8,017 results
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Plasmid#133437PurposeMutant yeast STE2 coding sequence in vector for inducible expression in the methanogenic yeast Pichia pastoris.DepositorInsertSTE2 (STE2 Budding Yeast)
UseTagsExpressionYeastMutationC59S/C252APromoterAvailable sinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
5538 pUG2-SSA1
Plasmid#1247DepositorInsertSsa1 (SSA1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pDN0606 (pDEST-DHFR F[3] N-term,HygR)
Plasmid#210488PurposepDEST vector to tag gene of interest with DHFR F[3] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB11 (pDEST-DHFR F[1,2] C-term, NatR)
Plasmid#210485PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0605 (pDEST-DHFR F[1,2] N-term, NatR)
Plasmid#210487PurposepDEST vector to tag gene of interest with DHFR F[1,2] on the N-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKB12 (pDEST-DHFR F[3] C-term, HygR)
Plasmid#210486PurposepDEST vector to tag gene of interest with DHFR F[3] on the C-terminus to perform DHFR-PCA in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceNov. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
prp8-G736E
Plasmid#108450Purposeprp8-G736E in pRS313 backbone for expression in yeastDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionBacterial and YeastMutationchanged G736 to EPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0478
Plasmid#133498PurposeExpression of sfGFP targeted to the nucleus with two NLS signalsDepositorInsertpHis5(StuI)-p(tdh1)-NLSSV40-sfGFP-NLSSV40-natMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-VPS8-mCherry2Bx6
Plasmid#105273PurposePlasmid for pop-in/pop-out replacement of Vps8 with Vps8-mCherry2Bx6.DepositorInsertVps8 portion (VPS8 Budding Yeast)
UseTagsmCherry2Bx6ExpressionYeastMutationPromoterAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCfB2399
Plasmid#67550PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked amdSYM marker, integration into S. cerevisiae XI-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJZC620
Plasmid#62282PurposeExpresses dCas9, MCP-VP64, and PCP-VP64 in Yeast cellsDepositorInsertsMCP-VP64
PCP-VP64
dCas9
UseTagsVP64ExpressionYeastMutationNuclease activity has been inactivated by mutatio…PromoterpAdh and pTdh3Available sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-OSBP PH domain
Plasmid#58840PurposeExpresses GFP-Tagged OSBP PH domain in yeastDepositorInsertOxysterol-binding Protein PH domain (OSBP Human)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB4666
Plasmid#106144PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_1, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHES822
Plasmid#89193PurposeExpresses YFP under control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-YFP
UseTagsExpressionYeastMutationPromoterpZAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p41Hyg 1-F GW
Plasmid#58547PurposeCEN vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pM
Plasmid#64179Purposeharbours marker gene MET17DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceAug. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pG23A
Plasmid#102884PurposePlasmid used for the C-terminal tagging of Cdc14 with mCherry and the auxin-inducible degron in yeast.DepositorInsertCdc14/mCherry-AID(71-114)-NatMX (CDC14 Budding Yeast)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
YIPlac204TC-NLS-DsRed-Express2
Plasmid#25443DepositorInsertNLS-DsRed-Express2
UseTagsNLSExpressionYeastMutationPromoterAvailable sinceJuly 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold
Plasmid#157997PurposeExpression of mGold in yeast cellsDepositorInsertmGold
UseTagsExpressionYeastMutationmGold is mVenus with L46F;T63S mutationsPromoterpTDH(GAP promoter)Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
p42Hyg 1-F GW
Plasmid#58551PurposeMulticopy vector with Hygromycin resistance marker for single Gateway insertionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPTK002-2-pGAP
Plasmid#84961PurposeType 2, pGAP, Glyceraldehyde-3-phosphate dehydrogenase promoterDepositorInsertGlyceraldehyde-3-phosphate dehydrogenase promoter
UseSynthetic BiologyTagsExpressionYeastMutationBsaI site removed (1805 c to g)PromoterNonAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZJOM103
Plasmid#133664PurposeIntegration of SEC7-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorInsertp1k-SEC7-mTagBFP2 (SEC7 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB648
Plasmid#60140PurposeNanoLuc luciferase with a nuclear localization signal (NLS) driven by MET17 promoter.DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1010
Plasmid#111423PurposegRNA plasmid for cleavage of Candida albicans ADE2 - integrates at RPS1 (Part of Duet system)DepositorInsertsgADE-NT2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
LHP1463 - INP52
Plasmid#32432DepositorInsertINP52 (INP52 Budding Yeast)
UseTagsExpressionYeastMutationPromoterLacAvailable sinceNov. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRS303-LacZ
Plasmid#1169DepositorInsertLACZ
UseTagsGAL1:10 promoterExpressionYeastMutationPromoterAvailable sinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAV115
Plasmid#63182PurposepRS415 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYX223-mtGFP
Plasmid#45051DepositorInsertmtGFP
UseTagsExpressionYeastMutationPromoterGALAvailable sinceMay 24, 2013AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-backbone
Plasmid#172517PurposeYeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertionDepositorInsertgRNA cassette
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-KAR2-msGFP2-HDEL
Plasmid#160468PurposeYeast integration vector for the gene replacement of S. cerevisiae KAR2 fused to msGFP2 with an ER retrieval signalDepositorInsertKAR2-msGFP2-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-ccdB-EYFP
Plasmid#14224DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoPSmOrange-Kan
Plasmid#44951PurposeYeast tagging vector to create gene fusion with yeast optimized photoconvertible mOrange with Kan selectionDepositorInsertyoPSmOrange
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGALSc104(WT)
Plasmid#1146DepositorInsertHSP104 (HSP104 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAG304GAL-ccdB-EGFP
Plasmid#14183DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM96
Plasmid#133663PurposeIntegration of SEC26-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast early golgi.DepositorInsertp1k-SEC26-mTagBFP2 (SEC26 Budding Yeast)
UseTagsmTagBFP2ExpressionYeastMutationPromoterAvailable sinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICXC3GH
Plasmid#49023PurposeFX cloning Pichia pastoris expression vector with AOX1 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tagDepositorTypeEmpty backboneUseTags3C protease site (PreScission site), GFP, and dec…ExpressionYeastMutationPromoterAOX1Available sinceJan. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-HA
Plasmid#14229DepositorTypeEmpty backboneUseGateway destinationTags3HAExpressionYeastMutationPromoterAvailable sinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUC19-URA3-3'SEC13-GFP
Plasmid#25445DepositorInsertSec13-Green Fluorescent Protein
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 24, 2010AvailabilityAcademic Institutions and Nonprofits only