4,306 results

1. pAK006

   Plasmid

   #128219

   Purpose

   improved sgRNA backbone for tRNA-gRNA array, Position 6

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for tRNA-gRNA array, Position 6
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGWB440

   Plasmid

   #74826

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eYFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pNC-TRV2

   Plasmid

   #193402

   Purpose

   Nimble cloning-compatible vector for virus-induced gene silencing

   Depositor

   Pu Yan

   Article

   Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYPQ284 (Mb2Cas12a)

   Plasmid

   #138116

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   Mb2Cas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BM00369

   Plasmid

   #155338

   Purpose

   Binary vector for expression of PULSE system (under the control of CaMV35S promoter) controlling GUS. It constitutively expresses dsRed and a plant selection cassette.

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   RB_KanR_Tnos-NLS-PIF6-E-P35S_Tnos-dsRed-PUbi10_Tnos-NLS-VP16-PhyB-P35S_Tnos-EL222-NLS-SRDX-P35S_T35S-GUS-POpto_LB
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGem-nHyPer:cAPX

   Plasmid

   #84736

   Purpose

   Simultaneous expresion of stromal HyPer2 and cytosolic Ascorbate peroxidase (APX) in plant cells

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Inserts

   APX1 (APX1 Mustard Weed)
   HyPer2 targeted to nucleus
   

   Use

   Tags

   NLS-SV40

   Expression

   Plant

   Mutation

   Promoter

   pFMV/p35S

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUB-cSypHer

   Plasmid

   #84733

   Purpose

   Expresses SypHer in plant cells-biosensor for pH

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

   Insert

   SypHer
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

   Available since

   July 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pE3c

   Plasmid

   #17463

   Purpose

   modified pENTR vector for C-terminal 6x MYC tag fusion with your gene of interest

   Depositor

   Giovanna Benvenuto

   Article

   Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

   Type

   Empty backbone

   Use

   Modified gateway entry vector

   Tags

   6xMyc

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   Promoter

   Available since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCLV3Cas-LT0

   Plasmid

   #210548

   Purpose

   Binary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT0 reporter

   Depositor

   Ortrun Mittelsten Scheid

   Article

   Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

   Insert

   CLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT0::mCherrySSM
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. LT0

    Plasmid

    #210551

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT0 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT0::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LT1

    Plasmid

    #210552

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT1 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT1::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LT2

    Plasmid

    #210553

    Purpose

    Binary vector expressing the Lineage Tracing system. No Cas9 control expression cassette. LT2 reporter

    Depositor

    Ortrun Mittelsten Scheid

    Article

    Dona et al Plant J. 2023 Jul 5. doi: 10.1111/tpj.16

    Insert

    pU6::AAVS1gRNA::LT2::mCherrySSM
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMB-NUCr

    Plasmid

    #61169

    Purpose

    Fluorescent reporter for nucleus NLS-mCherry-GUS expressed under MtBCP1 promoter

    Depositor

    Maria Harrison

    Article

    Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

    Insert

    NLS-mCherry-GUS
    

    Use

    Tags

    mCherry

    Expression

    Plant

    Mutation

    Promoter

    MtBCP1

    Available since

    Feb. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDGB3_alpha2: Pnos:ER:lexABD:GAL4AD:T35s-OplexA:mini35S:phi31:Tnos-Pnos:GR:LacIBD:Gal4AD:Tnos-OplacI:mini35S:RDF:Tnos (GB1679)

    Plasmid

    #160619

    Purpose

    Module for estradiol-inducible expression of the PhiC31 integrase gene and dexamethasone -inducible expression of PhiC31 phage recombination directionality factor (RDF) gene.

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

    Insert

    ERLexABDGal4AD / PhiC31 / GRLacIBDGal4AD / RDF
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    P35S

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PEP112E

    Plasmid

    #97398

    Purpose

    binary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Mitochondria target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGWB523

    Plasmid

    #74865

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAK002

    Plasmid

    #128215

    Purpose

    improved sgRNA backbone for tRNA-gRNA array, Position 2

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    improved sgRNA backbone for tRNA-gRNA array, Position 2
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWB507

    Plasmid

    #74849

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pYPQ230-RVR

    Plasmid

    #108861

    Purpose

    LbCpf1 Gateway entry plasmid with G548R, K554V and Y558R mutations

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Insert

    LbCpf1-RVR (LbCpf1 with G548R, K554V and Y558R mutations)
    

