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8,173 results

Showing: 1601 - 1650 of 8173 results

  2. pGAPZ-STE2-1D4

    Plasmid
    #133423
    Purpose
    Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.
    Depositor
    Gerhard Wagner
    Insert
    STE2 (STE2 Budding Yeast)
    Expression
    Yeast
    Mutation
    C59S, C252A. No alpha factor secretion signal in …
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133423-plasmid-map-sequence-id-267651

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  12. pJRoC30_Th3

    Plasmid
    #188064
    Purpose
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173
    Insert
    PROSS-designed high-redox potential laccase from Trametes hirsuta
    Tags
    S. cerevisiae evolved α-factor prepro-leader sign…
    Expression
    Yeast
    Mutation
    20 mutations, described in publication
    Available Since
    Oct. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    188064-plasmid-map-sequence-id-370132

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  18. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available Since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

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  21. pUG-amdSYM-mak10TR

    Plasmid
    #75433
    Purpose
    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae
    Depositor
    John McCusker
    Inserts
    MAK10TR
    ApR
    amdSYM
    Expression
    Yeast
    Mutation
    N-terminal 740 bp of MAK10 ORF
    Promoter
    Native promoter and PTEF
    Available Since
    Jan. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    75433-plasmid-map-sequence-id-142819

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  22. THI VP64 pALD4 T-

    Plasmid
    #161481
    Purpose
    Non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_only_SV40NLS_pALD4_THi11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2-VP64
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161481-plasmid-map-sequence-id-324351

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  27. pACT2-μ3A

    Plasmid
    #198174
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3A
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    silent substitution in codon 225
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198174-plasmid-map-sequence-id-387537

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  28. pACT2-μ3B

    Plasmid
    #198175
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:
    Insert
    AP-3 μ3B
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Promoter
    ADH1
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198175-plasmid-map-sequence-id-387536

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  30. pMM0638

    Plasmid
    #129005
    Purpose
    Integration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2
    Depositor
    Megan McClean
    Article
    An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1
    Insert
    5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
    Expression
    Yeast
    Available Since
    Oct. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    129005-plasmid-map-sequence-id-261246

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  34. pAH237

    Plasmid
    #121146
    Purpose
    Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast
    Depositor
    Katsunori Tanaka
    Article
    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.
    Inserts
    gRNA cassette
    humanized Streptococcus pyogenes Cas9
    Use
    CRISPR
    Tags
    Flag
    Expression
    Yeast
    Promoter
    S.pombe rrk1 and nmt41
    Available Since
    Feb. 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    121146-plasmid-map-sequence-id-234796

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  36. pE4c

    Plasmid
    #17464
    Purpose
    modified pENTR vector for C-terminal GST tag fusion with your gene of interest
    Depositor
    Giovanna Benvenuto
    Article
    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.
    Type
    Empty backbone
    Use
    Modified gateway entry vector
    Tags
    GST
    Expression
    Bacterial, Insect, Mammalia…
    Available Since
    March 21, 2008
    Availability
    Academic Institutions and Nonprofits only
    17464-plasmid-map-sequence-id-8632

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  38. pGBW-m4046663

    Plasmid
    #153857
    Purpose
    Yeast expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    Expression
    Yeast
    Mutation
    wt
    Promoter
    pGAL1
    Available Since
    June 12, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153857-plasmid-map-sequence-id-300317

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  41. ZJOM11

    Plasmid
    #133637
    Purpose
    Integration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pCUP1-GFP-Tlg1 (TLG1 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133637-plasmid-map-sequence-id-265887

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  44. ZJOM1

    Plasmid
    #133632
    Purpose
    Integration of GFP-SED5 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis). Marker for yeast early golgi.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-GFP-SED5 (SED5 Budding Yeast)
    Tags
    EGFP
    Expression
    Yeast
    Available Since
    April 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    133632-plasmid-map-sequence-id-292100

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Showing: 1601 - 1650 of 8173 results