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Showing: 1 - 50 of 2321 results

  3. pCLucIPZ

    Plasmid
    #53222
    Purpose
    Constitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interest
    Depositor
    Thorsten Stiewe
    Article
    Charles et al Nat Commun. 2014 Jun 3;5:3981. doi: 10.1
    Inserts
    Cypridina luciferase
    non-silencing shRNA
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    53222-plasmid-map-sequence-id-342548

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  5. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  8. cycLuc_PPVs

    Plasmid
    #119208
    Purpose
    Circularly permutated firefly luciferase with PPVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with PPVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mutation
    Promoter
    CMV
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    119208-plasmid-map-sequence-id-244622

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  10. P9_TEVs_cLuc

    Plasmid
    #119303
    Purpose
    Expresses C-terminus of split luciferase connected to P9 coil via TEVs cleavable linker/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc connected to P9 coil via TEVs cleavable linker
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    119303-plasmid-map-sequence-id-231805

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  12. cycLuc_SuMMVs

    Plasmid
    #119210
    Purpose
    Circularly permutated firefly luciferase with SuMMVs cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with SuMMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119210-plasmid-map-sequence-id-232040

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  13. AP4mS_PPVs_P3_cLuc

    Plasmid
    #119300
    Purpose
    Expresses C-terminus of split luciferase fused to P3 coil, PPVs cleavable linker and autoinhibitory coil AP4/for split protease-cleavable orthogonal Coiled-coil (SPOC) logic
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    split cLuc fused to P3coil-PPV cleavage site and autoinhibitory coil AP4mS
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119300-plasmid-map-sequence-id-232041

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  14. cycLuc_SbMVs

    Plasmid
    #119209
    Purpose
    Circularly permutated firefly luciferase with SbMV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with SbMVp cleavage site
    Use
    Luciferase
    Tags
    AU1 tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    119209-plasmid-map-sequence-id-231830

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  19. mCherry-Occludin-N-10

    Plasmid
    #55112
    Purpose
    Localization: Tight Junctions, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55112-plasmid-map-sequence-id-257392

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  22. mEmerald-Occludin-N-10

    Plasmid
    #54212
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. mEmerald-Occludin-C-14

    Plasmid
    #54211
    Purpose
    Localization: Tight Junctions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54211-plasmid-map-sequence-id-140287

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  27. tdEos-Occludin-N-10

    Plasmid
    #57651
    Purpose
    Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Occludin (OCLN Human)
    Use
    Tags
    tdEos
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  28. pB-T-PAF-Clu

    Plasmid
    #215380
    Purpose
    High expression of Clusterin for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215380-plasmid-map-sequence-id-431850

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  37. pET-PesaR-Clux

    Plasmid
    #47658
    Purpose
    PesaR-C promoter, luxCDABE, and terminator in pET17b(AmpR)
    Depositor
    Cynthia Collins
    Article
    Shong et al ACS Synth Biol. 2013 Jul 23.
    Insert
    PesaR-C
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Additional esa box between -35 site and the origi…
    Promoter
    PesaR-C
    Available since
    Oct. 1, 2013
    Availability
    Academic Institutions and Nonprofits only

  39. pET28-his-3C_Clu3B6p

    Plasmid
    #100093
    Purpose
    expresses easily crystallizable engineered 14-3-3 sigma variant fused to the HSPB6 phosphorylatable peptide
    Depositor
    Nikolai Sluchanko
    Article
    Sluchanko et al Sci Rep. 2017 Sep 20;7(1):12014. doi: 10
    Insert
    14-3-3 sigma human (SFN Human)
    Use
    Tags
    HSPB6 peptide and His6
    Expression
    Bacterial
    Mutation
    75-77 residues changed to AAA to facilitate cryst…
    Promoter
    T7
    Available since
    Sept. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    100093-plasmid-map-sequence-id-193872

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  40. pHUE-Clu-H7-GFP

    Plasmid
    #232021
    Purpose
    Expression of a His6-Ubiquitin-Clu(241-262)-eGFP fusion protein
    Depositor
    Franz-Ulrich Hartl
    Insert
    Clu(241-262)-eGFP (CLU Human)
    Use
    Tags
    His6-Ubiquitin
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    232021-plasmid-map-sequence-id-470415

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  41. pB-T-PAF-Clu DD1

    Plasmid
    #215390
    Purpose
    High expression of the Clusterin(A108E,L109S,E112K,P115Q,K304A,E307H) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Ala108Glu, p.Leu109Ser, p.Glu112Lys, p.Pro115Gl…
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215390-plasmid-map-sequence-id-431790

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  42. pB-T-PAF-Clu DD2

    Plasmid
    #215391
    Purpose
    High expression of the Clusterin(Y125F,G132E,L135K,R138E,Q139A,E142S) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Tyr125Phe, p.Gly132Glu, p.Leu135Lys, p.Arg138Gl…
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215391-plasmid-map-sequence-id-431789

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  43. pB-T-PAF-Clu DD3

    Plasmid
    #215392
    Purpose
    High expression of the Clusterin(K123Q,R127Q,R130Q,R138Q,R282Q,R286Q,R289Q) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Lys123Gln, p. Arg127Gln, p.Arg130Gln, p.Arg138G…
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215392-plasmid-map-sequence-id-431818

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  44. pB-T-PAF-Clu AB1

    Plasmid
    #215393
    Purpose
    High expression of the Clusterin(V26D,M34W,Q37E) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Val26Asp, p.Met34Trp, p.Arg37Glu
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215393-plasmid-map-sequence-id-431821

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  45. pB-T-PAF-Clu AB2

    Plasmid
    #215394
    Purpose
    High expression of the Clusterin(V389K) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Val389Lys
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215394-plasmid-map-sequence-id-431812

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  46. pB-T-PAF-Clu TL1

    Plasmid
    #215381
    Purpose
    High expression of the Clusterin(p.Arg214_Leu238del) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Arg214_Leu238del
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215381-plasmid-map-sequence-id-431849

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  47. pB-T-PAF-Clu TL2

    Plasmid
    #215382
    Purpose
    High expression of the Clusterin(Y204S,Y206S,L207S,F209S,L211S,F218S,F219S,F220S) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Tyr204Ser, p.Tyr206Ser, p.Leu207Ser, p.Phe209Se…
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215382-plasmid-map-sequence-id-431816

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  48. pB-T-PAF-Clu TL3

    Plasmid
    #215383
    Purpose
    High expression of the Clusterin(L229S,M230S,F232S,Y235S) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Leu229Ser, p.Met230Ser, p.Phe232Ser, p.Tyr235Ser
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215383-plasmid-map-sequence-id-431808

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  49. pB-T-PAF-Clu TL2+3

    Plasmid
    #215384
    Purpose
    High expression of the Clusterin(Y204S,Y206S,L207S,F209S,L211S,F218S,F219S,F220S,L229S,M230S,F232S,Y235S) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Tyr204Ser, p.Tyr206Ser, p.Leu207Ser, p.Phe209Se…
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215384-plasmid-map-sequence-id-431801

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  50. pB-T-PAF-Clu TL4

    Plasmid
    #215385
    Purpose
    High expression of the Clusterin(R227Q) mutant for purification.
    Depositor
    Franz-Ulrich Hartl
    Insert
    CLU (CLU Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    p.Arg227Gln
    Promoter
    tetO
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    215385-plasmid-map-sequence-id-431804

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Showing: 1 - 50 of 2321 results