    Use

    CRISPR; Gateway compatible cpf1 entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    G548R, K554V and Y558R, Cpf1 is rice codon optimi…

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMC-1-ZmPLTP-6

    Plasmid

    #197713

    Purpose

    Phytobrick (MoClo) Level 0 Part

    Depositor

    Daniel Voytas

    Article

    Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

    Insert

    ZmPLTP promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGG-GFP-PM-TurboID

    Plasmid

    #209402

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    cTP-3XHA-TurboID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pFH6_new

    Plasmid

    #105866

    Purpose

    more efficient version of pFH6; Subcloning of any sgRNA via BbsI site

    Depositor

    Andreas Weber

    Article

    Hahn et al Bio Protoc. 2017 Jul 5;7(13):e2384. doi:

    Insert

    U6-26p::sgRNA scaffold
    

    Use

    CRISPR and Synthetic Biology; Subcloning

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGWB423

    Plasmid

    #74817

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGG-PM-TurboID

    Plasmid

    #209395

    Purpose

    Transiently expressing TurboID fused with a plasma menbrane targeting peptide in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMpGWB331

    Plasmid

    #68659

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGem-sHyper:sAPX

    Plasmid

    #84738

    Purpose

    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

    Inserts

    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    

    Use

    Tags

    nuclear localisation signals from the coding sequ…

    Expression

    Plant

    Mutation

    Promoter

    pFMV/p35S

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSB137 - pL2_pSB90_pNOS::rLUC-I::tNOS_tOCS::fLUC-I::pSTR1

    Plasmid

    #123194

    Purpose

    binary plant vector for transient dual luciferase (rLUC & fLUC with introns) expression

    Depositor

    Erin Cram, Carolyn Lee-Parsons

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    pNOS::rLUC-I::tNOS tOCS::fLUC-I::pSTR1
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. P525

    Plasmid

    #139739

    Purpose

    Individual component of Caffeic Acid biosynthesis pathway, hpaB

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    hpaB
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JAB2296

    Plasmid

    #190365

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

    Depositor

    Jose Dinneny, Jennifer Brophy

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JAB2121

    Plasmid

    #190343

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (empty vector control)

    Depositor

    Jose Dinneny, Jennifer Brophy

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMRMTK-clo6

    Plasmid

    #203957

    Purpose

    Cloning plasmid containing the kanamycin resistance gene insert

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Kanamycin Resistance Gene
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMDC32B-AtTAS1c-D2-B/c

    Plasmid

    #137884

    Purpose

    Plant expression vector (2x35S) for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor into position downstream 3'D1[+]

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Insert

    AtTAS1c-D2-B/c
    

    Use

    Tags

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    2x35S

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMP118

    Plasmid

    #207947

    Purpose

    GreenBraid destination vector pGBY1 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31I

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRAB18::LUC

    Plasmid

    #141168

    Purpose

    Luminescent reporter for abscisic acid signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtRAB18 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtRAB18, AT5G66400

    Available since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFRETgc-2in1-CN

    Plasmid

    #105118

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Use

    Tags

    mCherry and mEGFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMpGWB227

    Plasmid

    #68618

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. L2-AA007

    Plasmid

    #185750

    Purpose

    Vector to introduce HygR and eGFP under control of p35S into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.

    Depositor

    Peter Szoevenyi

    Article

    Frangedakis et al New Phytol. 2021 Jun 2. doi: 10.1111/nph

    Inserts

    HygR2
    eGFP
    

    Use

    Tags

    membrane localization tag Lti6b

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. AatII-pRPS5A-PTP-3Xflag-rbc L 294 Right_G1397 C-ABE

    Plasmid

    #189652

    Purpose

    rbc L 294 Right_G1397 C-ABE

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Plants. 2022 Dec 1. doi: 10.1038/s41

    Insert

    TALE and DddAtox and TadA 8e
    

    Use

    TALEN

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. 35S:YFPc-RabG3b-DN

    Plasmid

    #102374

    Purpose

    fluorescent tagging. Plant expression of YFPc-RabG3b-DN

    Depositor

    Silke Robatzek

    Insert

    RabG3B (RABG3B Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Plant

    Mutation

    T22N

    Promoter

    35S

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. 35S:YFPc-RabG3b-WT

    Plasmid

    #102373

    Purpose

    fluorescent tagging. Plant expression of YFPc-RabG3b-WT

    Depositor

    Silke Robatzek

    Insert

    RabG3B (RABG3B Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge