7,933 results

1. pAG416-GAL-FUS

   Plasmid

   #181711

   Purpose

   Galactose inducible expression of FUS

   Depositor

   Alejandro Chavez

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ZJOM86

   Plasmid

   #133634

   Purpose

   Integration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ANP1-GFP (ANP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEasyCas9

   Plasmid

   #184909

   Purpose

   Expresses the Streptococcus pyogenes Cas9 protein

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pYX113-mtGFP

   Plasmid

   #45049

   Depositor

   Benedikt Westermann

   Article

   Westermann et al Yeast. 2000 Nov;16(15):1421-7.

   Insert

   mtGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   May 17, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pRS315-ADH700p-yeCherry-p150-yeGFP-CYCt

   Plasmid

   #194519

   Purpose

   Dual fluorescence reporter for studying protein degradation

   Depositor

   Tommer Ravid

   Article

   Mashahreh et al Nat Commun. 2022 Dec 8;13(1):7588. doi:

   Insert

   TIF4631 leader sequence
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCTcon2-5INS_EV3

   Plasmid

   #102293

   Purpose

   Yeast Surface Display of 5Ins_16_EV3

   Depositor

   Sarel Fleishman

   Article

   Baran et al Proc Natl Acad Sci U S A. 2017 Oct 10;11

   Insert

   Evolved Insulin binding scFV
   

   Use

   Tags

   3x(G4S) Linker, G3S Linker, HA, and Myc

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDRF1 mStayGold-T2A-mCherry

   Plasmid

   #219834

   Purpose

   equimolar expression of mStaygold and mCherry in budding yeast

   Depositor

   Bas Teusink

   Insert

   mStayGold-T2A-mCherry
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pWS926

   Plasmid

   #123052

   Purpose

   LexA bacterial repressor

   Depositor

   Tom Ellis

   Article

   Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

   Insert

   LexA-NLS [3a]
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pJZC603

    Plasmid

    #62317

    Purpose

    sgRNA with 2x PP7 for yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Insert

    sgRNA + 2x PP7 RNA binding module
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIp128-P30-His-POL30

    Plasmid

    #99542

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA); LEU2 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    POL30

    Available since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. p413TEF1-ATR1(pYL573)

    Plasmid

    #178288

    Purpose

    Expresses NADPH-cytochrome P450 reductase 1 from A. thaliana (ATR1) in Saccharomyces cerevisiae. Centromeric HIS, PTEF1-ATR1-TCYC1

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    ATR1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPMVAu5 (R)

    Plasmid

    #98296

    Purpose

    Enhancing the upper-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.

    Depositor

    Claudia Vickers

    Article

    Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

    Insert

    PSSA1-ERG10-TNAT1-PRPL4A-ERG13-TEFM1-PRPL15A-tHMG1-TEBS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAG425GPD-ccdB-TAP

    Plasmid

    #14274

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUC-TALO8

    Plasmid

    #83409

    Purpose

    TRP1-ARS1-8xLacO (TALO8) minichromosome system for histone purification in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    TRP-ARS-8xLacO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Please see depositor comments below

    Promoter

    TRP1

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWS3868

    Plasmid

    #185745

    Purpose

    Sub-array backbone 4/4

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGWKS7

    Plasmid

    #139414

    Purpose

    Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

    Depositor

    James Fraser

    Article

    Spencer et al Fungal Genet Biol. 2020 May;138:103365.

    Insert

    mRuby3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJZC638

    Plasmid

    #62283

    Purpose

    Expresses MCP-VP64 and dCas9 in Yeast cells

    Depositor

    Wendell Lim, Stanley Qi

    Article

    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

    Inserts

    MCP-VP64
    dCas9
    

    Use

    Tags

    VP64

    Expression

    Yeast

    Mutation

    Nuclease activity has been deactivated by mutatio…

    Promoter

    pAdh and pGal10

    Available since

    April 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p426*-SpSgH

    Plasmid

    #136993

    Purpose

    Helper plasmid for SpCas9 gRNA cloning and expression

    Depositor

    Huimin Zhao

    Article

    Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDM291-tetR'-tup11Δ70

    Plasmid

    #41028

    Depositor

    Michael Nick Boddy

    Article

    Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

    Insert

    tetR'-tup11Δ70
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    tup11 starts at aa70

    Promoter

    Available since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pMJ240: pRS426-His-MBP-MmeFz2_Native

    Plasmid

    #205255

    Purpose

    S. cerevisiae expression vector for MmeFz2 native guide

    Depositor

    Feng Zhang

    Article

    Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

    Insert

    MmeFz2
    

    Use

    Tags

    10xHis, MBP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pI1-MTU-DO-URA

    Plasmid

    #160009

    Purpose

    Integrative vector for K. marxianus, targeting the LAC4 locus. Uracil selection.

