8,265 results
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Plasmid#185879PurposeA plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)DepositorTypeEmpty backboneExpressionYeastMutationNoneAvailable SinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only
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pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR
Plasmid#24549DepositorInsertpADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
ExpressionYeastMutation1. Truncated version of ADH700 promoter (SbfI/Spe…Available SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SPT6
Plasmid#10993DepositorAvailable SinceDec. 29, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yETSapphire-CaUra3
Plasmid#44650DepositorInsertTurbo Sapphire
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHS12-mCherry
Plasmid#25444DepositorInsertmCherry
TagsCOX4 PresequenceExpressionYeastAvailable SinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDB4281
Plasmid#98700PurposeA Cas9-encoding plasmid containing the 5' portion of the bsdMX marker and the rrk1 promoter/leader. When linearized by NotI, it serves as the gapped vector in the split-bsdMX systemDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDB4283
Plasmid#98702PurposeA plasmid containing the 3' portion of the bsdMX marker and the gRNA elements downstream of the target sequence. It serves as the PCR template for generating the gRNA insert in the split-bsdMX systemDepositorInsertgRNA PCR template
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECFP-Hygro
Plasmid#44647DepositorInsertyEmCFP
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG306GPD-ccdB-DsRed
Plasmid#14356DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS405-LEU2-TEF-KpolDcr1v2
Plasmid#85193PurposeIntegrating plasmid, K. polysporus Dcr1 under TEF promoterDepositorInsertDcr1
UseIntegrating, shuttle vectorExpressionYeastPromoterTEFAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNB769
Plasmid#60138PurposeRed click beetle luciferase from Promega driven by MET17 promoter.DepositorInsertCBR
ExpressionYeastPromoterMET17prAvailable SinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
Gal1/10p CAS9 Gal1/10p I-SceI
Plasmid#182519PurposeGalactose-Induced Expression vector for Cas9 and I-SceI.DepositorInsertsI-SceI
Cas9
ExpressionYeastAvailable SinceJan. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-Z3-Nat
Plasmid#232100PurposeYeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.DepositorTypeEmpty backboneUseCRISPRExpressionYeastAvailable SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Hyg
Plasmid#232095PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWS625
Plasmid#123046PurposeAdenosine GPCRDepositorInsertA2BR [3]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB2176
Plasmid#113395PurposeTy3-IN-GFP; alpha factor inducibleDepositorInsertTy3 with integrase-green fluorescent protein
TagsIN-GFPExpressionYeastAvailable SinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-EGFP-ccdB
Plasmid#14322DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoLSS-mKate2-Kan
Plasmid#44943PurposeYeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selectionDepositorInsertyoLSS-mKate2
ExpressionYeastAvailable SinceOct. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMT1420
Plasmid#228316PurposeHomoserine lactone (HSL)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoterHomoserine lactone (HSL)-regulatable synthetic pr…Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT950
Plasmid#228290Purpose2,4-Diacetylphloroglucinol (DAPG)-regulatable EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyExpressionYeastPromoter2,4-Diacetylphloroglucinol-regulatable synthetic …Available SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT957
Plasmid#228293PurposeConstitutive mutant PhlTA expression with strong promoterDepositorInsertphlTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)-Nuclear localization …ExpressionYeastMutationE41GPromoterScPGK1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1035
Plasmid#228300PurposeConstitutive expression of LuxTA mutant with strong promoterDepositorInsertluxTA mutant
UseSynthetic BiologyTagsNuclear localization signal-Three copies of VP16 …ExpressionYeastMutationS116Y, W201RPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P41nmt1-3FLAG
Plasmid#19337DepositorInsert3FLAG
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
p404-Grx5-GFP1-10
Plasmid#91952PurposeExpresses GFP1-10 in the mitochondrial matrixDepositorInsertGrx5-GFP1-10
