We narrowed to 17,154 results for: REV

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pCEV-G4-Km

Plasmid

#46819

Purpose

TEF1 and HXT7 promoter controlled expression cassettes with G418 resistance for yeast transformation

Depositor

Lars Nielsen, Claudia Vickers

Article

Vickers et al Microb Cell Fact. 2013 Oct 25;12(1):96.

Type

Empty backbone

Tags

FLAG and c-myc

Expression

Yeast

Promoter

TEF1 and HXT7

Available Since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pTYF-mCMV/SYN-EGFP

Plasmid

#19975

Depositor

Sergey Kasparov

Article

Liu et al BMC Biotechnol. 2008 May 16;8:49. doi: 1

Insert

mCMV/SYN-EGFP

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO APEX2-GFP

Plasmid

#129640

Purpose

Express APEX2-GFP fusion in mammalian cells

Depositor

Nicholas Ingolia

Article

Padron et al Mol Cell. 2019 Aug 22;75(4):875-887.e5.

Insert

APEX2-GFP

Expression

Mammalian

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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NES-EGFP-PH-ARNO2G-I303Ex2

Plasmid

#116868

Purpose

PIP3 biosensor

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

CYTH2 (CYTH2 Human)

Tags

EGFP and X.leavis map2k1.L(32-44)

Expression

Mammalian

Mutation

amind acids 252-399, isoleucine 303 changed to gl…

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLncEXP

Plasmid

#64865

Purpose

Empty vector for lncRNA expression

Depositor

Lei Sun

Article

Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

Type

Empty backbone

Use

Retroviral

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only
β₂AR-mCFP

Plasmid

#38260

Depositor

Robert Lefkowitz

Article

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Insert

β₂AR (ADRB2 Human)

Tags

Flag, mCFP, and signal peptide sequence (MKTIIALS…

Expression

Mammalian

Mutation

diglycine linker (replacing terminator codon)

Promoter

CMV

Available Since

Aug. 23, 2012

Availability

Academic Institutions and Nonprofits only
pJC53.2

Plasmid

#26536

Purpose

T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes

Depositor

Phillip Newmark

Article

Collins et al PLoS Biol. 2010 . 8(10):e1000509.

Type

Empty backbone

Use

T/a cloning vector for dsrna generation and the g…

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pYES2-Ub-G76V-GFP

Plasmid

#11954

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-G76V-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Glycine 76 …

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pPAICS-EGFP

Plasmid

#99108

Purpose

Expresses human PAICS-EGFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

PAICS (PAICS Human)

Tags

EGFP (enhanced green fluorescent protein)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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3537 pMIG Bim

Plasmid

#8786

Depositor

Stanley Korsmeyer

Article

Cheng et al Mol Cell 2001 Sep;8(3):705-11.

Insert

Bim (Bcl2l11 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

Plasmid

#154786

Purpose

Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE

Depositor

Robert Singer

Article

Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:

Insert

Adenosine deaminse (Adar Synthetic, Fly)

Use

Lentiviral

Tags

GFP, V5, and mCherry

Expression

Mammalian

Mutation

Synonymized serine to prevent autoediting of ADAR…

Promoter

UBC

Available Since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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FUW-TetO-Zeo-Ezh2

Plasmid

#40794

Depositor

Rudolf Jaenisch

Article

Buganim et al Cell. 2012 Sep 14;150(6):1209-22. doi: 1

Insert

Ezh2 (Ezh2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Minimal CMV + TetO

Available Since

Oct. 12, 2012

Availability

Academic Institutions and Nonprofits only

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TRE:mCherry-p2a- bReaChES

Plasmid

#163037

Purpose

FLiCRE reporter gene for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

mCherry-p2a-bReaChES

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJH136

Plasmid

#47554

Purpose

pJH136 carries a URA3 cassette flanked by PCR priming sites U2 and D2. URA3 was derived from S288c and extends from 243 bp upstream of the start codon to 79 bp downstream of the stop codon.

Depositor

Ronald Davis

Article

Horecka et al Yeast. 2014 Mar;31(3):103-12. doi: 10.10

Insert

URA3

Use

Pcr template

Promoter

Native URA3 promoter, -243 to -1.

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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TOM20*-SBP-GFP

Plasmid

#120173

Purpose

Expresses a chimera of the mitochondria-targeting signal of TOM20, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Mitochondrial import receptor subunit TOM20 homolog (TOMM20 Human)

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Truncated TOM20: 1-30 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-His-tag-hphMX4

Plasmid

#20764

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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pCCL-GKL1ShortMutPRC-Ug1Lg2starTGA

Plasmid

#52347

Purpose

Integration cassette to create p1-Short-UL*(TGA)

Depositor

Chang Liu

Article

Ravikumar et al Nat Chem Biol. 2014 Mar;10(3):175-7. doi

Insert

leu*

Use

P1 integration vector

Expression

Bacterial

Mutation

changed S2F, A3V, Q180*

Promoter

UCS from pGKL2 ORF5

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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phage UbiC NLS HA stdMCP Halo

Plasmid

#104098

Purpose

Lentiviral vector expressing synonymous tandem MS2 coat protein fused to Halo. Used to label reporter mRNAs.

Depositor

Jeffrey Chao

Article

Horvathova et al Mol Cell. 2017 Nov 2;68(3):615-625.e9. d

Insert

stdMCP-Halo

Use

Lentiviral

Tags

C-terminal Halo-tag, N-terminal NLS, and Two syno…

Expression

Mammalian

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pProEx-IDE-wt

Plasmid

#99014

Purpose

Express human Insulin Degrading Enzyme (IDE) in E. coli.

Depositor

Wei-Jen Tang

Article

Shen et al Nature. 2006 Oct 19;443(7113):870-4. Epu

Insert

insulin degrading enzyme (IDE Human)

Tags

His tag

Expression

Bacterial

Promoter

TRC

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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