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1,779 results

Showing: 241 - 280 of 1779 results

  1. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid
    #47550
    Purpose
    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele
    Depositor
    Bob Goldstein
    Article
    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm
    Inserts
    Cas9
    ttTi5605 sgRNA
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Worm
    Mutation
    Codon optimized and with synthetic introns for C.…
    Promoter
    U6 and eef-1A.1 (eft-3)
    Available Since
    Sept. 9, 2013
    Availability
    Academic Institutions and Nonprofits only
    47550-plasmid-map-sequence-id-67865

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  4. lis-1::L4440

    Plasmid
    #11336
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    RNAi
    Expression
    Worm
    Available Since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. pJK2020

    Plasmid
    #171932
    Purpose
    express MS2 coat protein (MCP) fused to superfolder GFP and histone subunit H2B fused to mCherry in an operon
    Depositor
    Judith Kimble
    Article
    Lee et al Dev Cell. 2019 Aug 19;50(4):426-435.e4.
    Insert
    Pmex-5::MS2 Coat Protein::linker::sfGFP::tbb-2 3'UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3'UTR
    Tags
    superfolder GFP, mCherry
    Expression
    Worm
    Promoter
    -
    Available Since
    July 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    171932-plasmid-map-sequence-id-337179

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  25. pCZGY2850

    Plasmid
    #87758
    Purpose
    Pcol-19-PH::miniSOG(Q103L), expression of enhanced mutant version of membrane targeted PH-miniSOG (Q103L) in epidermis.
    Depositor
    Andrew Chisholm
    Article
    Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10
    Insert
    Pcol-19-PH-miniSOG(Q103L)
    Tags
    PH
    Expression
    Worm
    Mutation
    Q103L
    Promoter
    Pcol-19
    Available Since
    March 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    87758-plasmid-map-sequence-id-174819

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  26. pCFJ1320

    Plasmid
    #84829
    Purpose
    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns
    Depositor
    Andrew Fire
    Article
    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10
    Insert
    C. elegans codon optimized GFP
    Expression
    Bacterial and Worm
    Promoter
    Peft-3
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    84829-plasmid-map-sequence-id-163669

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  29. pDD400

    Plasmid
    #137024
    Purpose
    SapTrap mNG-GLO donor
    Depositor
    Daniel Dickinson, Bob Goldstein
    Insert
    C. elegans GLO-optimized mNeonGreen
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized mNeonGreen
    Expression
    Worm
    Available Since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137024-plasmid-map-sequence-id-274031

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  32. pSLGCV

    Plasmid
    #49862
    Purpose
    Expression in Caenorhabditis elegans of the luc+ gene (Promega), fused in frame to GFP (S65C), under the sur-5 promoter (from plasmid pTG96, John Yochem and Min Han). Backbone pPD95.79 (Firelab).
    Depositor
    Anne Glover, Jonathan Pettitt
    Article
    Lagido et al BMC Physiol. 2008 Apr 2;8:7. doi: 10.118
    Insert
    firefly luciferase luc+ fused to GFP (S65C)
    Tags
    GFP (S65C)
    Expression
    Worm
    Promoter
    sur-5
    Available Since
    April 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    49862-plasmid-map-sequence-id-74359

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  34. pHT101-mCherry

    Plasmid
    #61021
    Purpose
    Expresses mCherry tagged fusion proteins in worms
    Depositor
    Casonya Johnson
    Type
    Empty backbone
    Tags
    mCherry
    Expression
    Worm
    Promoter
    lac
    Available Since
    Nov. 21, 2014
    Availability
    Academic Institutions and Nonprofits only
    61021-plasmid-map-sequence-id-98700

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Showing: 241 - 280 of 1779 results