8,173 results
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Plasmid#67553PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeExpressionYeastAvailable SinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only
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pSP561
Plasmid#139497PurposeExpression vector for the MS2-GFP protein in S. cerevisiaeDepositorInsertpADH1 MS2-GFPenvy tCYC1
ExpressionYeastMutation∆FGPromoterpADH1Available SinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastPromoteryeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIMVAu1
Plasmid#98301PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.DepositorInsertHMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
ExpressionYeastAvailable SinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-hpSC_URA3
Plasmid#22317DepositorInsertURA hairpin
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDR-GW AT1.03R122K/R126K
Plasmid#28005DepositorInsertAT1.03R122K/R126K
TagsmVenus and mseCFPExpressionYeastMutationR122K, R126KAvailable SinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDIV537
Plasmid#177704PurposePlasmid expressing optimized Cas9 and NAT marker and sgRNA targeting KU70 homolog in D. hanseniiDepositorInsertsCas9
Nat
tRNA-sgRNA-tRNA
UseCRISPRTagsSV40ExpressionBacterial and YeastPromoterRNR2p (Debaryomyces hansenii ), TDH3p (Candida lu…Available SinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEK22
Plasmid#210588PurposeReporter gene expression in B. dendrobatidisDepositorInsertBdH3pro-Hshph-G4S-nanoluc-ScADH1ter
UseLuciferaseTagsNanoLuc luciferaseExpressionBacterial and YeastPromoterBdH3proAvailable SinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNXRVb-HACre
Plasmid#78401Purposeconstitutive Cre expression vectorDepositorInserts3xHA-nls-Cre
hygMX6
ExpressionYeastAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKT127-SEP
Plasmid#41899DepositorTypeEmpty backboneTagssuperecliptic GFP (SEP)ExpressionYeastAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLexA
Plasmid#11342DepositorTypeEmpty backboneTagsLexA DNA Binding DomainExpressionYeastAvailable SinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
M4786 KanMX::hisG-URA3-HisG Disruptor Converter
Plasmid#51690PurposeYeast marker swap plasmid for converting KanMX3 to hisG-URA3-hisGDepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-GT
Plasmid#51231Purposebi-directional expression vector for yeast, galactose inducible, TRP1 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGAL1,10Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAV103
Plasmid#63193PurposepRS403 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionWorm and YeastAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX233
Plasmid#163762PurposeEmpty vector for Gal-inducible yeast expressionDepositorTypeEmpty backboneTagsHemagglutinin (HA) tagExpressionYeastPromoterGAL1Available SinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE6c
Plasmid#17466Purposemodified pENTR vector for C-terminal EYFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsEYFPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
BB3nK_ext_AD
Plasmid#108687PurposePlasmid for assembly of donor DNA templates consisting of three expression unitsDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT957
Plasmid#228293PurposeConstitutive mutant PhlTA expression with strong promoterDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterScPGK1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1035
Plasmid#228300PurposeConstitutive expression of LuxTA mutant with strong promoterDepositorInsertluxTA mutant
UseSynthetic BiologyTagsNuclear localization signal-Three copies of VP16 …ExpressionYeastMutationS116Y, W201RPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB2289
Plasmid#113396PurposeGalactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiaeDepositorInsertRed fluorescent protein, codon optimized for S. cerevisiae
ExpressionYeastAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pIM691
Plasmid#74641Purposealias pFA6a-ymTagBFP-hphNT1 - vector for C-terminal tagging of yeast genes with yeast-adapted mTagBFP and hygromycin selection markerDepositorInsertymTagBFP
ExpressionYeastPromoternoneAvailable SinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMVS142_pACT1_mCherry_Zif268_EBD_MCS_KAN
Plasmid#99049PurposeTranscription Factor chassis for activation domains. mCherry, Zif268 DNA binding domain, estrogen response domainDepositorInsertsExpressionYeastAvailable SinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP791_insul-(lexA-box)1-PminCYC1-Citrine-TCYC1
Plasmid#58432PurposeInduces Citrine expression from a promoter containing 1 lexA box in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)1-PminCYC1Available SinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB769
Plasmid#60138PurposeRed click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBR
ExpressionYeastPromoterMET17prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBBK43 pHomL-TDH3p-GFPdropout-SSA1t-HomR (AmpR)
Plasmid#179027PurposeParent vector for markerless yeast integration cassettes with untagged genesDepositorTypeEmpty backboneExpressionYeastAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)
Plasmid#178988PurposeSp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistanceDepositorInsertS. pyogenes Cas9
UseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceMay 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDS190
Plasmid#34977DepositorInsertePDZb
ExpressionYeastPromoterADHAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(414)/GFP-AUT7(414)
Plasmid#49424PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS414 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterATG8Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pXP118
Plasmid#26825DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBEVY-T
Plasmid#51232Purposebi-directional expression vector for yeast, constitutively active, TRP1 selectionDepositorTypeEmpty backboneExpressionYeastPromoterGPD/ADH1Available SinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJW1664
Plasmid#112038PurposeCEN/ARS plasmid expression of mitochondrial Su9-TagBFP marked with HISDepositorInsertSu9
TagsTagBFPExpressionYeastMutationamino acids 1-76Available SinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only