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Plasmid#153344PurposeMobius Assembly cloning and expression vector for plants with high-copy number origin of replicationDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only
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LLP752_L2pV1_AND_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192396PurposeTo act as a 2-input state of the AND gate.DepositorInsertAct2::FRT-OCS-FRT::B3RT-OCS-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP747_L2pV1_OR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192391PurposeTo act as a 2-input state of the OR gate.DepositorInsertAct2::FRT-B3RT-OCS-FRT-B3RT::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantMutationPromoterAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-ABI2-YFPn-1
Plasmid#102383Purposesplit YFP. Plant expression of ABI2-YFPn-1DepositorInsertABI2 (ABI2 Mustard Weed)
UseTagsYFPnExpressionPlantMutationPromoter35SAvailable sinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMM100
Plasmid#127225PurposeAtU6::sgRNA targeting PDSDepositorInsertsgRNA
UseTagsExpressionPlantMutationcodon optimized CDSsPromoterAvailable sinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
lvl0 HSPt
Plasmid#153378PurposePlant terminatorDepositorInsertHSP terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL1-Citrine:NOSt
Plasmid#175563PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-1DepositorInsertGOXL1
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)
Plasmid#160616PurposeTU for the constitutive expression of Streptomyces phage PhiC31 integrase.DepositorInsertPhiC31
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [D1_n-1] (GB1208)
Plasmid#75409PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [D1_n-1]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [D1_n-1]
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9_6
Plasmid#91169PurposeCas9 only expression, Cas9 Type: TaCas9, Plant Selection: PvUbi2:barDepositorInsertTaCas9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-eCFP
Plasmid#183155PurposePlant transient expression vector containing eCFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eCFPDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aa3.8
Plasmid#136376PurposeGateway entry clone for AaCas12b sgRNA expression under ZmUbi promoter with ribozyme processing; sgRNA scaffold 3.8 is usedDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPSup2
Plasmid#149418PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted about 1.4 kb upstream of the minimal promoter.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX033
Plasmid#126251PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf06112g01006.1
UseTagsHAExpressionPlantMutationPromoterCaMV 35SAvailable sinceJuly 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGMV-gSWEET4
Plasmid#112796PurposeUniversal BeYDV-derived vector with gRNAs targeting the VviSWEET4 geneDepositorInsertgRNA pair targeting VviSWEET4
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB407
Plasmid#74801PurposeGateway cloning compatible binary vector for C-terminal fusion with 6xHis (no promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_160
Plasmid#91206PurposeTransformation, Type: non-T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCBL101gg
Plasmid#199735PurposeGolden Gate recipient vector for transgene overexpressionDepositorInsertsacB
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLggCDS
Plasmid#199737PurposeGolden Gate entry vector for gene of interest cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCBLggTerm
Plasmid#199738PurposeGolden Gate entry vector for terminator cloningDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH51
Plasmid#128213Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with OsU3 promoter module (pFH38)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH23
Plasmid#128180PurposeLevel1 ZmUBIp::SpCas9 (wheat codon optimized):NOStDepositorInsertZmUBIp::SpCas9 (wheat codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH53
Plasmid#128182PurposeLevel1 2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3AtDepositorInsert2x35Sp::SpCas9 (Arabidopsis codon optimized):pea3At
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB Pnos:Luciferase:Tnos (GB0112)
Plasmid#68217PurposeTranscriptional unit for Firefly Luciferase Protein plant expression driven by the Nos promoterDepositorInsertLuc
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEE390
Plasmid#149280PurposeT-DNA encoding TRV2 with gRNA targeting NbPDS3DepositorInsertp35S:TRV2_NbPDS3sgRNA:tNos
UseTagsExpressionPlantMutationPromoter35SAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCk1
Plasmid#136695PurposeAcceptor plasmid for odd level position 1, lacZ blue-white screening. Part of the OpenPlant toolkit.DepositorInsertlacZ
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
cp-DdCBE G1333-N
Plasmid#174556PurposeChloroplast-targeting DdCBE (split DddA, G1333-N) Golden Gate destination vectorDepositorInsertDdCBE G1333-N
UseTagsHAExpressionPlantMutationPromoterPcUbiAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25I
Plasmid#91146PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationD10A, H840APromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
5aa SunTag VP64 EVD
Plasmid#115483PurposeCRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_omega2
Plasmid#68239PurposeGoldenBraid level 1 Omega plant expression vectorDepositorInsertempty vector
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P3_AtU626Ter26
Plasmid#191771PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL11197
Plasmid#191784PurposeSpCas9 expression in plantsDepositorInsertAtUbi10::Cas9 +13 introns-T-E9
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10
Plasmid#197965PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRTagsExpressionPlantMutationPromoterAtU6-26 and pUBQ10Available sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAGM55351
Plasmid#169092PurposeMoClo level 1 35S promoter-BvADHα-Nos terminator transcription unitDepositorInsert35S promoter-BvADHα-Nos terminator
UseMoclo cloning vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_10A
Plasmid#91207PurposeDirect Cloning, Type: non-T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: noneDepositorInsert35S:AtCas9 + AtU6:gRNA
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM168
Plasmid#84093Purposetarget miRNA168 for destruction in Arabidopsis thalianaDepositorInsertSTTM168
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM160
Plasmid#84085Purposetarget miRNA160 for destruction in Arabidopsis thalianaDepositorInsertSTTM160
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-TBS-11
Plasmid#197689PurposePhytobrick (MoClo) Level 0 PartDepositorInsertTBS enhancer-blocking insulator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-CmYLCV-4
Plasmid#197694PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCmYLCV promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-EGFP-9
Plasmid#197733PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-6-GFP-P2A-NptII-9
Plasmid#197741PurposePhytobrick (MoClo) Level 0 PartDepositorInsertEGFP-P2A-NptII bicistronic CDS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-9-35S-11
Plasmid#197759PurposePhytobrick (MoClo) Level 0 PartDepositorInsert35S terminator
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:dCas9:Tnos (GB1191)
Plasmid#68223PurposeTranscriptional unit for human codon optimized with mutated (D10A, H840A) and inactivated catalytic domains Cas9 protein plant expression driven by the 35S promoterDepositorInsertdCas9
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removed; human codon optimis…Promoter35SAvailable sinceMarch 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFH58
Plasmid#128184PurposeLevel1 ZmUBIp::SaCas9 (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::SaCas9 (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
lvl0 ACT7p
Plasmid#153371PurposePlant promoterDepositorInsertACT7 promoter
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
L2-AA012
Plasmid#185751PurposeVector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
UseTagsmembrane localization tag Lti6bExpressionPlantMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM173
Plasmid#84101Purposetarget miRNA173 for destruction in Arabidopsis thalianaDepositorInsertSTTM173
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXSE901B
Plasmid#91713PurposeCRISPR/Cas9-mediated base editing in dicots. Contains Cas9(D10A) under CaMV 35S promoter and empty gRNA scaffold, Bar resistanceDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NC
Plasmid#105123PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneUseTagsmVenus and tagRFPExpressionPlantMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only