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Plasmid#154122Purposemec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vectorDepositorInsertmec-4p::rtetR-L-QFAD::act-4 '3
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEM246 - MCS mosTI unc-119 target
Plasmid#159821PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertMCS mosTI unc-119 target
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
Plasmid#159849PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD121
Plasmid#91833PurposeCas9 expression in C. elegansDepositorInsertCas9
UseCRISPRTagsExpressionWormMutationCodon optimized and with synthetic introns for C.…PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSR49
Plasmid#69259Purposephlh-8::tagBFP2DepositorInsertTagBFP2
UseCre/LoxTagsExpressionWormMutationcodon-optimzed index 1.0, TagBFP2 contains 2 sing…Promoterhlh-8Available sinceOct. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2
Plasmid#182358PurposePositive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.DepositorInsertGFP
UseTagsExpressionWormMutationPromoterPmex-5Available sinceMay 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNM3724
Plasmid#154112PurposetetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vectorDepositorInserttetO 7X::∆pes-10::GFP-C1::tbb-2 3'
UseRmceTagsExpressionWormMutationPromoternot applicableAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLGCV
Plasmid#49862PurposeExpression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).DepositorInsertfirefly luciferase luc+ fused to GFP (S65C)
UseTagsGFP (S65C)ExpressionWormMutationPromotersur-5Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
Plasmid#159857PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD378
Plasmid#91827PurposeCodon-optimized HaloTag donor for the SapTrap cloning systemDepositorInsertHaloTag-C1
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)
Plasmid#193853PurposePromoterless insertion vector for split HygR/mScarlet-I landing padDepositorInsert5' rps-0::HygR + 5' mScarlet-I
UseCRISPR and Cre/LoxTagsExpressionWormMutationPromoterrps-0pAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
ChrII_ttTi5605_loxP_myo2_neoR
Plasmid#194056PurposeDual-selection cassette plasmid for knocking-in at the ChrII_ttTi5605 of the C. elegans genomeDepositorInsertttTi5605
UseTagsExpressionWormMutationPromoterNoneAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEft-3::Cerulean-Venus::Tomm-7
Plasmid#68046PurposeUbiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.DepositorInsertpEft-3::Cerulean-Venus::Tomm-7
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWP018
Plasmid#198766PurposeDrives specific expression in the ADE, CEP, and PDE neuronsDepositorInsertPdat-1-GAL4-SK(DBD)-VP64
UseTagsExpressionWormMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKS235
Plasmid#191855PurposeNegative selection marker for MosSCIDepositorInsertpeel-1 toxic protein for negative selection against extrachromosomal array
UseTagsExpressionWormMutationPromoterhsp-16.41Available sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
ChrV_oxTi365_loxP_myo2_neoR
Plasmid#194057PurposeDual-selection cassette plasmid for knocking-in at the ChrV_oxTi365 of the C. elegans genomeDepositorInsertoxTi365
UseTagsExpressionWormMutationPromoterNoneAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
sgRNA with U6 promoter
Plasmid#48962Purposeto drive the sgRNA expression under a U6 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterU6Available sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2
Plasmid#182351PurposeGermline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDJ22 - pEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
Plasmid#159814PurposeOptimized enzyme for genome editing in C. elegansDepositorInsertpEXP[Psmu-2 | Cre Recombinase (PATCs) | gpd-2 tagRFP-T smu-1 UTR]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ108 - pENTR221 - [cbr-unc-119(+)]
Plasmid#200367Purposecbr-unc-119 rescue gene for use as co-injection marker.DepositorInsert[cbr-unc-119(+)]
UseTagsExpressionWormMutationPromoterAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
Pflp-18::NGL-1::spGFP11
Plasmid#65828PurposeFor trans-synaptic labelingDepositorInsertNLG-1 (nlg-1 Nematode)
UseTagsspGFP11ExpressionWormMutationN142SPromoterPflp-18Available sinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJW1839
Plasmid#154325PurposesgRNA Destination Vector for SapTrapDepositorInsertSapTrap sgRNA (F+E) vector, R07E5.16 U6 promoter
UseCRISPRTagsExpressionWormMutationF+E mutations in sgRNAPromoterAvailable sinceAug. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD288
Plasmid#137017PurposemNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertmNG-GLO^SEC^3xFlag + ccdB
UseCRISPR and Cre/LoxTags3xFlag and C. elegans GLO-optimized mNGExpressionWormMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BSP188
Plasmid#110917PurposeFor cloning by HR in yeast, Universal MosSCI compatible, has unc54 terminator clonedDepositorInsertunc54 Terminator
UseSynthetic Biology; Yeast homologous recombination…TagsExpressionWormMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJF6
Plasmid#139040PurposeMosSCI SapTrap assembly: Cassette 3 for 4-cassette systemDepositorInsertgfp (includes STOP codon, PATC introns)
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
TC358
Plasmid#164885PurposeTRE::GFP, inducible GFP expression when co-express with rtTADepositorInsertGFP
UseTagsExpressionWormMutationPromoterAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJH4169
Plasmid#200108PurposePrig-3 FRT let858 (stop) FRT snb-1::GFP unc-54 3' UTR C.elegans AVA and other neurons expression of snb GFPDepositorInsertsnb-1 (snb-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPrig-3Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHW449
Plasmid#198805PurposeDirect light-gated cation channel from Chlamydomonas reinhardtii that allows neuron deploarization through brief pulses of blue light.DepositorInsert15xUAS::hChR2(H134R)-YFP::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJH3626
Plasmid#179630PurposePacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFPDepositorInserttomm20::miniSOG (tomm-20 Nematode)
UseTagsRFPExpressionWormMutationPromoterPacr-2(s)Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHygCG2r
Plasmid#200074PurposehygR selection mNG marked rapid RMCE integration vectorDepositorTypeEmpty backboneUseRecombination mediated cassette exchangeTagsExpressionWormMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJRK261
Plasmid#173754PurposeCeLINC plasmidDepositorInsert(empty)::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHW581
Plasmid#198818PurposeIn vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kineticsDepositorInsert15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
UseTagsExpressionWormMutationPromoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJW2341
Plasmid#186950Purpose30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linkerDepositorInsert30aa linker::GFP-C1::IAA7 AID::3xFLAG::10aa linker
UseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Punc-4::NGL-1::spGFP1-10
Plasmid#65827PurposeFor trans-synaptic labelingDepositorInsertNLG-1 (nlg-1 Nematode)
UseTagsspGFP1-10ExpressionWormMutationPromoterPunc-4Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
XW12
Plasmid#65834PurposePquas-Δpes10-GFP-3'UTR-unc-54DepositorInsert5XQUAS-delta-pes-10
UseTagsGFPExpressionWormMutationPromoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSN444
Plasmid#108331PurposeExpresses Sat2 for Nourseothricin resistance selection of C. elegansDepositorInsertStreptothricin acetyl transferase 2
UseTagsExpressionWormMutationPromoterrps-27 promoterAvailable sinceAug. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)
Plasmid#107133PurposecGAL-N driver under the control of C. elegans hsp-16.41 promoterDepositorInsertNLS::cGAL(DBD)::gp41-1-N-intein
UseTagsExpressionWormMutationPromoterC. elegans hsp-16.41 promoterAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrII_ttTi5605.sgRNA
Plasmid#194058PurposesgRNA targeting ChrII_ttTi5605 locus of the C. elegans genomeDepositorInsertChrII_ttTi5605 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTK73.ChrV_oxTi365.gRNA
Plasmid#194052PurposesgRNA targeting ChrV_oxTi365 locus of the C. elegans genomeDepositorInsertChrV_oxTi365 sgRNA
UseTagsExpressionWormMutationPromoterU6Available sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only