We narrowed to 17,933 results for: multi
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Plasmid#31938PurposeHuman OCT4 genomic targeting donorDepositorAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by
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ampkar
Plasmid#35097PurposeA fluorescent reporter of AMPK activity and cellular energy stressDepositorPublicationInsertampkar
TagsECFP and VenusExpressionBacterialMutationnaPromoterCMVAvailable sinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
piRFP720-N1
Plasmid#45461DepositorInsertiRFP720
ExpressionMammalianMutationT2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…PromoterCMVAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-EF1-Luc2-P2A-tdTomato
Plasmid#72486PurposeExpress Luc2 and tdTomato under EF1 promoterDepositorInsertLuc2-P2A-tdTomato
UseLentiviralExpressionMammalianPromoterEF1Available sinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCRCT
Plasmid#60621PurposePlasmid encoding iCas9, tracrRNA and crRNAsDepositorPublicationInsertsiCas9
tracrRNA
UseCRISPRTagsFLAG and SV40 NLSExpressionYeastMutationchanged Aspartate 147 to Tyrosine, Proline 411 to…PromoterRPR1p and TEF1pAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pML107
Plasmid#67639PurposeExpresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains LEU2 marker for yeast transformationDepositorInsertsCas9
single guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52 and pTDH3 (aka GAP promoter)Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX261-U6-DR-hEmx1-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro
Plasmid#42337PurposeDual expression plasmid of human codon-optimized SpCas9 and a gRNA to the human Emx1 locus, can be used to test SpCas9 cleavage in cell lines of choice.DepositorPublicationInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianAvailable sinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDD282
Plasmid#66823PurposeGFP^SEC^3xFlag vector with ccdB markers for cloning homology armsDepositorInsertGFP-C1^SEC^3xFlag
UseCRISPR and Cre/LoxTags3xFlag and C. elegans codon-optimized GFPExpressionWormAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pPlatTET-gRNA2
Plasmid#82559PurposeAll in one vector which contains Cas9 peptide array (linker length: 22aa), antibody-sfGFP-TET1CD, and gRNA expression system.DepositorInsertdCas9-5xPlat2AflD-P2A-scFvGCN4sfGFPTET1CD
ExpressionMammalianAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR5'_ubi
Plasmid#27320DepositorInsertzebrafish ubiquitin promoter (ubb Zebrafish)
UseMultisite gateway 5' entry vectorAvailable sinceFeb. 3, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
murine TCR OTI-2A.pMIG II
Plasmid#52111Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorPublicationUseRetroviralTagsT2A (from Thosea asigna virus)ExpressionMammalianAvailable sinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1_Puro_Tet3G_3xFLAG_Twin_Strep
Plasmid#92099PurposeDoyon lab Tandem-Affinity Purification Following Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus. Transgene expression is controlled by an Auto-Regulated Tet-On 3G System.DepositorTypeEmpty backboneUseCRISPR and TALEN; ZfnExpressionMammalianPromoterTet-On 3G BidirectionalAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK287 (mAID-Hygro)
Plasmid#72825PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Hygro
UseCRISPRTagsmAIDExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAVS1-Neo-M2rtTA
Plasmid#60843PurposeAAVS1 donor vector for genomic targetingDepositorInsertsM2rtTA
Neo
UseTargeting donorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pU6-sgRosa26-1_CBh-Cas9-T2A-BFP
Plasmid#64216PurposeExpression vector for a sgRNA against the mouse Rosa26 locus and Cas9 linked to BFP via a T2A peptideDepositorInsertsCas9
sgRNA targeting ROSA26-1
UseCRISPRTags3xFLAG, NLS, and T2A-BFPExpressionMammalianPromoterCBh and U6Available sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-NDi-CRISPRi (Gen1)
Plasmid#73497PurposeDox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry markerDepositorInsertsdCas9-KRAB-P2A-mCherry
rtTA
UseCRISPRTagsHA, KRAB, and NLSExpressionMammalianMutationD10A, H840A (catalytically deactivated Cas9)PromoterCAG and TRE3GAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR
Plasmid#60231PurposeExpresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.DepositorInsertssgRNA
Cre recombinase
EGFP
UseAAV, CRISPR, Cre/Lox, and Mouse TargetingTagsCre-HA-P2A and EGFP-KASHExpressionMammalianPromoterU6 and hSynAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTR
Plasmid#63143PurposePCR template to construct polycistronic tRNA-gRNADepositorInsertgRNA-tRNA fusion
UseCRISPR and Synthetic BiologyAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
Plasmid#67974PurposeCRISPR gRNA expression vector with an improved scaffold and puro/BFP markersDepositorInsertU6gRNA cassette, PGKpuro2ABFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by