8,265 results
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ABF2-2EGFP-ter-URA3
Plasmid#184768PurposeMarker for yeast mitochondria. Integration of 2xGFP tag at ABF2 C terminus. Uses auxotrophic marker URA3(Candida albicans).DepositorAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1Tt
Plasmid#44509DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET3
Plasmid#107436PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG303GAL-ccdB-EYFP
Plasmid#14205DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET17
Plasmid#107443PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS415-SOH1
Plasmid#8622DepositorAvailable SinceNov. 15, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSK247
Plasmid#131148Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreenDepositorTypeEmpty backboneExpressionYeastPromoterpSUP35, min pCYC1Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pR208IIM1
Plasmid#45352DepositorInsertUBR1 mutant
ExpressionYeastMutationP406S, DE317NKPromoterADHAvailable SinceJuly 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCfB8383
Plasmid#126917PurposePlasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of tHMG1DepositorInserttHMG1
ExpressionYeastAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
Osa-pCAMBIA-1301-STTM159
Plasmid#84195PurposeDisturb the expression of miR159 in riceDepositorInsertmiR159
ExpressionBacterial, Plant, and Yeast…Promoter2x35SAvailable SinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPT-PCR C2
Plasmid#141247PurposePCR cloned Phaeodactylum tricornutum Mitochondrial Genome - Clone 2DepositorInsertMitochondrial Genome of Phaeodactylum tricornutum
UseSynthetic Biology; Diatom expressionExpressionBacterial and YeastMutationRepeat region removed, additional minor mutations…Available SinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
GTH-y
Plasmid#81105PurposeGene Tagging vector HIS/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…Available SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET14
Plasmid#107441PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
KBB285
Plasmid#185076PurposeRFA3-GFP fusionDepositorInsertRFA3
TagsGFPExpressionYeastAvailable SinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB284
Plasmid#185075PurposeRFA2-GFP fusionDepositorInsertRFA2
TagsGFPExpressionYeastAvailable SinceJuly 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMJ127: pRS426-His-MBP-NlovFz2_PSP1
Plasmid#205257PurposeS. cerevisiae expression vector for NlovFz2 reprogrammed guide (targeting PSP1)DepositorInsertNlovFz2
Tags10xHis, MBPExpressionYeastAvailable SinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFP1F5
Plasmid#165064PurposeCharacterisation of TEF1 promoter using yEGFP as reporter gene and PGK1 terminatorDepositorInsertP-URA3>KlURA3>T-AgTEF1-P-TEF1> (BamHI)yEGFP>T-PGK1-T-URA3
ExpressionYeastMutationWTAvailable SinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG426GPD-ccdB-ECFP
Plasmid#14180DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG413GPD-ccdB-DsRed
Plasmid#14358DepositorTypeEmpty backboneUseGateway destinationTagsDsRedExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB3036(XI-1 MarkerFree)
Plasmid#73274PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-1 (Chr XI: 67491..68573)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLK89 - Edit-directing plasmid cloning insert
Plasmid#98846PurposeContains the insert used for step 2 of edit-directed plasmid cloningDepositorInsertKanR
UseCRISPRExpressionYeastMutationChanged sequence to remove a MluI cut siteAvailable SinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pRS315e_pGal-PmCDA1
Plasmid#79612PurposeExpresses PmCDA1 in yeast cellsDepositorInsertPmCDA1
ExpressionYeastPromoterpGal1Available SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJW1673
Plasmid#112047PurposeCEN/ARS plasmid for GAL-inducible SCT1-V5-TEV-EGFP expression marked with URADepositorInsertSCT1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-302
Plasmid#89974Purposeprp16-302 allele in TRP plasmidDepositorAvailable SinceAug. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
RB793
Plasmid#199121PurposeThis plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genomeDepositorInsert100 bp barcoding cassette
ExpressionYeastAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
RB794
Plasmid#199122PurposeThis plasmid contains a barcoding cassette which gets integrated at Neut5 locus in Candida albicans genomeDepositorInsert100 bp barcoding cassette
ExpressionYeastAvailable SinceAug. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS303 prp8-101
Plasmid#111307Purposeprp8-101DepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1TCbt
Plasmid#44514DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046207
Plasmid#145559PurposeYeast Expression plasmid for SARS-CoV-2 nsp6DepositorInsertSARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
TagsCleavable thrombin;6xHISExpressionYeastMutationSaccharomyces cerevisiae recode 1PromoterpGAL1Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKT0174
Plasmid#8720DepositorInsertyECFP
TagsCodon optimized linkerExpressionYeastMutationyEGFP with F64L, S65T, Y66W, N144I, M153T, V163AAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
p42Neo 3-F GW
Plasmid#58558PurposeMulticopy with G418 resistance marker for triple Gateway insertionDepositorTypeEmpty backboneExpressionYeastAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
ZJOM108
Plasmid#133665PurposeIntegration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.DepositorAvailable SinceApril 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB4132
Plasmid#106127PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked hygromycin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LHP416-PAN1-HA
Plasmid#32159DepositorAvailable SinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-msGFP2-STE13
Plasmid#177106PurposeExpresses msGFP2-Ste13 in yeast cellsDepositorInsertSTE13 (STE13 Budding Yeast)
ExpressionYeastAvailable SinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo*
Plasmid#115427PurposeExpresses a regulatable secretory cargo (lacking an ER export signal) for yeast at the TRP1 locusDepositorInsertpOst1-DsRed-Express2-FKBP(LV)(C22V)
ExpressionYeastPromoterTPI1Available SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAG306GAL-ccdB-EYFP
Plasmid#14211DepositorTypeEmpty backboneUseGateway destinationTagsEYFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-CHC1-msGFPx3
Plasmid#105256PurposePlasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.DepositorAvailable SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAV0479
Plasmid#133499PurposeExpression of sfGFP excluded from the nucleus with two NES signalsDepositorInsertpHis5(StuI)-p(tdh1)-NESwis1-sfGFP-NESmia1-natMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Destination Vector
Plasmid#125754PurposeDestination vector for assembling gRNA fragments in the second round of assembly.DepositorInsertpTDH3-GFPdropout-tTDH1
UseSynthetic BiologyExpressionYeastAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
Desmin/pAS2-1
Plasmid#97407PurposeDesmin (aa 30 – 103) in DNA-binding domain (BD) vector, used for yeast-two hybridDepositorInsertDesmin (DES Human)
ExpressionYeastAvailable SinceAug. 21, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCfB3041(gRNA X-3)
Plasmid#73283PurposeEasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3DepositorInsertguiding RNA
UseCRISPR; GrnaExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pITI1
Plasmid#166094PurposeGal inducible expression of dCas9-FokIDepositorInsertdCas9-FokI
UseCRISPRExpressionYeastMutationspCas9-D10A, H840APromoterGal-inducibleAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
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D5 gene in PETCON vector
Plasmid#87626Purposeexpresses D5 allosteric Zn2+ and fluorescein binding antibody for yeast displayDepositorInsertDesigned allosteric Zn2+ and fluorescein binding antibody scFv D5
Tags3x(G4S) Linker and cMyc tag on backboneExpressionYeastAvailable SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVG48
Plasmid#164706PurposertTA under the control of PGK1 promoterDepositorInsertPGK1pr - rtTA - CYC1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAV0543
Plasmid#133493PurposeExpression of nucleo-cytoplasmic mCherry in P-cellsDepositorInsertpLys3(BstZ17I)-p(map3)-mCherry-terminator(ScADH1)-natMX
ExpressionYeastAvailable SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only
