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We narrowed to 475 results for: piggybac vectors

Showing: 1 - 40 of 475 results

  2. piggyBAC-U6-GFP-WPRE

    Plasmid
    #200030
    Purpose
    piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs
    Depositor
    Jay Shendure
    Type
    Empty backbone
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    U6, EF1a
    Available since
    May 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    200030-plasmid-map-sequence-id-393204

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  3. p101_CRISPRai_PiggyBac

    Plasmid
    #213777
    Purpose
    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.
    Depositor
    Howard Chang
    Article
    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103
    Inserts
    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    EF1a (EF-1-alpha intron a) and Tet_On_TRE
    Available since
    May 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    213777-plasmid-map-sequence-id-431902

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  9. pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

    Plasmid
    #211821
    Purpose
    PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment
    Depositor
    Erik Sontheimer
    Article
    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.
    Insert
    SpCas9-ABE8e, tracrRNA and Puromycin resistance gene
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    chicken β-actin promoter, U6 promoter and hPGK pr…
    Available since
    Jan. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    211821-plasmid-map-sequence-id-418440

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  12. Vector 1174D

    Plasmid
    #200012
    Purpose
    Fluorescent based sex separator in Aedes aegypti (SEPARATOR)
    Depositor
    Omar Akbari
    Article
    Weng et al bioRxiv. 2023 Jun 16:2023.06.16.545348.
    Insert
    Engineered dobulesex splicing module
    Use
    Synthetic Biology
    Tags
    Expression
    Insect
    Mutation
    Engineered dobulesex splicing module
    Promoter
    Hr5IE1
    Available since
    June 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    200012-plasmid-map-sequence-id-395199

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  13. Vector 1174K

    Plasmid
    #221017
    Purpose
    Fluorescent based sex separator in Anopheles species mosquitoes (SEPARATOR)
    Depositor
    Omar Akbari
    Insert
    Engineered dobulesex splicing module (Anopheles gambiae)
    Use
    Synthetic Biology
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    221017-plasmid-map-sequence-id-441587

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  14. pPB-CAG-Venus-Akaluc

    Plasmid
    #209768
    Purpose
    Venus-Akaluc is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    Venus-Akaluc
    Use
    Luciferase; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209768-plasmid-map-sequence-id-417214

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  17. pPB-CAG-NPHP1

    Plasmid
    #209769
    Purpose
    Human NPHP1 (Nephrocystin 1) gene is inserted into pPB-PGK-destination (Plasmid #60436).
    Depositor
    Yohei Hayashi
    Insert
    NPHP1 (NPHP1 Human, Synthetic)
    Use
    Piggybac transposon
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    209769-plasmid-map-sequence-id-417213

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  21. pPV-TetO-SpCas9-GR-iC-ERiH (CRONUS-Hygro)

    Plasmid
    #100597
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Hygro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100597-plasmid-map-sequence-id-207349

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  22. pPV-TetO-SpCas9-GR-iC-ERiP (CRONUS-Puro)

    Plasmid
    #100596
    Purpose
    piggyBac vector expressing Dual-regulated Cas9 (Puro resistance)
    Depositor
    Akitsu Hotta
    Article
    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1
    Inserts
    CRISPR Cas9 fused with human Glucocorticoid Receptor
    mCherry
    rtTA-M2
    Use
    Piggybac vector
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for human codon usage
    Promoter
    Dox-inducible TetO promoter and Human EEF1A1 prom…
    Available since
    April 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    100596-plasmid-map-sequence-id-207339

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  24. PB-TET

    Plasmid
    #20909
    Purpose
    piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)
    Depositor
    Andras Nagy
    Article
    Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi
    Insert
    Gateway Destination Vector
    Use
    piggybac
    Tags
    IRES-bGeo
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 5, 2009
    Availability
    Academic Institutions and Nonprofits only
    20909-plasmid-map-sequence-id-174009

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  25. PB-TET-PH-lox66FRT

    Plasmid
    #139561
    Purpose
    piggyBac expression vector with TET-ON system and puromycin selection
    Depositor
    Minoru Ko
    Insert
    PGK-puroR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139561-plasmid-map-sequence-id-274608

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  26. PB-TET-ZH-lox66FRT

    Plasmid
    #139563
    Purpose
    piggyBac expression vector with TET-ON system and Zeocin selection
    Depositor
    Minoru Ko
    Insert
    PGK-ZeoR-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139563-plasmid-map-sequence-id-274610

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  27. PB-TET-BH-lox66FRT

    Plasmid
    #139562
    Purpose
    piggyBac expression vector with TET-ON system and Blasticidin selection
    Depositor
    Minoru Ko
    Insert
    PGK-BSD-pA cassette, inserted in PB-TET vector
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139562-plasmid-map-sequence-id-274605

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  29. PB Neo* PB

    Plasmid
    #138413
    Purpose
    Minimal piggyBac ITR-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Piggybac
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138413-plasmid-map-sequence-id-316185

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  30. pScarlessHD-DsRed

    Plasmid
    #64703
    Purpose
    Vector for generating dsDNA donors for homology-directed repair. Contains the visible marker 3xP3-DsRed flanked by piggyBac termini.
    Depositor
    Kate O'Connor-Giles
    Insert
    piggyBac-3xP3-DsRed
    Use
    Tags
    Expression
    Mutation
    Promoter
    3xP3
    Available since
    Dec. 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    64703-plasmid-map-sequence-id-443894

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  31. PB-SAM

    Plasmid
    #102559
    Purpose
    Piggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.
    Depositor
    Ying Liu
    Article
    Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64
    Inserts
    MS2-P65-HSF1_T2A_Hygro
    dCAS9(D10A, N863A)-VP64_T2A_Blast
    Use
    CRISPR; Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9 and N55K in MS2
    Promoter
    CAG and EF1A
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    102559-plasmid-map-sequence-id-193885

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  32. PB-TA-ERP2

    Plasmid
    #80477
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80477-plasmid-map-sequence-id-179966

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  33. pDEST-PB-R4R3

    Plasmid
    #139518
    Purpose
    Provides two PiggyBac transposon sequences between gene cassettes in the destination vector
    Depositor
    Minoru Ko
    Type
    Empty backbone
    Use
    Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    139518-plasmid-map-sequence-id-274611

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  34. PB-TAC-ERN

    Plasmid
    #80475
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80475-plasmid-map-sequence-id-477521

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  35. PB-TAC-ERP2

    Plasmid
    #80478
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80478-plasmid-map-sequence-id-404034

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  36. PB-TA-ERN

    Plasmid
    #80474
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80474-plasmid-map-sequence-id-151580

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  37. PB-TAG-ERN

    Plasmid
    #80476
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and neomycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80476-plasmid-map-sequence-id-153664

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  38. PB-TAG-ERP2

    Plasmid
    #80479
    Purpose
    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible GFP and constitutive rtTA and puromycin resistance)
    Depositor
    Knut Woltjen
    Article
    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:
    Type
    Empty backbone
    Use
    Piggybac transposon
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    80479-plasmid-map-sequence-id-151592

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  39. PB-eDIO-EYFP

    Plasmid
    #104388
    Purpose
    PiggyBac plasmid encoding EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.
    Depositor
    Volker Busskamp
    Article
    Klapper et al Sci Rep. 2017 Oct 31;7(1):14450. doi: 10
    Insert
    Enhanced Yellow Fluorescent Protein
    Use
    Cre/Lox; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    104388-plasmid-map-sequence-id-202374

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Showing: 1 - 40 of 475 results