4,457 results
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Plasmid#101082PurposeGA biosensor expression plasmidDepositorInsertnlsGPS1-NR
TagscMycExpressionPlantMutationAtGAI E51K E54R, AtGID1C S114A F115APromoterp16 (AT3G60245)Available SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAK-EL-03
Plasmid#125762PurposeEndlinker for tRNA-gRNA system when using only three guidesDepositorInsertEndlinker
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pORFE0010
Plasmid#112074Purpose3xmVenus module CTDepositorInsert3xmVenus
ExpressionPlantMutationSer to Thr at amino acid 63 and Phe to Leu at pos…Available SinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB405
Plasmid#68670PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)
Plasmid#160627PurposeTU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domainsDepositorInsertP35s-MS2:TV-Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBAV150
Plasmid#79764Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneUseTetrExpressionPlantPromoterDEXAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRETgc-2in1-NC
Plasmid#105121PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmCherry and mEGFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLCS101
Plasmid#209355PurposeGateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter for N-terminal mVenus fusion protein.DepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRW004-SM-destination-proUBQ10-Cas9
Plasmid#104438PurposeDestination vector expressing plant-codon-optimized Cas9 under UBQ10 promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene free plants;DepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoterUBQ10Available SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSB116 - pL2_pSB90_fLUC-I_P19
Plasmid#123191Purposebinary plant vector for transient TBSV P19 and fLUC (with intron) expressionDepositorInsert2x35S::P19::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseExpressionPlantAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAXY0007
Plasmid#205576PurposeNpuDnaE(C)-Glucos(232-500)DepositorInsertNpuDnaE(C)-Glucosyltransferase
ExpressionPlantPromoter35SAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAXY0006
Plasmid#205575PurposeGlucos(1-231)-NpuDnaE(N)DepositorInsertsGlucosyltransferase and NpuDnaE(N)_Arabidopsis-codon-OP
CYP76AD1-P2A-DODA
ExpressionPlantPromoter35SAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB402
Plasmid#68667PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-ZmAXIG1-6
Plasmid#197711PurposePhytobrick (MoClo) Level 0 PartDepositorInsertZmAXIG1 promoter
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-NF-L3-NF-L1-dMb2Cas12a
Plasmid#177686PurposeGateway entry clone (attL1 & attR5) for a single chain FokI-dMb2Cas12a genome editingDepositorInsert2xFokI-dMb2Cas12a
UseCRISPR; Gateway compatible 2xfoki-dmb2cas12a entr…TagsNLSExpressionPlantAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH66
Plasmid#131765PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized, version 2):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized, version 2):NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:Mla1
Plasmid#132355PurposeExpression of the barley Mla1 NLR from the Zea mays ubiquitin promotorDepositorInsertHvMla1
ExpressionPlantPromoterpZmUBQAvailable SinceDec. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:AVRa1
Plasmid#132356PurposeExpression of the Blumeria graminis f. sp. hordei effector AVRa1 ∆SP from the Zea mays ubiquitin promotorDepositorInsertAVRa1 ∆SP with stop codon
ExpressionPlantPromoterpZmUBQAvailable SinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRETtv-2in1-NC
Plasmid#105122PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmTRQ2 and mVenusExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB202
Plasmid#68593PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZA72
Plasmid#174783PurposeIn planta gene expressionDepositorInsertGG_XopJ4, Kmr plant selection marker
ExpressionPlantMutationWT + STOP codonAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT1-24X-NOG(CmYLCV)
Plasmid#202014PurposeT-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the CmYLCV promoter.DepositorInsertsdCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1
TagsGB1 and sfGFPExpressionPlantPromoterCmYLCVAvailable SinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-WDV-Rep-RepA-9
Plasmid#197716PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV Rep/RepA proteins: native ORFs
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-WDV-LS-L-11
Plasmid#197719PurposePhytobrick (MoClo) Level 0 PartDepositorInsertWDV LSL in c-sense with GG expansion to v-sense ORF position
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDGE161
Plasmid#79453Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIDepositorAvailable SinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_223
Plasmid#91117PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: WDVDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGrDL_SP
Plasmid#83204Purposereceives miRNA target sequences for testing using the dual luciferase assay systemDepositorInsertSalI/PstI cloning sites
ExpressionPlantAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 P35S:PhiC31 integrase-RDF:T35S (GB2893)
Plasmid#160633PurposeTU for the constitutive expression of the PhiC31 integrase, C-terminally fused with its reversion factor (RDF)DepositorInsertPhiC31-RDF
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC6
Plasmid#104780PurposepSC218UG-At Ubiquitin10:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDGE668
Plasmid#153233PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantAvailable SinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LBJJ382
Plasmid#117518PurposeAlso known as LBJJ429. PCR template for sgRNA-EF with polyT, 67bp or 192bp terminatorDepositorInsertBpiI:ACTA:pU6-26:sgRNA-EF:t192bp:TTAC:BpiI
UseCRISPRExpressionPlantPromoterAtU6-26Available SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFPNL1
Plasmid#61651PurposeGateway vector for generating nucleus-targeted fluorescent protein fusions in fungiDepositorInsertmonomeric red fluorescent protein
ExpressionPlantAvailable SinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFH102
Plasmid#128207PurposesgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)DepositorInsertsgRNA backbone for SaCas9, combine with AtU6-26 promoter module (pFH35)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB118 - pL2_pSB90_fLUC-I_HcPro
Plasmid#123193Purposebinary plant vector for transient TEV HcPro and fLUC (with intron) expressionDepositorInsert2x35S::HcPro::tNOS 2x35S::fLUC-I::tNOS
UseLuciferaseExpressionPlantAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSS02:cyt-Peredox-mCherry_DS
Plasmid#161747Purposecytosolic expression of fluorescent NADH/NAD+ biosensor Peredox-mCherry in plantsDepositorInsertcyt-Peredox-mCherry_DS
ExpressionPlantMutationamino acid substitution D115S in first T-Rex doma…PromoterUbiquitin 10Available SinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantPromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-SlUbi-6
Plasmid#197709PurposePhytobrick (MoClo) Level 0 PartDepositorInsertSlUbi promoter
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-SlUbi-11
Plasmid#197773PurposePhytobrick (MoClo) Level 0 PartDepositorInsertSlUbi terminator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF005-sgRNA-shuffle-in
Plasmid#104441PurposeProvide and shuffle a cassette of AtU6:sgRNA-transRNA into SM-destination vectors (pRW006 and pRW004) with golden gate cloning strategy. Work together with pEF004.DepositorInsertsgRNA-transRNA transcription by U6 promoter
UseCRISPRExpressionBacterial and PlantAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only