We narrowed to 6,925 results for: CAD

1. pET28_6xHis-PARP1-W318R

   Plasmid

   #174793

   Purpose

   Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-W318R (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   W318, V762A

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. J23114-mRFP1-331Bb

   Plasmid

   #78279

   Purpose

   mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23114-mRFP1
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p6589 MSCV-IP N-HAonly CHEK2

   Plasmid

   #34903

   Depositor

   Peter Howley

   Article

   White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

   Insert

   CHEK2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV LTR

   Available since

   Feb. 8, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3/flag-pBirA(1-G78)-FRB

   Plasmid

   #154447

   Purpose

   expresses pBirA(1-G78)-FRB/biochemical assay

   Depositor

   Hyun-Woo Rhee

   Article

   Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

   Insert

   flag-pBirA(1-G78)-FRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMKO shRNA Bmf

   Plasmid

   #17236

   Depositor

   Joan Brugge

   Article

   Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

   Insert

   Bmf shRNA (BMF Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 18, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pCS2 Notch1 LNG-6MT

   Plasmid

   #41738

   Depositor

   Raphael Kopan, Jeffrey Nye

   Article

   Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

   Insert

   Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
   

   Use

   Tags

   6xMyc

   Expression

   Mammalian

   Mutation

   Contains murine Notch-1 leader peptide (aa 1-23) …

   Promoter

   Available since

   Jan. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mKikGR-Tubulin-6

   Plasmid

   #57330

   Purpose

   Localization: Microtubules, Excitation: 507 / 583, Emission: 517 / 593

   Depositor

   Michael Davidson

   Article

   Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

   Insert

   Tubulin (TUBA1B Human)
   

   Use

   Tags

   mKikGR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pET28_6xHis-PARP1-A762V

   Plasmid

   #174795

   Purpose

   Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-A762V (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   A762V

   Promoter

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pONL-C1_Rab11a

   Plasmid

   #65707

   Purpose

   Expresses ONL-Rab11a in mammalian cells

   Depositor

   Yasushi Okada

   Article

   Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Rab11a (Rab11a Mouse)
   

   Use

   Luciferase

   Tags

   ONL

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MSCV N-term HA-FLAG puro BRD7 shRescue

    Plasmid

    #41936

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pET22b-ecDHFR-N23PP/L28F/G51PEKN

    Plasmid

    #118585

    Purpose

    Bacterial expression of E.coli DHFR N23PP, L28F, and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, L28 mutated to F28, G51 mutat…

    Promoter

    T7

    Available since

    Nov. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT2-7xTcf-NLS-ONL-CP

    Plasmid

    #65716

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2-7xTcf-NLS-YNL-CP

    Plasmid

    #65714

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mILK-L207W-EFGP

    Plasmid

    #176900

    Purpose

    mouse ILK with L207W point mutation cloned into pEGFP-N1

    Depositor

    Sara Wickström

    Article

    Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    Integrin-linked kinase (Ilk Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Leucine 207 to Tryptophane

    Promoter

    CNV

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN_TGmiR_Gb2-K

    Plasmid

    #25766

    Purpose

    Entry vector withTRE promoter driving mouse G beta 2 miR30-based shRNA with GFP coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Gb2 miR-shRNA (Gnb2 Mouse)
    

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTSara-R756K

    Plasmid

    #60727

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Promoter

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCEP4-myc-HLA-A3-VN

    Plasmid

    #135506

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. P6601 MSCV-IP N-HAonly GARS

    Plasmid

    #34899

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    GARS (GARS1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pT2-7xTcf-NLS-CNL-CP

    Plasmid

    #65715

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. MO70-hJunctate - EF-mutant

    Plasmid

    #79597

    Purpose

    Transient and retroviral expression

    Depositor

    Yousang Gwack

    Article

    Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

    Insert

    junctate (ASPH Human)
    

    Use

    Retroviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    77DAD79 to 77AAA79

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pGEX-2TK-GST-mEKLF

    Plasmid

    #67836

    Purpose

    Encodes GST fusion to murine EKLF (nucleotides 71-1215)

    Depositor

    James Bieker

    Article

    Zhang et al Proc Natl Acad Sci U S A. 1998 Aug 18;95

    Insert

    EKLF (Klf1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Full-length mEKLF is aa 20-376; amino acid 19 is …

    Promoter

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pGEX-2TK cyclin T1 (1-551) (P#422)

    Plasmid

    #14609

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-551) (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-551

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3 mouse Bmf

    Plasmid

    #17244

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (Bmf Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3.1_SNORD27h_115mut

    Plasmid

    #73068

    Purpose

    expression clone for human mutated SNORD27 (C/D box snoRNA U27)

    Depositor

    Stefan Stamm

    Article

    Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

    Insert

    SNORD27 (SNORD27 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    expression cassette for SNORD27 with AS box from …

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

25. pBabe human Bmf

    Plasmid

    #17240

    Depositor

    Joan Brugge

    Article

    Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

    Insert

    Bmf (BMF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

26. p6596 MSCV-IP-N-HA ADRB2

    Plasmid

    #34895

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Feb. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

27. pET22b-ecDHFR-N23PP/G51PEKN

    Plasmid

    #118583

    Purpose

    Bacterial expression of E.coli DHFR N23PP and G51PEKN mutant

    Depositor

    Stephen Benkovic

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Jun 18;11

    Insert

    DHFR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N23 mutated to P23, G51 mutated to P51, a Pro res…

