4,625 results
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Plasmid#191784PurposeSpCas9 expression in plantsDepositorInsertAtUbi10::Cas9 +13 introns-T-E9
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pICSL11023
Plasmid#117542PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-h (no stop codon)(pICSL90005) + YFP (pICSL50005)+ Nos (pICH41421)
ExpressionPlantPromoter2x35S_OMEGA(pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10
Plasmid#197965PurposeT-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.DepositorInsertspco-nCasphi-2
AtPDS3 gRNA10
UseCRISPRExpressionPlantPromoterAtU6-26 and pUBQ10Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTC208
Plasmid#70017PurposeTALENs targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based onTomato Yellow Leaf Curl VirusDepositorInsertNuclease (TALENs) + Donor + GVR
UseTALENExpressionPlantAvailable SinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAGM55351
Plasmid#169092PurposeMoClo level 1 35S promoter-BvADHα-Nos terminator transcription unitDepositorInsert35S promoter-BvADHα-Nos terminator
UseMoclo cloning vectorExpressionPlantAvailable SinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
MIGS3.1 (FF537)
Plasmid#35249DepositorTypeEmpty backboneExpressionPlantPromoterCaMV 35SAvailable SinceMay 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYPQ284-RVR
Plasmid#138117PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoterDepositorInsertMb2Cas12a RVR variant
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationN563R, K569V and N573RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-TBS-11
Plasmid#197689PurposePhytobrick (MoClo) Level 0 PartDepositorInsertTBS enhancer-blocking insulator
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMC-1-CmYLCV-4
Plasmid#197694PurposePhytobrick (MoClo) Level 0 PartDepositorInsertCmYLCV promoter
ExpressionPlantAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
L2-AA012
Plasmid#185751PurposeVector to introduce eGFP under control of pAaEF1a into the genome of the hornwort Anthoceros agrestis via Agrobacterium mediated transformation. eGFP contains a cell membrane localization tag.DepositorInsertsHygR2
eGFP
Tagsmembrane localization tag Lti6bExpressionPlantAvailable SinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-NC
Plasmid#105123PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmVenus and tagRFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRETvr-2in1-CN
Plasmid#105120PurposeFRET/FLIM analysis in plants (stable or transient transformation)DepositorTypeEmpty backboneTagsmVenus and tagRFPExpressionPlantAvailable SinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
L1P79
Plasmid#195940PurposeLevel 1 pGATA23 reporter : pGATA23-mScarlet-NLS-tRBCSDepositorInsertpGATA23-mScarlet-NLS-tRBCS
ExpressionPlantAvailable SinceMarch 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
LLP675_L2_pV01_1I_vox-OCS-Rluc_TCTP_Vika_lacZ
Plasmid#192427PurposeTo act as the on state for testing of the alt Vika recombinase.DepositorInsertAct2::vox-OCS-vox::Rluc
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
LLP772_L2pV1_NOR_B3RT_FRT_Rluc_2IBF_lacZ
Plasmid#192432PurposeTo act as a 2-input state of the NOR gate.DepositorInsertAct2::FRT-B3RT-Rluc-B3RT-FRT
UseSynthetic BiologyTagsPESTExpressionPlantAvailable SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH80
Plasmid#128191PurposeLevel1 ZmUBIp::xCas9 3.7:NOStDepositorInsertZmUBIp::xCas9 3.7:NOSt
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:EDLL:tNos (GB1190)
Plasmid#75404PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the EDLL Transcriptional ActivatorDepositorInsertdCas:EDLL
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLXMA L2A
Plasmid#153360PurposeExpression vector for plants with medium-copy number origin of replicationDepositorTypeEmpty backboneExpressionPlantAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHMWGFPR
Plasmid#64396PurposeHigh molecular weight glutenin gene promoter driving GFP expressionDepositorInsertHigh molecular weight glutenin gene promoter
TagsGFPExpressionPlantPromoterHigh molecular weight glutenin gene promoterAvailable SinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131-STU-As
Plasmid#138094PurposeGolden Gate entry vector for 1st crRNA cloning with AsCas12a crRNA scaffold flanked by HH and HDV ribozymesDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB409
Plasmid#68674PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CERK1 D441V-YFPc-1
Plasmid#102403Purposesplit YFP. Plant expression of CERK1 D441V-YFPc-1DepositorAvailable SinceOct. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-Lyk5-YFPn-1
Plasmid#102389Purposesplit YFP. Plant expression of Lyk5-YFPn-1DepositorAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-CBL2-YFPc-1
Plasmid#102394Purposesplit YFP. Plant expression of CBL2-YFPc-1DepositorAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB415
