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4,457 results

Showing: 551 - 600 of 4457 results

  3. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

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  7. pYPQAT22

    Plasmid
    #223394
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for monocot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-hA3A-Y130F-SpRYD10A-UGI-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    May 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    223394-plasmid-map-sequence-id-474024

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  8. pYPQAT10

    Plasmid
    #223382
    Purpose
    T-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpRY-OsU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223382-plasmid-map-sequence-id-453506

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  13. pYPQAT19

    Plasmid
    #223391
    Purpose
    T-DNA vector for A3A/Y130F-CBE_V01 based C-to-T base editing for dicot plants; NG or NA PAM ; wide working window; A3A/Y130F-CBE_V01 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-hA3A-Y130F-SpRYD10A-UGI-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223391-plasmid-map-sequence-id-454180

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  18. pYPQ233-STU

    Plasmid
    #138111
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Article
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dLbCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available Since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138111-plasmid-map-sequence-id-272043

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  19. pGW_RUBY_EggCas9

    Plasmid
    #225990
    Purpose
    To clone entry vector with CRISPR guide RNA via Gateway cloning; it has an Egg cell-specific expression of Cas9 and the plant is selected by the red color due to constitutive expression of RUBY
    Depositor
    Imran Siddiqi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Oct. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    225990-plasmid-map-sequence-id-448019

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  20. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available Since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62204-plasmid-map-sequence-id-101817

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  21. nCas9-PPE

    Plasmid
    #140445
    Purpose
    For plant prime editing in wheat plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1
    Available Since
    May 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140445-plasmid-map-sequence-id-296171

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  26. pTC223

    Plasmid
    #70019
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 7
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Expression
    Plant
    Available Since
    Dec. 1, 2015
    Availability
    Academic Institutions and Nonprofits only
    70019-plasmid-map-sequence-id-124558

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  27. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91130-plasmid-map-sequence-id-182254

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  31. pYPQ220-Ultra

    Plasmid
    #176889
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing AsCas12a with M537R and F870L mutations, without promoter
    Depositor
    Yiping Qi
    Article
    Zhang et al Front Genome Ed. 2022 Jan 31;4:780238. d
    Insert
    AsCas12a
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Plant
    Available Since
    April 19, 2022
    Availability
    Academic Institutions and Nonprofits only
    176889-plasmid-map-sequence-id-350058

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  34. pCambia-loxKan

    Plasmid
    #199590
    Purpose
    basic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibility
    Depositor
    Andreas Stuermer
    Insert
    FRT-lox66-NosP-KanR-NosT-lox71-FRT
    Use
    Cre/Lox
    Expression
    Plant
    Promoter
    Nos
    Available Since
    July 5, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    199590-plasmid-map-sequence-id-399425

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  39. pdRBFC-NS1-YN

    Plasmid
    #74723
    Purpose
    Express C-terminal YN fused influenza A virus partial NS1 protein (27-236 nt) for dsRNA labeling
    Depositor
    Aiming Wang
    Article
    Cheng et al Virology. 2015 Nov;485:439-51. doi: 10.1
    Insert
    Influenza A virus NS1 protein, partial
    Use
    Double-stranded rna labeling
    Tags
    HA and YFP N-terminal half domain
    Expression
    Plant
    Promoter
    CaMV 35S
    Available Since
    May 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    74723-plasmid-map-sequence-id-146367

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  40. pYPQAT01

    Plasmid
    #223373
    Purpose
    T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by AtUBQ10 and the sgRNA was driven by AtU3 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223373-plasmid-map-sequence-id-454171

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  42. pDGB3omega1_KanR_BastaR

    Plasmid
    #186426
    Purpose
    optimisation of phosphinothricin (Basta) selection in plant cells; combines kanamycin resistance gene (nptII) and Basta resistance gene (bar)
    Depositor
    Lukas Fischer
    Article
    Kobercova et al Plant Cell Rep. 2023 Jan 7. doi: 10.1007
    Inserts
    KanR
    BastaR
    Use
    Synthetic Biology; Binary vector for escherichia …
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    CaMV 35S and Pnos
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    186426-plasmid-map-sequence-id-369066

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Showing: 551 - 600 of 4457 results