8,173 results
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Plasmid#216438PurposeConstitutive promoter for gene expression in S. pombe. Part type 2 following the YeastToolkit MoClo grammar.DepositorInsertPromoter eno101
UseSynthetic BiologyExpressionYeastMutationRemoval of any BsaI // BsmBI restriction siteAvailable SinceMarch 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymScarletI
Plasmid#193960PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymScarletI in budding yeastDepositorInsertymScarletI
ExpressionYeastPromoterTEF1Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIMVAd40R
Plasmid#98309PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;DepositorInsertERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCUPI NPD-GFP
Plasmid#1191DepositorInsertN-terminus of Sup35 (SUP35 Budding Yeast)
UseCup1 promoterTagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-ymYPET-CaURA3
Plasmid#118460PurposeYeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection. *An updated version of this plasmid is available: #168056 pFA6a-link-ymYPET-URA3.DepositorInsertymYPET
ExpressionYeastMutationYPET A206K, F208S, E232L, N235DAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MAP72_MS2
Plasmid#220623PurposeCassette vector to enable overexpression of rNTases as MS2 coat protein fusions in S. cerevisiae.DepositorTypeEmpty backboneUseShuttle vectorExpressionYeastAvailable SinceJune 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
RPL25-GFP (94)
Plasmid#24037DepositorAvailable SinceMarch 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTIN
Plasmid#162885PurposeOverexpression vector for the fission yeast Schizosaccharomyces pombeDepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJW1666
Plasmid#112040PurposeCEN/ARS plasmid for GAL-inducible empty reporter V5-TEV-EGFP tagged expression marked with URADepositorInsertNone
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM156
Plasmid#133636PurposeIntegration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ccdB-DsRed
Plasmid#14364DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJAID5XY
Plasmid#165051PurposeIntegrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.DepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
ExpressionYeastMutationOsTIR1 is codon-optimised for expression in S. ce…Available SinceMarch 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pK3FS
Plasmid#85776PurposeN-ICE plasmid pK3FSDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pABY-c46
Plasmid#201774PurposeExpresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeastDepositorInsertNbALFA
Tags40aa Linker, mNeonGreenExpressionYeastPromoterTEF1Available SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS416 Gal M337V YFP
Plasmid#27449DepositorInsertTDP-43 (TARDBP Human)
TagsYFPExpressionYeastMutationMethionine 337 mutated to Valine (M337V)Available SinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-ACC1*
Plasmid#126740Purposeyeast plasmid overexpressing ACC1 from Komagataella phaffiiDepositorInsertacc1*
ExpressionYeastMutationT3452GPromoterGAPAvailable SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-Hook3
Plasmid#198525PurposeExpression of GAL4 DNA-binding domain (BD)-Hook3 fusion protein in yeast (yeast two-hybrid assays)DepositorInsertHook3 (HOOK3 Human)
TagsGAL4-DNA binding domain (BD) fragmentExpressionYeastPromoterADH1Available SinceApril 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2791
Plasmid#63654PurposepCfB2791 is a vector for multiple integrations at sites sharing homology with Ty4Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
p415GPD-3xHA-GW
Plasmid#63781Purposeyeast expression vector p415GPD modified such that it is Gateway converted and introduces a 3xHA tag at the N-terminus of the insertDepositorTypeEmpty backboneTags3xHAExpressionYeastPromoterGPDAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2B-57
Plasmid#177927PurposeExpresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)DepositorArticleInsertEGFP
ExpressionBacterial and YeastPromoterTET3 (contains three rtTA binding sites)Available SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCCL-ReDNAP (Y427A)
Plasmid#52346PurposeCEN6/ARS4 plasmid, REV1 promoter>Recoded TP-DNAP1 (Y427A)DepositorInsertRecoded TP-DNAP
ExpressionBacterial and YeastPromoterREV1Available SinceJune 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pOGS539_GLuc-ns
Plasmid#226719PurposePlasmid enabling yeast-mediated expression of Gaussia luciferase (GLuc)DepositorInsertGaussia Luciferase
TagsHA tagExpressionYeastPromoterpTEF1Available SinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pILGFP95
Plasmid#83550PurposeUsed to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.DepositorInsertPDA1 promoter-yEGFP
ExpressionYeastPromoterPDA1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB4279
Plasmid#98698PurposeA control plasmid containing the intact bsdMX markerDepositorInsertCas9
UseCRISPRExpressionYeastAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
M3927 ura3::KanMX3 Disruptor Converter
Plasmid#51682PurposeYeast marker swap plasmid for converting URA3 to KanMX3DepositorInsertKanMX3
UseYeast marker swapExpressionYeastAvailable SinceJune 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-ccdB-HA
Plasmid#14243DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNH605-CCAAT-YFP
Plasmid#172132PurposeFluorescent reporter for HAP complex activityDepositorInsert4xCCAAT-YFP
UseVector for genomic integration into the yeast gen…ExpressionYeastPromoterCYC1 core promotor with 4 tandem repeats of the H…Available SinceNov. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6-mTFP1-KanMX6
Plasmid#69851PurposeC-terminal tagging with mTFP1 using KanMX6 as a selection marker in S. cerevisiae.DepositorInsertmTFP1
ExpressionYeastAvailable SinceApril 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ccdB-HA
Plasmid#14250DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046647
Plasmid#153940PurposeYeast expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionYeastMutationwtPromoterpGAL1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBS-SKII-3XHA-fSNAP-URA
Plasmid#188917PurposeIntegration cassette to create C-terminal fusions for fluorescent taggingDepositorInsert3XHA-fSNAP-URA
ExpressionYeastAvailable SinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0751
Plasmid#133485PurposeExpression in fission yeast from promoter pnmt1 , integrated at ura4 locusDepositorInsertpUra4(AfeI)-p(nmt1)-sfGFP-terminator(ScCYC1)
ExpressionYeastAvailable SinceNov. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMEL10
Plasmid#107916PurposeURA3 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPS2035
Plasmid#1368DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pXNubWtgate
Plasmid#105080PurposeYeast mating-based Split Ubiquitin Assay, positive controlDepositorTypeEmpty backboneTagsNubIExpressionYeastAvailable SinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJW1663
Plasmid#112037PurposeGal-Estradiol transcription activation expression cassette marked with KanRDepositorInsertGal DNA binding-estradiol binding-MSN2 activator
ExpressionYeastPromoterADH1Available SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAN201
Plasmid#129204PurposeyTRAP sensor of [RNQ+], detects aggregation of Rnq1DepositorAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-P3nmt1-3FLAG
Plasmid#19345DepositorInsertnatMX6
ExpressionYeastAvailable SinceSept. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGWKS10
Plasmid#139417PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.DepositorInsertClover
ExpressionYeastPromoterTEF1Available SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-HphMx
Plasmid#122922PurposeTRP1-HphMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningExpressionYeastAvailable SinceJuly 4, 2019AvailabilityAcademic Institutions and Nonprofits only