We narrowed to 5,559 results for: Chia
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Plasmid#30100DepositorInsertpLtetO-1:DsrA, pLlacO-1:rpoS::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3859 (=HL 716 + pHL 1305)
Plasmid#30089DepositorInsertpLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3450 (=HL 3262 + pHL 1191)
Plasmid#30080DepositorInsertpLlacO-1:OxyS-mCherry, pLtetO-1:RBS (st7) gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3449 (=HL 3262 + pHL 1179)
Plasmid#30079DepositorInsertpLtetO-1:mCherry, pLlacO-1:fhlA::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2304 (=HL 716 + pHL 269)
Plasmid#30019DepositorInsertpLtetO-1:MicC-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2300 (=HL 716 + pHL 745)
Plasmid#30017DepositorInsertpLtetO-1:RhyB-mCherry, pLlacO-1:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 1178 (=HL 862 + pHL 282)
Plasmid#30012DepositorInsertpLtetO-1:mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceSept. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 4178 (=HL 862 + pHL 1360)
Plasmid#30097DepositorInsertpLlacO-1:hfq, pLtetO-1:ompC::gfp
UseSynthetic BiologyTagsGFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2314 (=HL 716 + pHL 818)
Plasmid#30025DepositorInsertpLtetO-1:MicC, pLtetO-1:fhlA::mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 2625 (=HL 716 + pHL 908)
Plasmid#30028DepositorInsertpLlacO-1:RhyB::mCherry, pLtetO-1m9:sodB::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3612 (=HL 716 + pHL 841)
Plasmid#30082DepositorInsertpLtetO-1:RhyB-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
HL 3619 (=HL 716 + pHL 1228)
Plasmid#30083DepositorInsertpLtetO-1:DsrA-mCherry, pLlacO-1:ompC::gfp
UseSynthetic BiologyTagsmCherry, GFPExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSL7 SMR1791
Plasmid#28030DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCMV Ub Lex VP16 (F442A) HA (P#1711)
Plasmid#14596DepositorInsertLexA
UseTagsHA, Ubiquitin, and VP16 (F442A)ExpressionMammalianMutationThe VP16 sequence contains an F442A mutation. The…PromoterAvailable sinceJune 30, 2008AvailabilityAcademic Institutions and Nonprofits only -
3XFlag-pA-Tn5-Fl
Plasmid#124601PurposeExpresses protein A and Tn5 Transposase fusion protein in bacterial cellsDepositorInsertProtein A and hyperactive Tn5 transposase (Tnp) fusion protein
UseTagsProtein A and Tn5 transposase fusion protein with…ExpressionBacterialMutationPromoterT7Available sinceMay 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-LYC
Plasmid#53270PurposeContains 3 genes (crtE, crtI, and crtB) of the carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces lycopene in Escherichia coliDepositorInsertcrtE, crtI, crtB
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein
Plasmid#117847PurposeBioBrick pSB1C3 plasmid that constitutively overexpresses amilCP chromoprotein in E. coliDepositorInsertpromoter, RBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein promoter-less
Plasmid#118008PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilCP
UseSynthetic Biology; Escherichia coliTagsExpressionMutationPromoterAvailable sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx120-20xMS2-Hyg
Plasmid#210220PurposeIntegrates 120xLacO repeats and 20xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts120xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR-PsppA-mCherry
Plasmid#225428PurposePlasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherryDepositorInsertmCherry, Cas9, SgRNA
UseCRISPRTagsExpressionBacterialMutationPromoterPsppA upstream of mCherry and Cas9, P3 upstream o…Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCutamp
Plasmid#140632PurposePlasmid-curing in Escherichia coli by targeting the AmpR promoterDepositorInsertSpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANAP
Plasmid#48696PurposeAllows co-translational incorporation of the fluorescent ncAA Anap directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115 and T7/lacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC-EHER
Plasmid#53262PurposeContains a 7 gene carotenoid pathway gene cluster of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin diglucoside in Escherichia coliDepositorInsertcrtE, idi, crtX, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBS-U6-crRNA-empty
Plasmid#134921PurposeComponents for genome editing in mammalian cells with pPB-CAG-hCas3 and pCAG-All-in-one-hCascade.DepositorInsertcrRNA for CRISPR-Cas3
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSCM001
Plasmid#124605PurposeExpresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pHDepositorInsertmCherry-pHluorin
UseTagsmCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…ExpressionBacterialMutationPromoterpDABAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-tTA
Plasmid#193338PurposeAAV-mediated expression of tTA under the CAG promoter in mammalian cellsDepositorInserttTA2
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available sinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
UseTagsExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7Available sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-A
Plasmid#177811PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEL2319
Plasmid#195697PurposeCryo-EM of 6xHis-Foldon-(Gly-Ser-Linker)-MlaCDepositorInsertFoldon-MlaC
UseTagsExpressionBacterialMutation6xHis-Foldon-(Gly-Ser-Linker)-MlaCPromoterAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-SCR-sgRNA-B
Plasmid#177812PurposeOverexpression of sgRNAs in E. coli HT115 (scrambled targeting sequences)DepositorInsertScrambled sgRNA targeting sequences
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
aav-CAG-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113761PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the CAG promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterCAGAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
PVS10
Plasmid#104398Purposepolycistronic vector for expression of E. coli core RNAPDepositorUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRetroX-PTuner DD-linker-M.EcoGII
Plasmid#122082PurposeExpress M.EcoGII with detabilization domain - inducibleDepositorInsertDD-linker-M.EcoGII
UseRetroviralTagsV5 tag on Nter of M.EcoGIIExpressionBacterial and MammalianMutationPromoterAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21-Flavidin
Plasmid#89881PurposeFluorophore-friendly streptavidin that can improve fluorescent brightness and has unimpaired biotin binding after dye labelingDepositorInsertStreptavidin-K80R-K121R-K132R-K134R-Glutamate_Tag
UseTags6 glutamate tag (C terminal on insert)ExpressionBacterialMutationK80R, K121R, K132R, K134RPromoterAvailable sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCJR01
Plasmid#187888PurposeEscherichia coli plasmid with cloned regions of homology to Campylobacter jejuni chromosome flanking a NotI restriction site. pCJR01 functions as a suicide vector for integrating cloned NotI DNA.DepositorInsertDNA insert has homology to a rRNA region of the Campylobacter jejuni chromosome with an added central NotI restriction site
UseTagsExpressionBacterialMutationPromoternoneAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1651-sgRNA(F+E)-sgGal4
Plasmid#100549PurposesgGal4-expressing vector modified from Addgene plasmid #51024DepositorInsertsgGal4
UseCRISPRTagssgRNAExpressionMammalianMutationPromoterU6Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available sinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNP2270
Plasmid#222733PurposepNP1950 - J23119-IS621 bRNA1 TBL-lacZ-1 (Extended RTG), DBL-WT, HDV; T7-IS621 RecombinaseDepositorInsertIS621 Helper (lacZ)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pORTMAGE312B
Plasmid#128969PurposeBroad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid inductionDepositorInsertsPapRecT
mutL E32K
UseTagsExpressionBacterialMutationE32KPromoterxylS-PmAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMG343
Plasmid#176183Purposepreassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 markerDepositorInsertTPK2+ccdB dropout cassette
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
prDNA spacer-LacOx50-20xMS2-Hyg
Plasmid#210219PurposeIntegrates 50xLacO repeats and 24xMS2 repeats with a hygromycin drug selection marker into the rDNA spacer.DepositorInserts50xLacO repeats derived from: PMID: 12864855.
24xMS2 repeats derived from Addgene plasmid # 31865
UseTagsExpressionMutationPromoterAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLaNGo11-7
Plasmid#126055PurposeA self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cellDepositorInsertschloroplastic FNR transit peptide
ccdB cassette (BsaI)
GFP11x7 (5 aa linker)
UseBinaryTagsGFP1-10ExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGM1202
Plasmid#69615Purposeinducible gene expression in Streptomyces, C-terminal His-tag encoding sequenceDepositorInsertsaac
tsr
rep
sso
TipA promoter
dso
UseTagsExpressionMutationPromoterAvailable sinceJan. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
UseTagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EPromoterAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAT253
Plasmid#40943DepositorInsertGFP-LacI**
UseTagsGFP(S65T, V163A) and SV40 NLSExpressionYeastMutationLacI**/LacI2G(4 mutations) (see comment below); l…PromoterHis3 promoter fragmentAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only