8,010 results
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Plasmid#217315PurposeGAL7(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR31
Plasmid#217316PurposeTEF1(p)-mRuby3:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmRuby3ExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR32
Plasmid#217317PurposeGAL7(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterGAL7Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLSPR33
Plasmid#217318PurposeTEF1(p)-mNeonGreen:amdS fusion reporterDepositorInsertAcetamidase
UseTagsmNeonGreenExpressionYeastMutationPromoterTEF1Available sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T-
Plasmid#161481PurposeNon targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Non targeting control NTC)
UseTagsExpressionYeastMutationPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pTCW012
Plasmid#83527PurposeCloning destination vector for phermone mediated expression from the FUS1J2 promoter and CYC1 terminatorDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpFUS1J2Available sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p413TEF-PKM1
Plasmid#34607DepositorInsertPKM1 (PKM Human)
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)
Plasmid#179079PurposeSp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
HO-Poly-HO
Plasmid#51661Purposeyeast plasmid for integration of target sequence at HO locusDepositorTypeEmpty backboneUseYeast integrationTagsExpressionYeastMutationPromoterAvailable sinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV0584
Plasmid#133471PurposeIntegration into fission yeast genome at ura4 locus with natMX selection cassetteDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP935
Plasmid#159302PurposeYeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCu-MCP-2yeGFP(405)
Plasmid#121419PurposeExpressing MCP-2yeGFPDepositorInsertCUP1p-MCP-2yeGFP
UseTagsExpressionYeastMutationPromoterCUP1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2374
Plasmid#127579PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Met15 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
FRP2372
Plasmid#127578PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Lys2 selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorTagsExpressionYeastMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDS257
Plasmid#34971PurposeExpresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.DepositorInsertMid2(SS/TM)-GFP-LOVpep
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGAPZ-STE2-1D4
Plasmid#133423PurposeYeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S, C252A. No alpha factor secretion signal in …PromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoTagRFP657-SpHis5
Plasmid#44846PurposeYeast tagging vector to create gene fusion with yeast optimized TagRF657 with SpHis5 selectionDepositorInsertyoTagRFP657
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRG645_lexO-AscI_LexA-TF_LEU2MX
Plasmid#154813PurposeInducible expression of AscI endonuclease in S. cerevisiaeDepositorInsertsAscI endonuclease
LexA-ER-B112
UseTagsFLAG and NLSExpressionYeastMutationPromoterACT1 and lexO_4-CYC1_coreAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRS305-mAID-LaM2
Plasmid#198413PurposeExpress mAID-Nb(LaM2) under the control of ADH1 promoter in budding yeastDepositorInsertmAID-Nb (LaM2)
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZJOM22
Plasmid#133629PurposeIntegration of EMC1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-EMC1-2GFP (EMC1 Budding Yeast)
UseTagsEGFPExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-TRP1
Plasmid#11007DepositorInsertTRP1 (TRP1 Budding Yeast)
UseTagsV5 and his6ExpressionBacterial and YeastMutationORF-TRP1 without stop codonPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mTID-HA3-KanMX6
Plasmid#227982PurposeTo insert biotin ligase, miniTurboID, at the C-terminus of genes; yeast BioID, KanMX6 markerDepositorInsertminiTurboID
UseTagsHA tagExpressionYeastMutationPromoterAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
p426 103Q GPD
Plasmid#1184DepositorInserthtt 103Q (HTT Human)
UseTagsGFPExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pRS314-URA3
Plasmid#11004DepositorInsertURA3 (URA3 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationWild typePromoterAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pEZY202
Plasmid#18705PurposepEG202 converted Gateway Destination vector for yeast two-hybrid bait vectorDepositorTypeEmpty backboneUseTwo-hybrid bait vectorTagsExpressionYeastMutationPromoterAvailable sinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAV0785
Plasmid#133507PurposeExpression of mCherry tagged PCNA to monitor DNA replicationDepositorInsertpUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mYFP-kanMX6
Plasmid#105147PurposeYeast gene targetingDepositorInsertmYFP-kanMX6
UseTagsmYFPExpressionBacterial and YeastMutationA206KPromoterAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG426-GAL-Alpha-synuclein-A53T
Plasmid#181722PurposeGalactose inducible expression of Alpha-synuclein-A53TDepositorInsertAlpha-synuclein (SNCA Human)
UseTagsExpressionYeastMutationA53TPromoterAvailable sinceJuly 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fCLIP-3xHA
Plasmid#188936PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fCLIP-3xHA
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-3XHA-fCLIP-NAT
Plasmid#188921PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fCLIP-NAT
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMAL728
Plasmid#169152PurposeEncodes OptoQ-AMP6DepositorInsertVP16-EL222
UseTagsSV40 NLSExpressionYeastMutationA79QPromoterAvailable sinceOct. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK
Plasmid#183943PurposeExpresses HA-tagged E. coli PPK from yeast GPD promoterDepositorInsertPPK (ppk E. coli)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK80 pHomL-RPL18Bp-Scarlet(rfp)-Link-VAM3-SSA1t-HomR (AmpR)
Plasmid#179064PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-VAM3, a marker for the vacuoleDepositorInsertRPL18Bp-Scarlet(rfp)-Link-VAM3
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pHLM
Plasmid#64168Purposeharbours marker genes HIS3, LEU2 and MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM319
Plasmid#84201PurposeDisturb the expression of miR319 in riceDepositorInsertmiR319
UseTagsExpressionBacterial, Plant, and Yeast…MutationPromoter2x35SAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only