78,024 results

1. pMSCV40-EF1a-hBCL10-SO

   Plasmid

   #75256

   Purpose

   retroviral expression vector for expression of StrepOne-Tagged human BCL10

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   Bcl10 (BCL10 Human)
   

   Use

   Retroviral

   Tags

   StrepOne Tag

   Expression

   Mammalian

   Promoter

   pMSCV-LTRs, EF1a

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXL-BacII-CAG-mCherry

   Plasmid

   #170519

   Purpose

   Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

   Insert

   CAG-mCherry
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40

   Plasmid

   #100848

   Purpose

   AAV expression of jRCaMP1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1 and AAV9

   Insert

   jRCaMP1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. 809 pcDNA3 GFP PTEN

   Plasmid

   #10759

   Depositor

   William Sellers

   Article

   Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

   Insert

   PTEN (PTEN Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. tet-pLKO shGATA6 puro

   Plasmid

   #72615

   Purpose

   Expresses an inducible short hairpin targeting human GATA6 sequence

   Depositor

   Kevin Janes

   Article

   Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

   Insert

   short hairpin GATA binding protein 6
   

   Use

   Lentiviral

   Tags

   None

   Expression

   Mammalian

   Promoter

   TRE Tight

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-BAF

   Plasmid

   #101772

   Purpose

   Expresses EGFP tagged BAF in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSico-shNKCC1

   Plasmid

   #83027

   Purpose

   expresses an shRNA against NKCC1

   Depositor

   Angelique Bordey

   Article

   Young et al J Neurosci. 2012 Sep 26;32(39):13630-8.

   Insert

   shNKCC1
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLentiRhoA2G

   Plasmid

   #40179

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Use

   Lentiviral

   Tags

   His-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPHK2 gRNA (BRDN0001145867)

   Plasmid

   #77094

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human SPHK2

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   SPHK2 (SPHK2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. GRB2-V5

    Plasmid

    #139631

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    GRB2 (GRB2 Human)
    

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

    Plasmid

    #105556

    Purpose

    AAV expression of EGFP from tMCK promoter

    Depositor

    James M. Wilson

    Has Service

    AAV9

    Insert

    EGFP
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    tMCK

    Available Since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PL452-hGH-polyA-PGK-neoR

    Plasmid

    #191681

    Purpose

    Donor plasmid backbone PGK-neoR

    Depositor

    Yuejun Chen

    Article

    Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

    Type

    Empty backbone

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMED10-bio-His

    Plasmid

    #51852

    Purpose

    Expresses full-length Transmembrane emp24 domain-containing protein precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    TMED10 (TMED10 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUW-tetO-3xFLAG-GATA2

    Plasmid

    #125600

    Purpose

    doxycycline-inducible expression of human GATA2 (3xFLAG tagged) in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Gomes et al Cell Rep. 2018 Dec 4;25(10):2821-2835.e7

    Insert

    GATA binding protein 2 (GATA2 Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG tag

    Expression

    Mammalian

    Promoter

    TRE-mCMV

    Available Since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCENPTΔC-dCas9-3xGFP

    Plasmid

    #198325

    Purpose

    Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

    Depositor

    Sarah McClelland

    Article

    Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

    Insert

    CENPTΔC (CENPT Human)
    

    Use

    Lentiviral

    Tags

    EGFP x 3 and dCas9

    Expression

    Mammalian

    Mutation

    truncation - encodes only amino acids 1-375

    Promoter

    CMV-TetO

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS-HA-hUBE1L

    Plasmid

    #12438

    Depositor

    Dong-Er Zhang

    Article

    Kim et al Mol Cell Biol. 2004 Nov . 24(21):9592-60

    Insert

    UBE1L (UBA7 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

17. Flag-FOXM1

    Plasmid

    #153136

    Purpose

    Expression of Flag tagged human FOXM1 in mammalian cells

    Depositor

    Stefan Koch

    Article

    Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

    Insert

    human FOXM1 (FOXM1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV IE94

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSG5-hPXR-K62Q

    Plasmid

    #67766

    Purpose

    Expression hPXR mutant K62Q in Mammalian Cells

    Depositor

    Sridhar Mani

    Insert

    human pregnane X receptor (PXR) (NR1I2 Human)
    

    Expression

    Mammalian

    Mutation

    lysine 62 to Glutamine

    Promoter

    SV40

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. Stat3-C Flag pRc/CMV

    Plasmid

    #8722

    Depositor

    Jim Darnell

    Article

    Bromberg et al Cell 1999 Aug 6;98(3):295-303.

    Insert

    Stat3-C (Stat3 Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A662C and N664C. Residues changed to Cys renders…

    Available Since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-KASH2

    Plasmid

    #187017

    Purpose

    Inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2
    

    Tags

    eGFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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