19,627 results

1. pET28a-sfGFP

   Plasmid

   #85492

   Purpose

   pET28a expressing codon optimized superfolder GFP with C-terminal His6 tag

   Depositor

   Ryan Mehl

   Article

   Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

   Insert

   superfolder GFP
   

   Tags

   6x His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS_VP35_EBOV

   Plasmid

   #103050

   Purpose

   Encodes Ebola virus VP35 gene (Zaire ebolavirus, Mayinga) under control of the CAG promoter

   Depositor

   Elke Mühlberger

   Article

   Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

   Insert

   VP35 gene of Ebola virus (VP35 (Zaire ebolavirus, Mayinga))
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #46295

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.18 CMV-Luc

   Plasmid

   #100984

   Purpose

   CMV luciferase reporter - firefly luciferase driven by CMV promoter

   Depositor

   Lee Helman

   Article

   Grohar et al J Natl Cancer Inst. 2011 Jun 22;103(12):

   Insert

   CMV
   

   Expression

   Mammalian

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSGV-1D3-28Z All ITAMs intact

   Plasmid

   #107226

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs intact

   Depositor

   Steven Rosenberg, James Kochenderfer

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z All ITAMs intact
   

   Use

   Retroviral

   Tags

   None

   Mutation

   None

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTRIP-CMV-tagRFP-FLAG-cGAS

   Plasmid

   #86676

   Purpose

   Expresses catalytically-active tagRFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

   Depositor

   Nicolas Manel

   Article

   Raab et al Science. 2016 Apr 15;352(6283):359-62. d

   Insert

   tagRFP-FLAG-cGAS
   

   Use

   Lentiviral

   Available Since

   April 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mOrange2-pBAD

   Plasmid

   #54531

   Purpose

   Localization: Protein Expression Vector , Excitation: 549, Emission: 565

   Depositor

   Michael Davidson, Roger Tsien, Nathan Shaner

   Article

   Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

   Type

   Empty backbone

   Tags

   6xHis and mOrange2

   Expression

   Bacterial

   Available Since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Flag-Gsdmd

   Plasmid

   #80950

   Purpose

   Expresses Gsdmd in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

   Insert

   Gsdmd (Gsdmd Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Aug. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. VPR-ps-dSpCas9

   Plasmid

   #102855

   Purpose

   A single-chain light-controllable dSpCas9 with pdDronpa1.2 domains

   Depositor

   Michael Lin

   Article

   Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

   Insert

   dSpCas9, pdDronpa1.2
   

   Use

   CRISPR and Synthetic Biology

   Tags

   NLS and VP64-p64-Rta transcription activator

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Puro-dTAG

    Plasmid

    #207694

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-dTAG cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pCF525-mTagBFP2_EF1a-Hygro-P2A-mTagBFP2_lenti

    Plasmid

    #138315

    Purpose

    Lenti expression of mTgBFP2

    Depositor

    Jennifer Doudna

    Article

    Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

    Insert

    mTAGBFP2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    March 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223-JAK2

    Plasmid

    #23915

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    JAK2 (JAK2 Human)
    

    Use

    Gateway donor vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. LV-Cre pLKO.1

    Plasmid

    #25997

    Purpose

    Lentiviral expression of nls-Cre from the CMV promoter

    Depositor

    Elaine Fuchs

    Article

    Beronja et al Nat Med. 2010 Jul . 16(7):821-7.

    Insert

    Cre recombinase
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSpCas9_T2A_iRFP670 sgAAVS1

    Plasmid

    #192477

    Purpose

    All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

    Depositor

    Michael Yaffe

    Article

    van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

    Insert

    SpCas9
    

    Tags

    T2A iRFP670

    Expression

    Mammalian

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hsyn-ASAP5-WPRE

    Plasmid

    #225709

    Purpose

    Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5
    

    Use

    AAV

    Promoter

    human synapsin

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_118

    Plasmid

    #133459

    Purpose

    U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR

    Depositor

    John Doench

    Article

    DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

    Insert

    control guide
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pINDUCER21 (ORF-EG)

    Plasmid

    #46948

    Purpose

    Inducible lentiviral gene expression vector. Chloramphenicol cm + ccdB cassette may be removed to clone in gene of interest

    Depositor

    Stephen Elledge, Thomas Westbrook

    Article

    Meerbrey et al Proc Natl Acad Sci U S A. 2011 Mar 1;108

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLentiPGK Puro DEST JNKKTRClover

    Plasmid

    #59151

    Purpose

    Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    JNK Kinase Translocation Reporter (MAPK8 Human, Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Sept. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.10-VEGFprom(-1000 to -1)

    Plasmid

    #66128

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Promoter

    VEGF -1000 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSMPP-mCherry-hTRIM21

    Plasmid

    #104972

    Purpose

    Lentiviral Transfer Plasmid with mCherry-tagged human TRIM21

    Depositor

    Leo James

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    Tripartite Motif containing 21 (TRIM21 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Promoter

    SFFV

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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