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We narrowed to 5,870 results for: RIK

Showing: 5001 - 5050 of 5870 results
  1. pB80-KIF1A(1-383)-GFP-SSPB(milli)

    Plasmid
    #174626
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(milli)
    Depositor
    Insert
    KIF1A(1-383)-GFP-SSPB(milli) (Kif1a Synthetic, Mouse)
    Use
    Tags
    GFP-SSPB(milli)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
  2. pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G

    Plasmid
    #113953
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant
    Depositor
    Insert
    T1R2 (TAS1R2 Human)
    Use
    Tags
    FLAG and Signal/leader sequence from influenza he…
    Expression
    Mammalian
    Mutation
    delete N-terminal 21 residues, I132S, S212N, and …
    Promoter
    cmv
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pOD2087-trnDEG

    Plasmid
    #89369
    Purpose
    MosSCI targeting vector expressing a fusion between a GFP nanobody and an ubiquitin ligase adaptor ZIF-1 to degrade GFP tagged proteins. Expression is controlled by Pmec-18 (touch neuron specific).
    Depositor
    Insert
    vhhGFP4-ZIF-1 (zif-1 Synthetic, Nematode)
    Use
    Targeting vector for mos1 transposon mediated sin…
    Tags
    vhhGFP4 (GFP nanobody)
    Expression
    Mutation
    Promoter
    Pmec-18
    Available since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pAR69_STRI_m(KS_MAT)_H8_pET22b

    Plasmid
    #122849
    Purpose
    Expresses the condensing part of murine type I fatty acid synthase (FASN) consisting of the KS and MAT domain in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.
    Depositor
    Insert
    m(KS_MAT) (Fasn Mouse)
    Use
    Tags
    His8 tag and Twin-Strep tag
    Expression
    Bacterial
    Mutation
    wildtype
    Promoter
    T7 promoter
    Available since
    March 19, 2019
    Availability
    Academic Institutions and Nonprofits only
  6. pCDNA3.1-nAChr-beta3

    Plasmid
    #48811
    Purpose
    This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.
    Insert
    nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    *
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. RCAS-sgATG7

    Plasmid
    #75228
    Purpose
    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh
    Depositor
    Insert
    Autophagy Related 7 (Atg7 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    U6
    Available since
    June 2, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. pB80-KIF1A(1-383)-GFP-SSPB(nano)

    Plasmid
    #174628
    Purpose
    Constitutively active KIF1A for optogenetic heterodimerization to iLID via SSPB(nano)
    Depositor
    Insert
    KIF1A(1-383)-GFP-SSPB(nano) (Kif1a Synthetic, Mouse)
    Use
    Tags
    GFP-SSPB(nano)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-383): Pro202Ala; EGFP: Met1Del;
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  9. pB80-KIF1A(1-365)-GFP-SSPB(micro)

    Plasmid
    #174629
    Purpose
    Non-processive monomeric KIF1A for optogenetic heterodimerization to iLID via SSPB(micro)
    Depositor
    Insert
    KIF1A(1-365)-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    Use
    Tags
    GFP-SSPB(micro)
    Expression
    Mammalian
    Mutation
    mmKIF1A(aa1-365): Pro202Ala; EGFP: Met1Del; SSPB:…
    Promoter
    Chicken beta-actin
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
  10. pIRES:mTREK-1(K271Q)

    Plasmid
    #133273
    Purpose
    Mammalian expression vector. It will generate the mouse TREK-1 channel full lenght K271Q mutant fused to a N-terminal HA tag
    Depositor
    Insert
    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian and Yeast
    Mutation
    K271Q
    Promoter
    Available since
    April 27, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. psicheck-2-LPPRC

    Plasmid
    #48167
    Purpose
    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element
    Depositor
    Insert
    LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Oct. 16, 2013
    Availability
    Academic Institutions and Nonprofits only
  12. pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

    Plasmid
    #58414
    Purpose
    Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells
    Insert
    IFITM3 (Ifitm3 Mouse)
    Use
    Tags
    HA internal
    Expression
    Mammalian
    Mutation
    Cysteines 71, 72, and 105 to Alanine; The sequenc…
    Promoter
    CMV IE
    Available since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5001 - 5050 of 5870 results