4,307 results

1. pAM-35s-CERK1 D441V-YFPc-1

   Plasmid

   #102403

   Purpose

   split YFP. Plant expression of CERK1 D441V-YFPc-1

   Depositor

   Silke Robatzek

   Insert

   CERK1 (CERK1 Mustard Weed)
   

   Use

   Tags

   YFPc

   Expression

   Plant

   Mutation

   D441V

   Promoter

   35S

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pAM-35s-Lyk5-YFPn-1

   Plasmid

   #102389

   Purpose

   split YFP. Plant expression of Lyk5-YFPn-1

   Depositor

   Silke Robatzek

   Insert

   LYK5 (LYK5 Mustard Weed)
   

   Use

   Tags

   YFPn

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ141C2.0

   Plasmid

   #99905

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU6 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU6

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pER8 cHA-AIP2

   Plasmid

   #118687

   Purpose

   Plant inducible expression

   Depositor

   Kristiina Himanen

   Article

   Pavicic et al Front Plant Sci. 2017 Mar 28;8:416. doi:

   Insert

   AIP2 (AT5G20910 Mustard Weed)
   

   Use

   Tags

   HA

   Expression

   Plant

   Mutation

   Promoter

   LexA

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. P16ΔS:S10-(MCS)-3xHA

   Plasmid

   #108183

   Purpose

   Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   P16ΔS

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDIRECT_22D

   Plasmid

   #91136

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAM-35s-CBL2-YFPc-1

   Plasmid

   #102394

   Purpose

   split YFP. Plant expression of CBL2-YFPc-1

   Depositor

   Silke Robatzek

   Insert

   CBL2 (CBL2 Mustard Weed)
   

   Use

   Tags

   YFPc

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBRC169

   Plasmid

   #79670

   Purpose

   DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

   Depositor

   Masamitsu Wada, Yutaka Kodama

   Article

   Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

   Type

   Empty backbone

   Use

   Reporter plasmid

   Tags

   DsRED-monomer (169–225)/RC169

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pH7m34GW_RcCHAD-mCherry

   Plasmid

   #127784

   Purpose

   RcCHAD-mCherry expression in plants

   Depositor

   Michael Hothorn

   Article

   Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

   Insert

   Ubi10p:RcCHAD-mCherry
   

   Use

   Tags

   mCherry

   Expression

   Plant

   Mutation

   Promoter

   Ubiquitin10 promoter + 5' UTR

   Available since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGWB307

    Plasmid

    #68635

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. tRNA-gRNA position [n] (GB1207)

    Plasmid

    #75408

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    tRNA-gRNA position [n]
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTX168

    Plasmid

    #89257

    Purpose

    Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

    Depositor

    Daniel Voytas

    Article

    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBCC155-MXMT

    Plasmid

    #79816

    Purpose

    CFP-based BiFC vector for plants (Positive control: MXMT)

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Insert

    CaMXMT1
    

    Use

    Reporter plasmid

    Tags

    CC155/CFP (155–239)

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S promoter and CaMV35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSC4

    Plasmid

    #91182

    Purpose

    T-DNA vector for targeted deletion of 58kb region in Medicago truncatula (tRNA array of 6 gRNAs under the control of CmYLCV promoter)

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    gRNAs targeting 58kb region in medicago truncatula
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGWB14-LYX161

    Plasmid

    #126379

    Purpose

    With a Nicotiana benthamiana transcription factor in PGWB14 backbone, for Agrobacterium mediated transient or stable expression corresponding transcription factor fused with a C-terminal HA tag.

    Depositor

    Silin Zhong

    Insert

    Niben101Scf04395g00017.1
    

    Use

    Tags

    HA

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSC43

    Plasmid

    #104817

    Purpose

    CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoter

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.04g057400
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSC44

    Plasmid

    #104818

    Purpose

    CRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Insert

    Glyma.04g057400
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. cARA12-mRFP

    Plasmid

    #181961

    Purpose

    ARA12 protein tagged with mRFP, under control of TBA2 promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    ARA12 (ARA12 Mustard Weed)
    

    Use

    Tags

    mRFP

    Expression

    Plant

    Mutation

    Promoter

    TBA2

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. secCitrine

    Plasmid

    #181964

    Purpose

    Basic chitinase signal peptide fused to Citrine, under control of MUM4_1.5kb promoter

    Depositor

    George Haughn

    Article

    Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

    Insert

    signal peptide sequence from basic chitinase
    

    Use

    Tags

    Citrine YFP

    Expression

    Plant

    Mutation

    Promoter

    1.5 kb MUM4 promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSB96 - pL2_pSB90_2x35S::fLUC-I::tNOS

    Plasmid

    #123188

    Purpose

    binary plant vector for transient fLUC (with intron) expression

    Depositor

    Carolyn Lee-Parsons, Erin Cram

    Article

    Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

    Insert

    2x35S::fLUC-I::tNOS and VirGN54D in the vector backbone
    

    Use

    Luciferase

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGZ12.0501

    Plasmid

    #141164

    Purpose

    TcTT2 in Ti plasmid binary vector pGZ12.0501 (KF871320.1)

    Depositor

    Mark Guiltinan

    Article

    Florez et al BMC Plant Biol. 2015 May 16;15:121. doi:

    Insert

    TcTTC
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    E12-omega

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. BCJJ340

    Plasmid

    #117502

    Purpose

    UBI10:Cas9:E9 Golden Gate Level 1 Position 2

    Depositor

    Jonathan D Jones

    Article

    Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

    Insert

    BpiI:GCAA:UBI10:Cas9-3(intron):E9:ACTA:BpiI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtUBI10

    Available since

    Oct. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pG20_FLAG_Hyg

    Plasmid

    #159713

    Purpose

    pGREEN-based cloning construct for expression in planta

    Depositor

    Hans-Henning Kunz

    Article

    Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGB2alpha2 35s:hCas9:tNos (GB0639)

    Plasmid

    #68222

    Purpose

    Transcriptional unit for (human codon optimized) Cas9 plant expression driven by the 35S promoter

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    hCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    35S

    Available since

    March 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pYPQ141-ZmUbi-RZ-Aa3.8.5

    Plasmid

    #158407

    Purpose

    Express single gRNA with Aa3.8.5 scaffold (with four MS2 binding sites) under ZmUbi promoter

    Depositor

    Yiping Qi

    Article

    Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Maize ubiquitin 1

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Osa-pCAMBIA-1301-STTM394

    Plasmid

    #84204

    Purpose

    Disturb the expression of miR394 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR394
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFGC5941-PacI-ath-STTM5026

    Plasmid

    #84192

    Purpose

    target miRNA5026 for destruction in Arabidopsis thaliana

    Depositor

    Guiliang Tang

    Article

    Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

    Insert

    STTM5026
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MIGS3.2 (FF574)

    Plasmid

    #35250

    Depositor

    Detlef Weigel

    Article

    de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. lvl0 G10-90p

    Plasmid

    #153370

    Purpose

    Synthetic promoter for plant expression

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    G10-90 promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFH52

    Plasmid

    #128181

    Purpose

    Level1 2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    2x35Sp::SpCas9 (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pMpGWB231

    Plasmid

    #68622

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Emerald Luc fused with PEST sequence [ELuc(PEST)]

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pTRANS_102

    Plasmid

    #91200

    Purpose

    Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMpGWB409

    Plasmid

    #68674

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    3xFLAG

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYPQ284-RVR

    Plasmid

    #138117

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V and N573R mutations, without promoter

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Insert

    Mb2Cas12a RVR variant
    

    Use

    CRISPR; Gateway compatible cas12a entry clone

    Tags

    NLS

    Expression

    Plant

    Mutation

    N563R, K569V and N573R

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFH85

    Plasmid

    #128198

    Purpose

    classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for SpCas9, combine with TaU3 promoter module (pFH33)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMpGWB436

    Plasmid

    #68701

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pHMWGFPR

    Plasmid

    #64396

    Purpose

    High molecular weight glutenin gene promoter driving GFP expression

    Depositor

    Claudia Vickers

    Article

    Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

    Insert

    High molecular weight glutenin gene promoter
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    High molecular weight glutenin gene promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

38. pDIRECT_21D

    Plasmid

    #91131

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pTRANS_263

    Plasmid

    #91127

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pABD1031

    Plasmid

    #68004

    Purpose

    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta

    Depositor

    Bruce Downie

    Article

    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

    Insert

    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    

    Use

    Tags

    eYFP

    Expression

    Plant

    Mutation

    Promoter

    2X Cauliflower Mosaic Virus 35S promoter

    Available since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only