4,441 results
-
Plasmid#109327PurposeGateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS driven by egg cell-specific promoterDepositorInsertzCas9
UseCRISPR; Gateway compatible zcas9 entry cloneTags3x FLAG, NLS and NLSExpressionPlantMutationZea mays codon-optimized Cas9PromoterPromoter EC1.2 enhancer fused to EC1.1 promoterAvailable SinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJG363
Plasmid#91183PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG367
Plasmid#91185PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_H840A+gNt_R2+gNt_F2+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG370
Plasmid#91187PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)DepositorInsertnAtCas9_D10A+gRNActrl+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJG382
Plasmid#91189PurposeBeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)DepositorInsertnAtCas9_D10A+gNt_R2+gNt_F2+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFH22
Plasmid#125808PurposeLevel0 xCas9 3.7DepositorInsertxCas9 3.7
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID
Plasmid#209393PurposeTransiently expressing TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
ExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-TurboID-GFP-PTS1
Plasmid#209406PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a peroxisomal targeting signal type I (PTS1) in plantaDepositorInsert3XHA-TurboID-PTS1
TagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
JAB2098
Plasmid#190340PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_pro35S-AmtRO-2XPhlFO::GFPint-ADHt
ExpressionPlantAvailable SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pORFE0011
Plasmid#112075PurposepcUBI promoter module Pro+5UDepositorInsertpcUBI promoter
ExpressionPlantMutationBsaI and BpiI restriction sites have been remove…Available SinceAug. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB419
Plasmid#74813PurposeGateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1139T10C
Plasmid#210838PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
ExpressionPlantAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMP119
Plasmid#207949PurposeGreenBraid destination vector pGBY2 - Eco31I - PaqCI -sfGFP- PaqCI - Eco31IDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-vCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10
Plasmid#197966PurposeT-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by CmYLCVp, flanked by ribozymes.DepositorInsertspco-vCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterCmYLCVp and pUBQ10Available SinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB522
Plasmid#74864PurposeGateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141-ZmUbi-RZ-Aac
Plasmid#129685Purpose(Empty backbone) Gateway entry clone with sgRNA under ZmUbi promoterDepositorInsertEmpty backbone
UseCRISPRExpressionPlantAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [M1_n-1] (GB1210)
Plasmid#75411PurposetRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_n-1]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (2-part multiplexing)DepositorInserttRNA-gRNA position [M1_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ11p
Plasmid#153365PurposePlant promoterDepositorInsertUBQ11 promoter
ExpressionPlantAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL12015
Plasmid#117506PurposeAtUBI10 promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pUBI10:AATG:BsaI
ExpressionPlantPromoterAtUBI10Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGB Tnos:NptII:Pnos (GB0226)
Plasmid#68212PurposeTranscriptional unit for kanamycin resistance gene plant expressionDepositorInsertNptII
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterPnosAvailable SinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pORE303N_CEN12RRFuzzy3MYB
Plasmid#194441PurposeCRISPR, Cas9 driven by double 35S, nptII for plant selection, knockout: CEN12, RR, and Fuzzy3MYBDepositorInsertgRNA array targeting CEN1, RR, Fuzzy3MYB
UseCRISPRExpressionPlantPromoterMtU6Available SinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CTAG_mScarlet-I
Plasmid#136118PurposeFP for C-term fusion with CDS12DepositorInsertCTAG-mScarletI(noATG)
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
INCTbiosyn-pAct-AtINTBxb1
Plasmid#127536PurposePlasmid encodes A. thaliana codon optimized Integrase Bxb1.DepositorInsertIntegrase Bxb1 coding sequence codon optimized for A. thaliana expression.
ExpressionPlantAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUBN-paGFP-Dest
Plasmid#105108Purposefluorescent tagging in stably transformed plantsDepositorTypeEmpty backboneTagspaGFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941DR5-PacI-ath-STTM390
Plasmid#84117Purposetarget miRNA390 for destruction in Arabidopsis thalianaDepositorInsertSTTM390
ExpressionPlantPromoter2x35SAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2301y-tdtomato-dCas9_DegronDead_TPLN188
Plasmid#205409PurposedCas9 guided repressorDepositorInsertdCas9_DegronDead_TPLN188
ExpressionPlantAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:MS2:EDLL:Tnos (GB1738)
Plasmid#160622PurposeTU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain EDLLDepositorInsertP35s_MS2:EDLL_Tnos
ExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGS2.2 (FF573)
Plasmid#35248DepositorTypeEmpty backboneExpressionPlantPromoterCaMV 35SAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
MIGS5.1 (FF572)
Plasmid#35253DepositorTypeEmpty backboneExpressionPlantAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGREAT18
Plasmid#170906PurposeGoldenBraid Double Transcriptional Unit - pAtUBQ10_E-Luc_AtHSP18.2t+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpAtUBQ10Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
UBC1cer60G
Plasmid#161016Purposeubiquitously weakly expressed 60-meric GFP nanocageDepositorInsert60meric GFP nanocage
ExpressionPlantAvailable SinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
P366
Plasmid#139722PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, NpgaDepositorInsertpMOD_A''_pSlRBCS2:AsNpga:tAtuG7
UseLuciferaseExpressionPlantPromoterSlRBCS2Available SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH49
Plasmid#128211Purposeimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)DepositorInsertimproved sgRNA backbone for tRNA-gRNA array, Position 1, combine with AtU6-26 promoter module (pFH34)
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY200
Plasmid#69946PurposeAgrobacterium-based transformation vectorDepositorTypeEmpty backboneExpressionPlantAvailable SinceOct. 13, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDIRECT_21B
Plasmid#91129PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
UseCRISPRExpressionPlantMutationD10A, H840AAvailable SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BCJJ180
Plasmid#117516PurposeCas9_4 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_4:GCTT:BsaI
UseCRISPRTags3xFLAG and NLSExpressionPlantAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
mUAV
Plasmid#153362PurposeConverts DNA sequences into PhytobricksDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_MCS_blp
Plasmid#159710PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneExpressionPlantAvailable SinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNapin-eGFP
Plasmid#127797PurposeNapin Promoter driven eGFP expressionDepositorTypeEmpty backboneExpressionPlantAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pILL5-NLS-3xmCitrine
Plasmid#192525PurposeFluorescent reporter for plant responses to bacterial infectionDepositorInsertIAA-leu-resistant-like5 (ILL5 Mustard Weed)
Tags3xmCitrine and NLSExpressionPlantPromoterILL5Available SinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAMBIA-FLS2prom::FLS2-3xmyc
Plasmid#86158PurposeFLS2 expressionDepositorAvailable SinceFeb. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM858a/b
Plasmid#84177Purposetarget miRNA858 for destruction in Arabidopsis thalianaDepositorInsertSTTM858a/b
ExpressionPlantPromoter2x35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 BlpR
Plasmid#153390PurposeConfers resistance to bialaphos or phosphinothricin (Glufosinate)DepositorInsertBlpR
ExpressionPlantAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9_1
Plasmid#91156PurposeCas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9
UseCRISPRExpressionPlantAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pICSL90016
Plasmid#117514PurposeCas9_2 Golden Gate Level 0DepositorInsertBsaI:AATG:Cas9_2:GCTT:BsaI
UseCRISPRTagsNLS_SV40ExpressionPlantAvailable SinceNov. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
p33Fn
Plasmid#158155PurposeExpression of crRNA array and FnCas12a using one Pol II promoter. For plant genome editing.DepositorTypeEmpty backboneUseCRISPRExpressionPlantMutationRice codon optimizedAvailable SinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMP107
Plasmid#207925PurposePremade plasma membrane marker, pUBQ10:PiP1;4:PmScarlet-I:tHSP18.2M, for braidingDepositorTypeEmpty backboneExpressionPlantAvailable SinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFH45
Plasmid#125810PurposeLevel0 nSpCas9-ABE7.10 base changer, human codon optimizedDepositorInsertnSpCas9-ABE7.10 base changer, human codon optimized
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-v2
Plasmid#138119PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R, N563R and K569R mutations, without promoterDepositorInsertMb2Cas12a v2
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172R, N563R and K569RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pCmYLCV911
Plasmid#170878PurposePhytobrick - pCmYLCV911DepositorInsertpCmYLCV911
ExpressionPlantAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only