    Depositor

    John Morrissey

    Article

    Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

    Type

    Empty backbone

    Use

    Kluyveromyces marxianus

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    n/a

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVG47

    Plasmid

    #164705

    Purpose

    yEVenus under the control of tetOpr

    Depositor

    Sahand Jamal Rahi

    Article

    Gligorovski et al Nat Commun. 2023 Jun 27;14(1):3810. doi:

    Insert

    tetOpr - yEVenus - CLN2 PEST - ADH1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFA6a-link-yoTagRFP657-Kan

    Plasmid

    #44955

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized TagRFp657 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoTagRFP657
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Htb2-GFP

    Plasmid

    #117669

    Purpose

    Colorize the nuclei

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    Htb2-GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains the gene encoding the Histone Htb2 from …

    Promoter

    pGAP

    Available since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYC56

    Plasmid

    #63910

    Purpose

    Recipient integrative vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ZJOM18

    Plasmid

    #133631

    Purpose

    Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-NAB2-GFP (NAB2 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pKmK.C2

    Plasmid

    #125060

    Purpose

    Storage plasmid for K.marxianus centromere

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    KmARS/CEN5
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pESC-URA-GFP-Ubc9wt

    Plasmid

    #20368

    Depositor

    Judith Frydman

    Article

    Kaganovich et al Nature. 2008 Aug 28. 454(7208):1088-95.

    Insert

    SUMO-conjugating enzyme Ubc9 (UBC9 Budding Yeast)
    

    Use

    Yeast/bacteria shuttle

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 28, 2009

    Availability

    Academic Institutions and Nonprofits only

31. pJO629

    Plasmid

    #104342

    Purpose

    Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.

    Depositor

    Alexander Varshavsky

    Article

    Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-2

    Insert

    pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1 (YAP5 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS406-CMV-lacI-3FLAG

    Plasmid

    #83410

    Purpose

    Expression of lacI in yeast

    Depositor

    Toshi Tsukiyama

    Article

    Unnikrishnan et al Nat Struct Mol Biol. 2010 Apr;17(4):430-

    Insert

    lacI repressor
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pXP416

    Plasmid

    #26842

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2010 Sep 10. ():.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMEL16

    Plasmid

    #107922

    Purpose

    HIS3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p413TEF-PKM2

    Plasmid

    #34608

    Depositor

    Markus Ralser

    Article

    Bluemlein et al Oncotarget. 2011 May;2(5):393-400.

    Insert

    PKM2 (PKM Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAct::Raf

    Plasmid

    #11341

    Depositor

    Michael Wigler

    Article

    Van Aelst et al Proc Natl Acad Sci U S A. 1993 Jul 1. 90

    Insert

    byr-2 (byr2 Fission Yeast)
    

    Use

    Tags

    Gal4 activation domain

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pDRF1 Cyterm-ytd8oxStayGold

    Plasmid

    #219853

    Purpose

    expression of Cyterm-ytd8oxStayGold in budding yeast

    Depositor

    Bas Teusink

    Insert

    Cyterm-ytd8oxStayGold
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pAG306-GPD-VMA1 chr I

    Plasmid

    #41896

    Purpose

    Yeast expression of VMA1.

    Depositor

    Dan Gottschling

    Article

    Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

    Insert

    VMA1 (VMA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains silent mutation at 342A-G and L87S mutat…

    Promoter

    GPD

    Available since

    April 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. ZJOM11

    Plasmid

    #133637

    Purpose

    Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pAGE2.0-i

    Plasmid

    #194639

    Purpose

    Empty pAGE2.0 plasmid with pBAD inducible promoter

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Type

    Empty backbone

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge