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1,782 results

Showing: 61 - 80 of 1782 results

  1. pJW2098

    Plasmid
    #163094
    Purpose
    CRISPR repair template: add insertion site specific homology arms by PCR before insertion
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    30aa linker::mScarlet (dpi)::AID*::3xFLAG::10aa linker
    Use
    CRISPR
    Expression
    Worm
    Mutation
    Silent mutations to remove piRNA sites
    Available Since
    Jan. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163094-plasmid-map-sequence-id-320582

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  4. pDD346

    Plasmid
    #133311
    Purpose
    SapTrap mNG-C1 donor
    Depositor
    Daniel Dickinson
    Insert
    C. elegans codon-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans codon-optimized mNeonGreen
    Expression
    Worm
    Promoter
    None
    Available Since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    133311-plasmid-map-sequence-id-263341

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  5. pCC324

    Plasmid
    #110165
    Purpose
    miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    R61K change made in ERK-KTR CDS
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110165-plasmid-map-sequence-id-235338

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  8. pCC336

    Plasmid
    #110167
    Purpose
    miniMos vector expresses ERKKTR(S43E,T55E,T62E)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43E,T55E,T62E)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43E, T55E, T62E.…
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110167-plasmid-map-sequence-id-238139

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  9. pCC335

    Plasmid
    #110166
    Purpose
    miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains Neomycin resistance)
    Depositor
    Iva Greenwald
    Article
    de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.
    Insert
    ERK-KTR(S43A,T55A,T62A)-mClover-T2A-mCherry-H2B
    Use
    Minimos transposon
    Tags
    mCherry and mClover
    Expression
    Worm
    Mutation
    Phospho-acceptor sites mutated: S43A, T55A, T62A.…
    Promoter
    lin-31 promoter/enhancer
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    110166-plasmid-map-sequence-id-235342

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  10. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  15. pJM483

    Plasmid
    #52987
    Purpose
    A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor
    Depositor
    Ding Xue
    Article
    Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d
    Insert
    Lactaherin (Mfge8 Mouse)
    Tags
    GFP (w/ introns) and signal sequence
    Expression
    Worm
    Mutation
    deleted native signal sequence, deleted C-termina…
    Promoter
    hsp-16.41
    Available Since
    June 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    52987-plasmid-map-sequence-id-82563

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Showing: 61 - 80 of 1782 results