8,010 results

1. 3413 pBF339 Bcl-XL

   Plasmid

   #8773

   Depositor

   Stanley Korsmeyer

   Article

   Gross et al Mol Cell Biol 2000 May;20(9):3125-36.

   Insert

   Bcl-XL (Bcl2l1 Mouse)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

2. pYZ293

   Plasmid

   #102687

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRG714_lexO-AscI_LexA-TF_URA3MX

   Plasmid

   #154822

   Purpose

   Inducible expression of AscI endonuclease in S. cerevisiae

   Depositor

   Lorraine Symington

   Article

   Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

   Inserts

   AscI endonuclease
   LexA-ER-B112
   

   Use

   Tags

   FLAG and NLS

   Expression

   Yeast

   Mutation

   Promoter

   ACT1 and lexO_4-CYC1_core

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-ATG8(414)/GFP-AUT7(414)

   Plasmid

   #49424

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.

   Depositor

   Daniel Klionsky

   Article

   Abeliovich et al Mol Biol Cell. 2003 Feb;14(2):477-90.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pEZY45

   Plasmid

   #18704

   Purpose

   pJG4-5 converted Gateway Destination vector for yeast two-hybrid prey vector

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Two-hybrid prey vector

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pK_P-GAP-ACC1*

   Plasmid

   #126740

   Purpose

   yeast plasmid overexpressing ACC1 from Komagataella phaffii

   Depositor

   Menghao Cai

   Article

   Liu et al Metab Eng. 2019 Jul;54:275-284. doi: 10.

   Insert

   acc1*
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   T3452G

   Promoter

   GAP

   Available since

   Oct. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pI3-MTU-DO-G418

   Plasmid

   #160023

   Purpose

   Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

   Depositor

   John Morrissey

   Article

   Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

   Type

   Empty backbone

   Use

   Kluyveromyces marxianus

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   n/a

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRS416-PHO5-GFP-Osh1

   Plasmid

   #58835

   Purpose

   Expresses GFP-Tagged Osh1 in yeast

   Depositor

   Tim Levine

   Article

   Loewen et al EMBO J. 2003 May 1;22(9):2025-35.

   Insert

   Osh1 (SWH1, OSH1 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   PHO5

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

9. ZJOM136

   Plasmid

   #133660

   Purpose

   Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
   

   Use

   Tags

   mTagBFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHES795

    Plasmid

    #87943

    Purpose

    Constitutively expresses estradiol-inducible synthetic transcription factor in yeast. It activates transcription of promoters containing Zif268 binding sites.

    Depositor

    Hana El-Samad

    Article

    Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

    Insert

    Zif268 DBD - hER LBD - MSN2 AD (ESR1 Human, Synthetic, Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 (crippled)

    Available since

    Jan. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYM29-BirA*

    Plasmid

    #160291

    Purpose

    Used for C-terminal BirA* tagging of proteins in Saccharomyces cerevisiae; Selection marker: TRP1

    Depositor

    Martin Ott

    Article

    Salvatori et al Mol Cell. 2020 Feb 20;77(4):887-900.e5.

    Insert

    BirA*
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAWH-largeT

    Plasmid

    #133895

    Purpose

    Positive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pB

    Depositor

    Sebastian Maurer

    Article

    Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

    Insert

    LargeT
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Dec. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pFA6a-link-yECFP-Hygro

    Plasmid

    #44647

    Depositor

    Kurt Thorn

    Insert

    yEmCFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSM1959

    Plasmid

    #41837

    Depositor

    Susan Michaelis

    Article

    Metzger et al J Biol Chem. 2008 Nov 21;283(47):32302-1

    Insert

    SEC63-mRFP
    

    Use

    Tags

    mRFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pWS3867

    Plasmid

    #185744

    Purpose

    Sub-array backbone 3/4

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p426-SNR52p-gRNA.csr-1.Y-SUP4t

    Plasmid

    #68060

    Purpose

    gRNA for csr-1 locus in N. crassa

    Depositor

    Christian Hong

    Article

    Matsu-Ura et al Fungal Biol Biotechnol. 2015 Jul 15;2:4.

    Insert

    gRNA for csr-1 locus
    

    Use

    CRISPR; N. crassa, fungi

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMJ123: pRS426-His-MBP-SpuFz1_PSP1

    Plasmid

    #205256

    Purpose

    S. cerevisiae expression vector for SpuFz1 reprogrammed guide (targeting PSP1)

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    SpuFz1
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKmK.T1

    Plasmid

    #125053

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    INU1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p414ADH_Hsp82_CycTER

    Plasmid

    #61712

    Purpose

    yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDB4280

    Plasmid

    #98699

    Purpose

    A Cas9-encoding plasmid containing the 5' portion of the ura4 marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-ura4 system

    Depositor

    Li-Lin Du

    Article

    Zhang et al G3 (Bethesda). 2018 Apr 27. pii: g3.118.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRS316-4M5.3

