEGFP-N1-FUS/TLS-FLAGC

    Plasmid

    #60362

    Purpose

    Plasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.

    Depositor

    Patrick Calsou, Sébastien Britton

    Article

    Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCE-hSK

    Plasmid

    #41814

    Purpose

    Non-integrating (episomal) expression of human SOX2 and KLF4

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    SOX2, KLF4 (SOX2, KLF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCE-hUL

    Plasmid

    #41855

    Purpose

    Non-integrating (episomal) expression of human L-MYC and LIN28

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Insert

    L-MYC, LIN28 (MYCL, LIN28A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAAV-hSyn-PSDΔ1,2-FRBx3

    Plasmid

    #225827

    Purpose

    A component of SYNCit-K (Synapse-targeted chemically induced translocation of Kalirin-7)

    Depositor

    Haruo Kasai

    Article

    Sawada et al Science. 2024 Sep 27;385(6716):1459-1465

    Insert

    PSDΔ1,2-FRBx3
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCW57-MCS1-P2A-MCS2 (Blast)

    Plasmid

    #80921

    Purpose

    All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

    Depositor

    Adam Karpf

    Article

    Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Doxycycline inducible; p2a self cleav…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-CaMKII-PinkyCaMP

    Plasmid

    #232858

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-PARP1-3xFlag-WT

    Plasmid

    #111575

    Purpose

    Expresses full length PARP1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. lentiSAMv2

    Plasmid

    #75112

    Purpose

    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    May 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid

    #184905

    Purpose

    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    Presequence of Fo-ATPase subunit 9
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pEG BacMam

    Plasmid

    #160451

    Purpose

    vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

    Depositor

    Eric Gouaux

    Article

    Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge