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Plasmid#125704PurposeYeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selectionDepositorInsertymNeonGreen
UseTagsymNeonGreenExpressionYeastMutationPromoterAvailable sinceJune 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRS306-OsTIR1F74A
Plasmid#198416PurposeExpresses OsTIR1F74A under the control of ADH1 promoter in budding yeastDepositorInsertOsTIR1F74A
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMay 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
UseTagsExpressionYeastMutationI777K, L900SPromoterAvailable sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-ccdB-EGFP
Plasmid#14203DepositorTypeEmpty backboneUseGateway destinationTagsEGFPExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRN1120
Plasmid#101750PurposeMulti-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEV-G2-Km ymNeongreen
Plasmid#193959PurposeTEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeastDepositorInsertymNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPM28 (eroGFP CEN/ARS URA3)
Plasmid#20131DepositorInserteroGFP (KAR2 Budding Yeast, jellyfish)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 11, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_Sap
Plasmid#206849PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was domesticated for SapI and integrated into the chloroplast fragment.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP2
Plasmid#206847PurposeA plasmid containing a 15,249 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP1
Plasmid#206846PurposeA plasmid containing a 14,519 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC5GGv1
Plasmid#194645PurposepSC5 Golden-Gate Cloning Plasmid (BsaI - Site 1)DepositorTypeEmpty backboneUseSynthetic Biology; Diatom expressionTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomTagBFP2-SpHis5
Plasmid#44839PurposeYeast tagging vector to create gene fusion with yeast optimized mTagBFP2 with SpHis5 selectionDepositorInsertyomTagBFP2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pE5n
Plasmid#17459Purposemodified pENTR vector for N-terminal ECFP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsECFPExpressionBacterial, Insect, Mammalia…MutationPromoterAvailable sinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomRuby2-SpHis5
Plasmid#44858PurposeYeast tagging vector to create gene fusion with yeast optimized mRuby2 with SpHis5 selectionDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 16, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-6xGLY-3xFLAG-HIS3MX6
Plasmid#20753DepositorTypeEmpty backboneUsePcr-based c-terminal taggingTagsExpressionYeastMutationPromoterAvailable sinceMarch 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSB819-PKR-hum
Plasmid#20030DepositorInsertProtein kinase R (EIF2AK2 Human)
UseTagsExpressionYeastMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
GFP-HDEL
Plasmid#129098PurposeFor labeling endoplasmic reticulum (ER) in S. cerevisiae with GFP-HDELDepositorInsertTEF1p-SS-3xGlyAla-GFP-HDEL
UseTagsExpressionYeastMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGBKCg
Plasmid#20162DepositorTypeEmpty backboneUseTagsGal4 DBDExpressionYeastMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAG414GPD-dCas9-VPR
Plasmid#63801PurposepAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vectorDepositorInsertdCas9-VPR
UseCRISPR and Synthetic BiologyTagsVPR (VP64-p65-Rta)ExpressionYeastMutationPromoterGPDAvailable sinceApril 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB
Plasmid#14145DepositorTypeEmpty backboneUseGateway destinationTagsExpressionYeastMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pNTI661 pRPR1(TetO)-sgRNA
Plasmid#139475PurposeVector for tet-regulated sgRNA expression in yeastDepositorInsertsingle guide RNA scaffold
UseCRISPRTagsExpressionYeastMutationPromoterpRPR1(TetO)Available sinceNov. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mBaojin-T2A-mCherry
Plasmid#219842Purposeequimolar expression of mBaojin and mCherry in budding yeastDepositorInsertmBaojin-T2A-mCherry
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAS1NB c Rosella I
Plasmid#71245Purposea biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Cytosolic localization.DepositorInsertRosella
UseTagsExpressionYeastMutationPromoterPGK1Available sinceNov. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pV1524
Plasmid#111431PurposeCaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)DepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p415-GalL-Cas9-CYC1t
Plasmid#43804PurposeA human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoterDepositorArticleInsertHuman Optimized S. pyogenes Cas9
UseCRISPRTagsExpressionYeastMutationPromoterGalLAvailable sinceMarch 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
gRNA-trp-HYB
Plasmid#64331Purposetrp1 deletion gRNA cassette carried by pRS42HDepositorInsertgBlock product of trp1 deletion gRNA cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSFS2A-CaKan
Plasmid#189565PurposeCodon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selectionDepositorInsertCaKan
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pROS17
Plasmid#107931PurposeTRP1 based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
LT2_12_GlyRNA
Plasmid#177710PurposePlasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)DepositorInsertspTEFmut8-mTurquoise2-RNA_2.6--tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
UseTagsExpressionYeastMutationPromoterTef1Available sinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
KBB418
Plasmid#185088PurposeSwi6L-GFP with nuclear export signal mutated to alaninesDepositorInsertSWI6
UseTagsGFPExpressionYeastMutationSwi6 nuclear export signal L to A mutationsPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
KBB460
Plasmid#185094PurposeNMA111-GFP wild type under endogenous promoterDepositorInsertNMA111
UseTagsGFPExpressionYeastMutationNMA111-GFPPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
LT2_7_OxPro
Plasmid#177709PurposePlasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)DepositorInsertspTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1
UseUsed as donor dna in genome integration after lin…TagsExpressionYeastMutationPromoterAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415 TEF roGFP2‐Tsa2ΔCPΔCR
Plasmid#83239PurposeThe catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.DepositorInsertroGFP2-Tsa2dCpdCr
UseTagsroGFP2 fusion with Tsa2dCpdCrExpressionYeastMutationPromoterTEFAvailable sinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ E.coli, Synthetic)
UseTagsExpressionYeastMutationPromoter5 XREs+ minimal Cytochrome CAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pG4
Plasmid#162605PurposeEdit Ade2 gene in yeastDepositorInsertTef1-Cas9 with RPL25 Intron
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-tdTomato-hphMX6
Plasmid#105157PurposeYeast gene targetingDepositorInserttdTomato-hphMX6
UseTagstdTomatoExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS306-URA3-PHIS3-PfV-GS-Sapphire
Plasmid#116932PurposeYeast integrative vector for the exression of the 40nm GEM under the HIS3 promoterDepositorInsertPfv
UseTagsSapphireExpressionYeastMutationPromoterHIS3Available sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mCherry-hphMX6
Plasmid#105156PurposeYeast gene targetingDepositorInsertmCherry-hphMX6
UseTagsmCherryExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only