We narrowed to 11,516 results for: Kars

Showing: 4121 - 4160 of 11516 results

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pAAV.GFAP.(mem).iGlucoSnFR2

Plasmid

#244088

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pTET2-RlucCP

Plasmid

#198352

Purpose

Part of Firefly reporter system, encodes Renilla luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Renilla Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag_fast

Plasmid

#167275

Purpose

Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

Depositor

Kai Johnsson

Article

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(mem).iGlucoSnFR2

Plasmid

#244077

Purpose

Plasma membrane targeted expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Tags

IgG kappa leader

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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p27-IRES-mCherry

Plasmid

#80143

Purpose

retroviral expression of p27 and mCherry

Depositor

Ellen Rothenberg

Article

Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

Insert

p27 (CDKN1B Human)

Use

Retroviral

Tags

IRES-mCherry

Expression

Mammalian

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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EF1a_MYCN_P2A_Hygro_Barcode

Plasmid

#120463

Purpose

Barcoded lentiviral vector to express MYCN in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYCN (MYCN Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pC001 - huLshC2C2-MBP for bacterial expression

Plasmid

#79150

Purpose

Expresses human codon-optimized LshC2c2 for purification in E. coli

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Insert

C2c2

Expression

Bacterial

Promoter

T7

Available Since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.mRuby3

Plasmid

#244106

Purpose

Bacterial expression of green glucose sensor with inert mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Tags

mRuby3

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pBMN-HA-TBK1

Plasmid

#199205

Purpose

Used for the expression of TBK1 (SMC Internal No. 1996)

Depositor

Sascha Martens

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

TBK1 (TBK1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-LC3-YFP

Plasmid

#99571

Purpose

Lentiviral expression of YFP-tagged LC3

Depositor

Edward Campbell

Article

Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

Insert

LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEF1a-hMLH1NTD-NLS

Plasmid

#174826

Purpose

Mammalian expression of human MLH1NTD-NLS (codon optimized)

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

human MLH1_NTD-NLS (codon optimized)

Tags

SV40 bpNLS

Expression

Mammalian

Mutation

truncation of residues 1-335

Promoter

EF1a

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTEF1

Plasmid

#46922

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgTEF1 promoter (TEF1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Promoter

SFFV

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

Plasmid

#172506

Purpose

Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

Insert

DisCas7-11 bacterial expression vector with golden gate site for guides

Expression

Bacterial

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER-mARG_ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Plasmid

#221080

Purpose

SEMPER plasmid for expression of gas vesicles using mammalian acoustic reporter genes (mARGs)

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-gvpA_ACC-gvpNJKFGW-EmGFP_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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RBM22

Plasmid

#155889

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

RBM22 (RBM22 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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EF1a_NEUROD1_P2A_Hygro_Barcode

Plasmid

#120466

Purpose

Barcoded lentiviral vector to express NEUROD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

NEUROD1 (NEUROD1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCCM’

Plasmid

#162709

Purpose

pCCM reconstructed after selection for CCMB1 growth on glycerol under ambient air

Depositor

David Savage

Article

Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

Insert

Second CCM operon cloned from H. neapolitanus

Expression

Bacterial

Mutation

p15A origin replaced with high-copy colE1 origin

Promoter

PLtet0-1 promoter

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pHS0812 hU6-BpiI-OgeuIscB omegaRNA scaffold

Plasmid

#176541

Purpose

human U6-driven expression of OgeuIscB omegaRNA scaffold with BpiI site for guide cloning

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

OgeuIscB omegaRNA scaffold

Expression

Mammalian

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pY001 (pFnCpf1_full)

Plasmid

#69973

Purpose

Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

FnCpf1 locus

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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spisPink chromoprotein

Plasmid

#117839

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses spisPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, spisPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
amilCP_Pink chromoprotein

Plasmid

#117851

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Pink

Use

Synthetic Biology; Escherichia coli

Mutation

64A,65C, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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gfasPurple chromoprotein

Plasmid

#117849

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, gfasPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAB1678 CMV-HIVNES-GS-Cas13bt1

Plasmid

#176316

Purpose

CMV-HIVNES-GS-Cas13bt1 (human codon optimized Cas13bt1 expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

human codon optimized Cas13bt1

Use

CRISPR

Tags

HIV NES

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV_hU6-sgRNA_hUbC-GFP-P2A-PuroR

Plasmid

#162335

Purpose

Lentiviral expression of S. pyogenes sgRNA with a GFP-P2A-PuroR selection marker

Depositor

Charles Gersbach

Article

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

S. pyogenes sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
amilGFP chromoprotein

Plasmid

#117842

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilGFP chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilGFP

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTET2-lucCP

Plasmid

#198353

Purpose

Part of Renilla reporter system, encodes Firefly luciferase

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

Firefly Luciferase

Use

Luciferase

Expression

Mammalian

Promoter

pTET2

Available Since

April 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
amilCP_Orange chromoprotein

Plasmid

#117850

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses amilCP_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, amilCP_Orange

Use

Synthetic Biology; Escherichia coli

Mutation

64V,65G, BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
asPink chromoprotein

Plasmid

#117838

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, asPink

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NSUN2

Plasmid

#155793

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NSUN2 (NSUN2 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT166_GalL_A3A-BE3

Plasmid

#145116

Purpose

Expressing base editor A3A-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

A3A-BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MEF2D-2A-neo

Plasmid

#190622

Purpose

Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

Depositor

Junwei Shi

Article

Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

Insert

MEF2D (MEF2D Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
RBD-Fc

Plasmid

#164221

Purpose

Expresses soluble , dimeric Fc-His fusion protein for the original SARS-CoV2 Spike RBD region, along with dTomato reporter

Depositor

Sriram Neelamegham

Article

Yang et al Elife. 2020 Oct 26;9. pii: 61552. doi: 1

Insert

Spike RBD (S SARS-CoV)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
SMG6

Plasmid

#156020

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SMG6 (SMG6 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
SUPV3L1-HA G207V

Plasmid

#232349

Purpose

Expresses human SUPV3L1/SUV3 (G207V) in mammalian cells with HA tag

Depositor

Samantha Lewis

Article

Begeman et al Sci Adv. 2025 Apr 18;11(16):eads6830. do

Insert

SUPV3L1 (SUPV3L1 Human)

Tags

HA

Expression

Mammalian

Mutation

G207V

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pST1374-N-NLS-flag-linker-Cas9-D10A

Plasmid

#51130

Purpose

Cas9 nickase optimized for nuclear import.

Depositor

Xingxu Huang

Article

Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

Insert

Cas9 nickase

Use

CRISPR

Tags

Flag and NLS

Expression

Mammalian

Mutation

D10A (nickase mutant)

Promoter

CMV

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBMN HA-NDP52

Plasmid

#199307

Purpose

For expression of the mammalian NDP52 tagged with HA

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Insert

NDP52 (CALCOCO2 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-Flag-hOct4

Plasmid

#20072

Depositor

Juan Belmonte

Article

Aasen et al Nat Biotechnol. 2008 Nov . 26(11):1276-8

Insert

hOct4 (POU5F1 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
EF1a_OTX2_P2A_Hygro_Barcode

Plasmid

#120471

Purpose

Barcoded lentiviral vector to express OTX2 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

OTX2 (OTX2 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 11,516 results for: Kars