    Promoter

    T7

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pAdTrack shALAS-1

    Plasmid

    #22748

    Depositor

    Bruce Spiegelman

    Article

    Estall et al Proc Natl Acad Sci U S A. 2009 Dec 29. 1

    Insert

    shALAS-1 (Alas1 Mouse)
    

    Use

    Adenoviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

29. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. J23110-mRFP1-331Bb

    Plasmid

    #78277

    Purpose

    mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23110-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCDF1-ADAM29 (wt)

    Plasmid

    #31143

    Depositor

    Yardena Samuels

    Article

    Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

    Insert

    ADAM29 (ADAM29 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. ADAP1

    Plasmid

    #163905

    Purpose

    Full length mouse ADAP1 for purification from insect cells

    Depositor

    Martin Loose

    Article

    Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    ADAP1 (Adap1 Mouse)
    

    Use

    Tags

    Cleavable 6 x HIS tag

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. GST-CHMP4C (216-233) (pGEX2T(TEV))

    Plasmid

    #80635

    Purpose

    Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

    Depositor

    Wesley Sundquist

    Article

    McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

    Insert

    CHMP4C (CHMP4C Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Residues 216-233

    Promoter

    Available since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCEP4-myc-HLA-A24-VN

    Plasmid

    #135508

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*24:02

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*24:02 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-Myc-XPLN (delta)N (aa1-117 deleted)

    Plasmid

    #73382

    Purpose

    Expresses myc tagged XPLN in mammalian cells with deletion of N-terminal amino acids 1-117

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Use

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Deleted N-terminal 1-117 aa from XPLN protein

    Promoter

    CMV

    Available since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

36. pCMV-Myc-XPLN aa304-466 (PH)

    Plasmid

    #73380

    Purpose

    Expresses amino acids 304-466 of XPLN (Arhgef3) protein

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Use

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Expresses amino acids 304-466 of XPLN (arhgef3) p…

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

37. pCMV-Myc-XPLN aa1-125 (N125)

    Plasmid

    #73378

    Purpose

    Expression of the N-terminal region of XPLN (amino acids 1–125, designated N125)

    Depositor

    Jie Chen

    Article

    Khanna et al Proc Natl Acad Sci U S A. 2013 Oct 1;110

    Insert

    arhgef3 (ARHGEF3 Human)
    

    Use

    Tags

    Myc (QKLISEEDL)

    Expression

    Mammalian

    Mutation

    Expresses N terminal amino acid 1-125 of XPLN (ar…

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

38. His-MBP-NRBF2 (1-159)

    Plasmid

    #99331

    Purpose

    Mammalian expression of the monomeric form of NRBF2 that binds with the PI3K C1 complex

    Depositor

    James Hurley

    Article

    Young et al Proc Natl Acad Sci U S A. 2016 Jul 19;11

    Insert

    Nuclear receptor-binding factor 2 (NRBF2 Human)
    

    Use

    Tags

    6xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    AA1-159 are present

    Promoter

    T7

    Available since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. J23112-mRFP1-331Bb

    Plasmid

    #78278

    Purpose

    mRFP1 behind constitutive promoter J23112 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23112-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. J23103-mRFP1-331Bb

    Plasmid

    #78273

    Purpose

    mRFP1 behind constitutive promoter J23103 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23103-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. J23105-mRFP1-331Bb

    Plasmid

    #78275

    Purpose

    mRFP1 behind constitutive promoter J23105 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23105-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. J23101-mRFP1-331Bb

    Plasmid

    #78272

    Purpose

    mRFP1 behind constitutive promoter J23101 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23101-mRFP1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCEP4-myc-HLA-A1-VN

    Plasmid

    #135502

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*01:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*01:01 (HLA-A Human)
    

    Use

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. WT MetNI in pBAD

    Plasmid

    #118254

    Purpose

    Wild Type Methionine ABC Transporter MetNI in pBAD Vector for Transport Activity Assay

    Depositor

    Douglas Rees

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

    Inserts

    MetN (metN Escherichia coli)
    MetI (metI Escherichia coli)
    

    Use

    Tags

    10x His Tag and Enterokinase Cleavage Site

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose Promoter and None

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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46. pT2-7xTcf-NLS-YNL

    Plasmid

    #65711

    Purpose

    Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    7xTcf, minCMV, 3xNLS, YNL
    

    Use

    Luciferase; Tol2 system

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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47. pCNL-C1_Rab11a

    Plasmid

    #65706

    Purpose

    Expresses CNL-Rab11a in mammalian cells

    Depositor

    Yasushi Okada

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Rab11a (Rab11a Mouse)
    

    Use

    Luciferase

    Tags

    CNL

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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48. pZF-AAA

    Plasmid

    #127465

    Purpose

    coding sequence for wild type zinc finger ZF-AAA (ZiF268)

    Depositor

    Sebastian Maerkl

    Article

    Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

    Insert

    ZF-AAA (EGR1 Human)
    

    Use

    Cell-free transcription–translation

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. pSP64-hERG1a F656A

    Plasmid

    #53054

    Purpose

    Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Phenylalanine 656 to Alanine

    Promoter

    SP6

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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50. pSP64-hERG1a Y652A

    Plasmid

    #53053

    Purpose

    Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Tyrosine 652 to Alanine

    Promoter

    SP6

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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