8,265 results
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pREP3-TEV-NLS
Plasmid#21146DepositorInsertTEV protease (TEVgp1 tobacco etch virus)
UseS. pombeTags2xNLS, NLS, and mycExpressionYeastAvailable SinceJuly 6, 2009AvailabilityAcademic Institutions and Nonprofits only -
pGWKS12
Plasmid#139419PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker.DepositorInsertSuperfolder GFP
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGWKS4
Plasmid#139411PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker.DepositorInsertmCherry
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBHM2678
Plasmid#241723PurposeConstruction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BLE marker for selection on phleomycinDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBHM2680
Plasmid#241725PurposeConstruction of fused marker-sgRNA inserts for Cryptococcus neoformans genome editing, contains BSR marker for selection on blasticidin SDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceSept. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pV1393:SpRY
Plasmid#172189PurposeGenome editing of C. albicansDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEVA1
Plasmid#58953PurposeContains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.DepositorInsertVAR1u, VAR1 recoded for expression from the nucleus
UseYeast ecoli shuttle vectorTagspreCox4 mitochondrial matrix targeting signalExpressionYeastMutationSynthetic gene in standard genetic codePromoterADH1Available SinceAug. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVTU_260_BirA
Plasmid#63682PurposeEncodes E. coli biotin ligase gene (BirA) for expression in yeast cellsDepositorInsertBirA
Tags6X Histidine and TEV cleavage siteExpressionYeastPromoterADH1/yeastAvailable SinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-eGFP
Plasmid#126717Purposeyeast plasmid overexpressing eGFPDepositorInsertegfp
ExpressionYeastPromoterGAPAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
NM R2E2 GFP
Plasmid#1208DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutation2 additional copies of second oligopeptide repeat…Available SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pV1200
Plasmid#111429PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - integrates at ENO1 - Recyclable for serial mutagenesis (Sap2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303-PADH1-AFB2
Plasmid#99530PurposeS. cerevisiae expression of F-box protein AFB2 under control of the ADH1 promoter, HIS3 marker (for use with the AID degron system)DepositorAvailable SinceSept. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS160 CRISPEY HDV-HDV NotI
Plasmid#114453PurposeYeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozymeDepositorTypeEmpty backboneUseCRISPR; Cen/arsExpressionYeastPromoterScGAL7pAvailable SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIp ADE2-HIS3
Plasmid#122904PurposeADE2-HIS3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
ZJOM75
Plasmid#133649PurposeIntegration of ANP1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAV0328
Plasmid#133489PurposeExpression of nucleo-cytoplasmic mCherryDepositorInsertpUra4(AfeI)-p(act1)-mCherry-terminator(tdh1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS416 GAL1pro-YFP
Plasmid#224875PurposeLow copy plasmid with GAL1-driven YFP for C-terminal tagging (no start codon)DepositorTypeEmpty backboneTagsYFPExpressionYeastPromoterGAL1Available SinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB6633
Plasmid#106162PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6677 into Yarrowia lipolytica chromosomal location IntE_1, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
ExpressionYeastPromoter5 XREs+ minimal Cytochrome CAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIYC04
Plasmid#64741PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS426 Gal TDP43
Plasmid#27466DepositorInsertTDP-43 (TARDBP Human)
ExpressionYeastAvailable SinceJan. 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKmK.P3
Plasmid#125036PurposeStorage plasmid for K.marxianus promoter; type 2 partDepositorInsertENO1 promoter
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGWKS2
Plasmid#139410PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker.DepositorInsertGFP S65T
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2
Plasmid#85119Purposeyeast expression of EEF2DepositorAvailable SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminator
Plasmid#158585PurposeExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiaeDepositorInsertmembrane localization signal-tagBFP-wrmScarlet11
TagstagBFPExpressionYeastMutationGGGS linkerPromoterTEFAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT1
Plasmid#98299PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of TEF1/TEF2 promoterDepositorInsertPTEF2-ERG20-TRPL3-PTEF1-AcNES1-TRPL41B
ExpressionYeastAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pL-RPB1
Plasmid#10981DepositorAvailable SinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pVW296
Plasmid#139496PurposeExpression vector for the PP7-mCherry protein in S. cerevisiaeDepositorInsertpADH1 PP7∆FG-mCherry tCYC1
ExpressionYeastMutation∆FGPromoterpADH1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pLAT1_Cas9
Plasmid#104908PurposehCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2795
Plasmid#63650PurposepCfB2795 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)
Plasmid#86418PurposeONM/ER split GFP1-10DepositorInsertNOP1pr-GFP1-10-Scs2TM
TagsGFP1-10ExpressionYeastPromoterNOP1Available SinceFeb. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p2UGFP
Plasmid#1195DepositorInsertGFP w/ stop codon
ExpressionYeastAvailable SinceJune 23, 2005AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pRM102
Plasmid#44525DepositorInsertP(GAL10)-HHT2 P(GAL1)-HHF2
ExpressionYeastPromoterGAL 10Available SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
p426*-SaSgH
Plasmid#136994PurposeHelper plasmid for SaCas9 gRNA cloning and expressionDepositorTypeEmpty backboneExpressionYeastPromoterSNR52Available SinceMay 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pKmK.C4
Plasmid#125062PurposeStorage plasmid for K.marxianus ARSDepositorInsertKmARS2
UseSynthetic BiologyExpressionYeastAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTK094-LM-AMA002.0
Plasmid#171366PurposeEntry Vector compatible, fungal replicating, shuttle backbone vector from the Fungal Modular Cloning ToolKit.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRG206
Plasmid#64527PurposeIntegrative E. coli/S. cerevisiae shuttle vectorDepositorTypeEmpty backboneExpressionYeastAvailable SinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-tdTomato-SpHis5
Plasmid#44640PurposeYeast tagging vector to create gene fusion with tdTomato with SpHis5 selectionDepositorInserttdTomato
ExpressionYeastAvailable SinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-GBP-RFP-kanMX6
Plasmid#105160PurposeYeast gene targetingDepositorInsertGBP-RFP-kanMX6
TagsGBP-RFPExpressionBacterial and YeastAvailable SinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENO1-BFP-NAT
Plasmid#50046PurposeExpression of BFP in Candida albicansDepositorInsertBFP
ExpressionYeastPromoterpENO1Available SinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
TDH3-STE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)LexAVP16-tCYC
Plasmid#135465PurposeMembrane-anchored TF used for yeast directed evolution of TEV proteaseDepositorInsertSTE2Δ(301-431)-BFP-CIBN-MKII-eLOV-TEVcs(ENLYFQ/M)-LexAVP16
TagsBFP, flagExpressionYeastPromoterTDH3Available SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG2J
Plasmid#102882PurposeExpresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.DepositorInsertsOsTIR1
mGFP-AID
TagsAID(71-114)ExpressionYeastPromoterADH1 and TEF1Available SinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKmK.T3
Plasmid#125055PurposeStorage plasmid for K.marxianus terminator; type 4 partDepositorInsertKMXK_A3020 terminator
UseSynthetic BiologyExpressionYeastAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSJ1679 (pRS315-NOP1pr-GFP11-PUS1)
Plasmid#86414Purposenuclear split GFP11 reporter without mCherryDepositorInsertNOP1pr-GFP11-PUS1
TagsGFP11 and mCherryExpressionYeastPromoterNOP1prAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only