Plasmid#68680PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged Citrine (Citri…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
lvl0 UBQ5t
Plasmid#153383PurposePlant terminatorDepositorInsertUBQ5 terminator
ExpressionPlantAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJG488
Plasmid#91196PurposeWDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)DepositorInsertnTaCas9_H840A+gUbi8+gUbi1+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2151
Plasmid#141300PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable SinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB332
Plasmid#68660PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterMpHSP17.8A1 promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM395
Plasmid#84121Purposetarget miRNA395 for destruction in Arabidopsis thalianaDepositorInsertSTTM395
ExpressionPlantPromoter2x35SAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEE389
Plasmid#149287PurposeT-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_TrunFT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWB14-LYX161
Plasmid#126379PurposeWith a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.DepositorInsertNiben101Scf04395g00017.1
TagsHAExpressionPlantPromoterCaMV 35SAvailable SinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSC43
Plasmid#104817PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoterDepositorInsertGlyma.04g057400
UseCRISPRExpressionPlantAvailable SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC44
Plasmid#104818PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma.04g057400
UseCRISPRExpressionPlantAvailable SinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
cARA12-mRFP
Plasmid#181961PurposeARA12 protein tagged with mRFP, under control of TBA2 promoterDepositorAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
secCitrine
Plasmid#181964PurposeBasic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoterDepositorInsertsignal peptide sequence from basic chitinase
TagsCitrine YFPExpressionPlantPromoter1.5 kb MUM4 promoterAvailable SinceMarch 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEE387
Plasmid#149285PurposeT-DNA encoding TRV2 with FT augmented gRNA targeting NbAGDepositorInsertp35S:TRV2_NbAGsgRNA1_FT:tNos
ExpressionPlantPromoter35SAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRN168
Plasmid#79668PurposeDsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.DepositorTypeEmpty backboneUseReporter plasmidTagsDsRED-monomer (1–168)/RN168ExpressionPlantPromoterCaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGZ12.0501
Plasmid#141164PurposeTcTT2 in Ti plasmid binary vector pGZ12.0501 (KF871320.1)DepositorInsertTcTTC
ExpressionPlantPromoterE12-omegaAvailable SinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG20_FLAG_Hyg
Plasmid#159713PurposepGREEN-based cloning construct for expression in plantaDepositorTypeEmpty backboneTagsFLAGExpressionPlantAvailable SinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3_alpha2 35:PhyB:VP16 (GB2745)
Plasmid#160630PurposeTU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switchDepositorInsertPhyBVP16
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM398
Plasmid#84124Purposetarget miRNA398 for destruction in Arabidopsis thalianaDepositorInsertSTTM398
ExpressionPlantPromoter2x35SAvailable SinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM394
Plasmid#84204PurposeDisturb the expression of miR394 in riceDepositorInsertmiR394
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTaCas9-D10A_5
Plasmid#91170PurposeCas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt IIDepositorInsertTaCas9_D10A (nickase)
UseCRISPRExpressionPlantMutationD10AAvailable SinceJuly 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM166
Plasmid#84197PurposeDisturb the expression of miR166 in riceDepositorInsertmiR166
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBGC155-MXMT
Plasmid#79818PurposeGFP(S65T)-based BiFC vector for plants (Positive control: MXMT)DepositorInsertCaMXMT1
UseReporter plasmidTagsGC155/GFP (155–239)ExpressionPlantPromoterCaMV 35S promoter and CaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBGN155
Plasmid#74992PurposeGFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plantsDepositorTypeEmpty backboneUseReporter plasmidTagsGFP (1–155)/GN155ExpressionPlantPromoterCaMV35SAvailable SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB125
Plasmid#68579PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292-D570A
Plasmid#129679PurposeGateway entry clone for CRISPR-Cas12b systemsDepositorInsertAaCas12b
UseCRISPR; Gateway compatible aacas12b entry cloneExpressionPlantMutationD570A; AaCas12b is rice codon optimizedAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pABD1031
Plasmid#68004PurposeProtein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in plantaDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
TagseYFPExpressionPlantPromoter2X Cauliflower Mosaic Virus 35S promoterAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only