ExpressionYeastPromoterGAPAvailable SinceJune 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG306-GPD-VMA1 chr I
Plasmid#41896PurposeYeast expression of VMA1.DepositorInsertVMA1 (VMA1 Budding Yeast)
ExpressionYeastMutationContains silent mutation at 342A-G and L87S mutat…PromoterGPDAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
ZJOM86
Plasmid#133634PurposeIntegration of ANP1-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast early golgi.DepositorAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pI1-MTU-DO-G418
Plasmid#160012PurposeIntegrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.DepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
M4755 KanMX::LEU2 Disruptor Converter
Plasmid#51685PurposeYeast marker swap plasmid for converting KanMX3 to LEU2DepositorAvailable SinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TKC-E2-Crimson-HDEL
Plasmid#38771DepositorInsertE2-Crimson
TagsLinker peptide and yeast ER retention signal sequ…ExpressionYeastPromoterTPI1Available SinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
LT2_14_GlyOx
Plasmid#177711PurposePlasmid for genome integration in X2 site expressing GlyRNA and OxPro (biosensors for glycolytic flux and oxidative stress)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
pTRX2_5xUAS-ymYPET-tCYC1
UseUsed as donor dna in genome integration after lin…ExpressionYeastAvailable SinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAG423GAL-ccdB-EGFP
Plasmid#14197DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAWH-largeT
Plasmid#133895PurposePositive control when used in combination with pBWH-p53. Negative control when used in combination with pBWH-Lam. Expression of Gal4AD-largeT hybrid protein. Homology regions for recombination with pBDepositorInsertLargeT
ExpressionYeastPromoterT7Available SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK
Plasmid#64188Purposeharbours marker gene LYS2DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
Designed VEGF-binding scFv G6des13
Plasmid#110213PurposeYeast Surface Display of G6des13DepositorInsertG6des13
Tagscmyc tagExpressionYeastMutationL A43P, L Q89L, L T94D, L P95T, H Y59H, H Y95FAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
VEGF binding scFv G6
Plasmid#111716PurposeYeast Surface Display of G6 as a reference starting point for designDepositorInsertG6
Tagscmyc tagExpressionYeastAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB3052(gRNA X-4, XI-3, XII-5)
Plasmid#73294PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-4, XI-3, and XII-5DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
ZJOM157
Plasmid#133640PurposeIntegration of GFP-PEP12 as an additional copy in genome, use auxotrophic marker URA3(Kluyveromyces lactis).Marker for yeast late endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
BRR2 pRS313
Plasmid#111411PurposeBrr2 (endogenous promoter)DepositorInsertBRR2
ExpressionYeastAvailable SinceOct. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-SKII-URA-GAL1-fSNAP-3xHA
Plasmid#188935PurposeIntegration cassette to create N-terminal fusions for fluorescent taggingDepositorInsertURA-GAL1-fSNAP-3xHA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-P81nmt1-3FLAG
Plasmid#19338DepositorInsert3FLAG
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHES836
Plasmid#89195PurposeExpresses mKate2 under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-mKate2
ExpressionYeastPromoterpZAvailable SinceJan. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
M3925 trp1::KanMX3 Disruptor Converter
Plasmid#51681PurposeYeast marker swap plasmid for converting Trp1 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYEXNH3
Plasmid#49024PurposeFX cloning Saccharomyces cerevisiae expression vector with GAL1 promoter and N-terminal 10x His tag and 3C protease cleavage siteDepositorTypeEmpty backboneTags3C protease site (PreScission site) and decaHis (…ExpressionYeastPromoterGAL1Available SinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCfB6630
Plasmid#106160PurposeEasyCloneYALI system-based yeast gRNA expression vector carrying a nourseothricin-resistance marker, helps to integrate vector pCfB6371 into Yarrowia lipolytica chromosomal location IntC_3, amp resistanceDepositorInsertunknown
ExpressionYeastAvailable SinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE4c
Plasmid#17464Purposemodified pENTR vector for C-terminal GST tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsGSTExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only