8,350 results
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Plasmid#26845DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only
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pRY003
Plasmid#81043PurposeHO endonuclease expressionDepositorInsertHO
ExpressionYeastPromoterendogenousAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
EC2_4_dCas9_VPR_sgRNA
Plasmid#163709PurposeYeast low copy plasmid with dCas9, sgRNA expression cassette and VPR activation domainsDepositorInsertsdCas9
VPR
ExpressionYeastMutationPoint mutations D10A and H840APromoterScTEF1Available SinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRP991_PACT1(-1-520)-LexA-ER-Gal4AD
Plasmid#58438PurposeExpresses LexA-ER-Gal4AD from yeast cellsDepositorInsertLexA-ER-Gal4AD
ExpressionYeastPromoterAct1 promoter (-520;1)Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Ube2v1
Plasmid#31429DepositorAvailable SinceOct. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2
Plasmid#122876PurposeADE2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Ade2 drive
Plasmid#89577PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Ade2 locusDepositorInsertAde2 gene drive
UseSynthetic BiologyExpressionYeastAvailable SinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP218
Plasmid#26831DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
416Gal-FUS-P525L-YFP
Plasmid#29628DepositorAvailable SinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR
Plasmid#24378DepositorInsertpADH1 yeCherry::p150yeGFP-DHFR
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pM-TagRFP657
Plasmid#176563PurposeExpression of TagRFP657 for auxotrophic selection in the absence of methionineDepositorInsertTagRFP657
ExpressionYeastPromoterTDH1(GAP)Available SinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFM699
Plasmid#74636Purposealias pFA6a-mCherry-natNT2 - vector for C-terminal tagging of yeast genes with mCherry and nourseothricin selection markerDepositorInsertmCherry
ExpressionYeastPromoternoneAvailable SinceJune 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN5
Plasmid#180286PurposeNatR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - NatR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
EGFP-FKBP(M)x4
Plasmid#85003PurposeExpresses EGFP-FKBP(M)x4 in yeastDepositorInsertEGFP-FKBP(M)x4
ExpressionYeastAvailable SinceDec. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSJ1726 (pFA6-NATMX-CDC42pr-KAR2SS-GFP1-10)
Plasmid#86421PurposeN-terminal PCR tagging cassette for yeast codon optimized split GFP1-10 with CDC42 promoter and KAR2 signal sequenceDepositorInsertCDC42pr-KAR2SS-GFP1-10
TagsGFP1-10ExpressionYeastAvailable SinceFeb. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pYES260-sc.eEF2 K509A
Plasmid#85120Purposeyeast expression of mutant EEF2DepositorTags6xHisExpressionYeastMutationK509APromoterGAL1Available SinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-GAL-Hyg
Plasmid#232091PurposeYeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertSpCas9 and Ec86 reverse transcriptase
UseCRISPRExpressionYeastMutationEc86 reverse transcriptase is codon optimized for…PromoterGAL1-GAL10Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat
Plasmid#232097PurposeYeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with NatMX selectionDepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
p416 25Q GAL
Plasmid#1185DepositorAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMTKM07
Plasmid#233479PurposeSpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and URA selection.DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
p413_Csy4NLS
Plasmid#103139PurposeExpresses Csy4-NLS under TEF promoterDepositorInsertCsy4
TagsNLSExpressionYeastPromoterTEFAvailable SinceJan. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFA6a-6xGLY-His-tag-HIS3MX6
Plasmid#20762DepositorTypeEmpty backboneUsePcr-based c-terminal taggingExpressionYeastAvailable SinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastPromoteryeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP418
Plasmid#26843DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits only -
pJB166
Plasmid#86998PurposeCRISPR/Cas9 in fission yeast using fluoride selectionDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03R122K/R126K
Plasmid#28005DepositorInsertAT1.03R122K/R126K
TagsmVenus and mseCFPExpressionYeastMutationR122K, R126KAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pNKY51
Plasmid#14839DepositorInserthisG-URA3-hisG
ExpressionYeastAvailable SinceApril 27, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFS561
Plasmid#242861PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetS promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS562
Plasmid#242862PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW1 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW1 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS564
Plasmid#242864PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW3 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW3 promoterAvailable SinceSept. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFS565
Plasmid#242865PurposeThis plasmid will be used to replace the promoter of a gene with the PenotetSW4 promoter at its endogenous locusDepositorInsertsTetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRS413-TEF1pr-MFA-NFAPoptim
Plasmid#221095PurposeFAP insert for N-terminally tagging genes of interest (optimized FAP for yeast expression + 2xMYC tag) + MFA1 signal sequence to help target constructs to the ERDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGI3EM18
Plasmid#135484PurposeAgrobacterium T-DNA with SpunH2Bpr driving hph-tdTomatoDepositorInsertSpunH2Bpr hph-tdTomato ADH1t
TagstdTomatoExpressionBacterial and YeastPromoterSpunH2BprAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PCUP1-DuDre-Atg8-404
Plasmid#69201PurposeRFP-Atg8 (PAS marker) with improved propertyDepositorInsertDuDre-Atg8
TagsDsRed Express 2ExpressionYeastAvailable SinceSept. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMJA181
Plasmid#128539PurposeExpresses anti-Prunus dulcis scFv Ab+BirA tagDepositorInsertscFv Ab clone PD1F6 plus BirA tag
ExpressionYeastPromoterAOX1Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoSuperfolderGFP-CaURA3
Plasmid#44873PurposeYeast tagging vector to create gene fusion with yeast optimized SuperfolderGFP with CaUra3 selectionDepositorInsertyoSuperfolderGFP
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-LEU
Plasmid#160006PurposeLow-copy expression vector for K. marxianus, leucine selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM20
Plasmid#133644PurposeIntegration of PEX1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast peroxisome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV125
Plasmid#63191PurposepRS425 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceOct. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG305GAL-ccdB
Plasmid#14137DepositorTypeEmpty backboneUseGateway CloningExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-EGFP-ccdB
Plasmid#14314DepositorTypeEmpty backboneUseGateway CloningTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJH2970
Plasmid#100954PurposeCas9 driven by PGK1 promoter. gRNA can be cloned into the BplI sites. HIS3MX markerDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-Nb(VHHGFP4)
Plasmid#198412PurposeExpress mAID-Nb(VHHGFP4) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (VHHGFP4)
ExpressionYeastPromoterADH1 promoterAvailable SinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MmSphk1
Plasmid#118599PurposeExpresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorInsertMus musculus sphingosine kinase 1
TagsFLAGExpressionYeastPromoterGAL1, 10Available SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMJA179
Plasmid#128537PurposeExpresses anti-Prunus dulcis scFv AbDepositorInsertscFv Ab clone PD1F6
ExpressionYeastPromoterAOX1Available SinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWR76
Plasmid#202776Purposeepisomal fluorescent reporter plasmid for S. stipitisDepositorInsertsTEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
coHPH
ACT1 terminator (S. stipitis)
TDH3 promoter (S. stipitis)
coCherry
ADH2 terminator (S. stipitis)
ARS from S. stipitis chromosome 1
ExpressionYeastAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only