    Plasmid

    #45831

    Depositor

    Dane Wittrup

    Insert

    4M5.3
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMTU-DO-URA

    Plasmid

    #160004

    Purpose

    Low-copy expression vector for K. marxianus, uracil selection

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    n/a

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRS712

    Plasmid

    #213041

    Purpose

    CRISPR activation based overexpression in Nakaseomyces (Candida) glabrata

    Depositor

    Rebecca Shapiro

    Article

    Maroc et al mSphere. 2024 Feb 28;9(2):e0076123. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAG416GPD-ccdB-EGFP

    Plasmid

    #14196

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 3xFLAG-dCas9/pTEF1p-CYC1t

    Plasmid

    #62190

    Purpose

    Expresses 3xFLAG-dCas9 in budding yeast for enChIP analysis to purify specific genomic regions of interest.

    Depositor

    Hodaka Fujii

    Article

    Fujii et al Biol Methods Protoc. 2022 Oct 17;7(1):bp

    Insert

    3xFLAG-dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    human codon-optimized, D10A, H840A

    Promoter

    TEF1 promoter

    Available since

    Feb. 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pDRF1 yeBFP2-T2A-mCherry

    Plasmid

    #219851

    Purpose

    equimolar expression of yeBFP2 and mCherry in budding yeast

    Depositor

    Bas Teusink

    Insert

    yeBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDRF1-GW yomTagBFP2-T2A-mCherry

    Plasmid

    #205493

    Purpose

    Expression of yomTagBFP2-T2A-mCherry in yeast

    Depositor

    Bas Teusink

    Insert

    yomTagBFP2-T2A-mCherry
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGBK CUP1 Empty

    Plasmid

    #169710

    Purpose

    Yeast two-hybrid vector using a copper inducible CUP1 promoter

    Depositor

    Bruce Hamilton

    Article

    Hernandez et al bioRxiv

    Type

    Empty backbone

    Use

    Tags

    GAL4 DNA Binding Domain and Myc

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    CUP1

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDRF1-GW yomCherry-T2A-mTurquoise2

    Plasmid

    #118447

    Purpose

    Produces equimolar expression of yeast codon-optimized mCherry and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    yomCherry-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pROS11

    Plasmid

    #107925

    Purpose

    amdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y, gRNA-ADE2.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCTcon2-5INS_16

    Plasmid

    #102292

    Purpose

    Yeast Surface Display of 5Ins_16 scFV

    Depositor

    Sarel Fleishman

    Article

    Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

    Insert

    Insulin binding scFV
    

    Use

    Tags

    3x(G4S) Linker, G3S Linker, HA, and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUDE1093

    Plasmid

    #204227

    Purpose

    Episomal yeast plasmid expressing the Ercas12a

    Depositor

    Jean-Marc Daran

    Article

    Bennis et al FEMS Yeast Res. 2023 Jan 4;23:foad043. d

    Insert

    Eubacterium rectale cas12a (Ercas12a)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    codon optimized for S. cerevisiae

    Promoter

    Saccharomyces cerevisiae PGK1

    Available since

    Sept. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pMJA065

    Plasmid

    #128517

    Purpose

    Expresses Ber e 1 in Pichia

    Depositor

    Marcos Alcocer

    Article

    Alcocer et al J Mol Biol. 2002 Nov 15;324(1):165-75.

    Insert

    Ber e 1 a allergenic 2S albumin from Brazil nut
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. C-SWAT AID*-GFP osTIR1

    Plasmid

    #182514

    Purpose

    C-SWAT compatible vector for C-terminal tagging with AID*-GFP for auxin inducible gene knockdown. osTIR1 is flanked by SIR4 homology arms for integration.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    AID*
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFA6a-natCX

    Plasmid

    #73893

    Purpose

    fission yeast drug selection marker

    Depositor

    Kazunori Tomita

    Article

    Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

    Insert

    natCX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pIW447-CRISPR XYL2

    Plasmid

    #98906

    Purpose

    K. marxianus CRISPR Plasmid for XYL2 editing

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRS406-PHO5-GFP-hFAPP1 PH domain

    Plasmid

    #58723

    Purpose

    Expresses GFP-Tagged human FAPP1 PH domain in yeast

    Depositor

    Tim Levine

    Article

    Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

    Insert

    Four-phosphate-adaptor protein 1 PH domain (PLEKHA3 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pLSPR24

    Plasmid

    #217309

    Purpose

    Promoterless (SwaI Cutsite) mRuby3:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLSPR25

    Plasmid

    #217310

    Purpose

    Promoterless (SwaI Cutsite) mNeonGreen:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mNeonGreen

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLSPR30

    Plasmid

    #217315

    Purpose

    GAL7(p)-mRuby3:amdS fusion reporter

    Depositor

    James Fraser

    Article

    Phillips-Rose et al J Fungi (Basel). 2024 Aug 12;10(8):567.

    Insert

    Acetamidase
    

    Use

    Tags

    mRuby3

    Expression

    Yeast

    Mutation

    Promoter

    GAL7

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge