We narrowed to 11,246 results for: Kars

Showing: 4121 - 4160 of 11246 results

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DCT.VV.Orf45

Plasmid

#12733

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf45

Expression

Mammalian

Available Since

Dec. 1, 2006

Availability

Academic Institutions and Nonprofits only
pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pHS1871

Plasmid

#205976

Purpose

U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH DR-BpiI-DR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 2, 2024

Availability

Academic Institutions and Nonprofits only

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Psicheck2 COLIA1 full length 3'UTR

Plasmid

#26993

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

COL1A1 Full length 3' UTR (COL1A1 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Feb. 16, 2011

Availability

Academic Institutions and Nonprofits only
pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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DHFR-GFP-th-NL1

Plasmid

#173007

Purpose

For light regulated release of neuroligin 1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and NL1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-NL1

Tags

DHFR and EGFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PLAGL1

Plasmid

#61542

Purpose

Expresses PLAGL1 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

PLAGL1 (PLAGL1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
PRPF18

Plasmid

#155846

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

PRPF18 (PRPF18 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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THOC3

Plasmid

#156082

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC3 (THOC3 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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tsPurple chromoprotein promoter-less

Plasmid

#118007

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

RBS, tsPurple

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pBZcsx27

Plasmid

#89900

Purpose

Expresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoter

Depositor

Feng Zhang

Article

Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

Insert

csx27

Expression

Bacterial

Promoter

Lac

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pHS1517

Plasmid

#205980

Purpose

FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas5-HNH

Tags

FLAG-SV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pBlastR-12xLexAOp-mCherry-MiniWhite

Plasmid

#165917

Purpose

Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Selectable LexAOp Response Vector

Use

Synthetic Biology

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHL

Plasmid

#79614

Purpose

Expresses dCas9-SH3 and PmCDA1-SHL in yeast cells

Depositor

Akihiko Kondo

Article

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Inserts

SpCas9
PmCDA1

Tags

SH3 domain and SHL

Expression

Yeast

Mutation

D10A and H840A for nuclease deficient Cas9

Promoter

pGal1 and pGal10

Available Since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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NUDT16L1

Plasmid

#155795

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NUDT16L1 (NUDT16L1 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-Hes5

Plasmid

#61536

Purpose

Expresses Hes5 in mammalian cells

Depositor

Rudolf Jaenisch

Article

Cassady et al Stem Cell Reports. 2014 Nov 6. pii: S221

Insert

Hes5 (Hes5 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2015

Availability

Academic Institutions and Nonprofits only
VVT1

Plasmid

#65779

Purpose

The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SaCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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MSP2253

Plasmid

#70704

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

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PVT1-Tol2

Plasmid

#113381

Purpose

evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

Depositor

Ralf Jauch

Article

Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

Insert

PVT1-enhancer (PVT1 Human)

Use

Tol2 reporter

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only
pCI-MS2V5-PABPC1 MLLEmut M161A/D165K

Plasmid

#65810

Purpose

expression of PABPC1-MS2 fusion, double mutant.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PABPC1 (PABPC4 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Mutation

MLLEmut M161A/D165K

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Tec-SH2

Plasmid

#46522

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Tec (TEC Human)

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 236-351

Promoter

Tac

Available Since

Oct. 9, 2013

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf106

Plasmid

#12794

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf106

Expression

Mammalian

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
EF1a_SIX1_P2A_Hygro_Barcode

Plasmid

#120476

Purpose

Barcoded lentiviral vector to express SIX1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

SIX1 (SIX1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSMP-SUZ12_1

Plasmid

#36390

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SUZ12 (SUZ12 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
THOC5

Plasmid

#156084

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

THOC5 (THOC5 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSMP-Dot1L_2

Plasmid

#36340

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Dot1L (DOT1L Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pHDR_NANOS3-T2A-mVenus-PuroTK

Plasmid

#222903

Purpose

Homology directed repair template for knocking in mVenus reporter to NANOS3.

Depositor

George Church

Article

Pierson Smela et al bioRxiv 2022.07.11.499564

Insert

mVenus

Use

CRISPR

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX Stat6-SH2

Plasmid

#46517

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat6 (STAT6 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 510-654

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pHS1929

Plasmid

#205968

Purpose

JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

Insert

Cas8-HNH crRNA

Expression

Bacterial

Promoter

T7

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-LKB1(KD)

Plasmid

#84640

Purpose

mCherry-tagged, LKB1(K78I); kinase-dead mutant

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

mCherry-LBK1(K78I) (STK11 Human, Synthetic)

Tags

6xHis, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Mutation

Lysine 78 mutated to Isoleucine; kinase dead

Promoter

CMV

Available Since

May 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSMP-EZH1_2

Plasmid

#36360

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EZH1 (EZH1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pJT25_GalL_BE3

Plasmid

#143736

Purpose

Expresses BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
DHFR-GFP-th-GluA1

Plasmid

#173008

Purpose

For light regulated release of GluA1 from the endoplasmic reticulum. Driven by synapsin promoter. Thrombin site is included between GFP and GluA1.

Depositor

Matthew Kennedy

Article

Bourke et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

DHFR-GFP-th-GluA1

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

human synapsin

Available Since

Aug. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJT82_GalL_nCDA1Δ198-BE3

Plasmid

#145046

Purpose

Expresses nCDA1Δ198-BE3 in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ198-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-198AA); spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pFE-hCA

Plasmid

#132999

Purpose

pFE carrying a human carbonic anhydrase II gene (Uniprot: P00918)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Insert

hCAII (CA2 Human)

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Flag-hsDicer (Y971A/Y972A)

Plasmid

#41590

Depositor

Phil Sharp

Article

Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

Insert

hsDicer (DICER1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Y971A/Y972A

Promoter

CMV

Available Since

Feb. 7, 2013

Availability

Academic Institutions and Nonprofits only
pY005 (pcDNA3.1-hLb3Cpf1)

Plasmid

#69977

Purpose

Expresses humanized Lb3Cpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLb3Cpf1

Use

CRISPR

Tags

NLS & 3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only
pHS0667 KraIscB-1 non-coding locus in pCOLADuet-1

Plasmid

#176537

Purpose

Non-protein coding locus components from KraIscB-1

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

non-coding locus components from KraIscB-1 locus

Expression

Bacterial

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 4121 - 4160 of 11246 results

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We narrowed to 11,246 results for: Kars