We narrowed to 12,311 results for: BASE

1. 3xFLAG-dCas9/pMSCVpuro

   Plasmid

   #134983

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Yuno et al Biol Methods Protoc. 2021 Jul 9;6(1):bpa

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. IZ501: pMVP/Lenti-DEST

   Plasmid

   #121848

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. TBP6.7 non-targeting gRNA lambda phage

   Plasmid

   #132547

   Purpose

   expresses gRNA for TBP-based CIRTS

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   gRNA TBP-based CIRTS
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6 promoter

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDM098

   Plasmid

   #216818

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Use

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Promoter

   Available since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

   Plasmid

   #101103

   Purpose

   Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

   Depositor

   Tudor Fulga

   Article

   Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

   Insert

   VEGFR1-dCas9(C)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBT373

   Plasmid

   #125614

   Purpose

   Expresses FERNY-BE4max-NG in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   FERNY-BE4max-NG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-Gal4DBD-GBP2

   Plasmid

   #49439

   Purpose

   Expression of a GFP-dependent transcription factor component in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

   Insert

   GBP2-Gal4 DNA binding domain fusion protein
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. Cenp-B DBD INCENP GFP

   Plasmid

   #45237

   Purpose

   Target INCENP to centromeres via CENP-B DNA binding domain (DBD)

   Depositor

   Michael Lampson

   Article

   Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

   Insert

   Cenp-B-DBD, INCENP-Δcen, GFP
   

   Use

   Tags

   DNA binding domain of CENP-B and GFP

   Expression

   Mammalian

   Mutation

   CEN box deleted from INCENP (aa1-46)

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. P305-FBP_4

   Plasmid

   #139695

   Purpose

   T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

   Depositor

   Daniel Voytas

   Article

   Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

   Insert

   pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
   

   Use

   Luciferase

   Tags

   Expression

   Plant

   Mutation

   Promoter

   various

   Available since

   May 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZJOM144

    Plasmid

    #133630

    Purpose

    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-GFP-HDEL
    

    Use

    Tags

    EGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEN_TRE_GFP

    Plasmid

    #25767

    Purpose

    Entry vector with TRE promoter driving GFP expression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. SLBP non-targeting gRNA lambda phage

    Plasmid

    #132548

    Purpose

    expresses gRNA for SLBP-based CIRTS

    Depositor

    Bryan Dickinson

    Article

    Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

    Insert

    gRNA SLBP-based CIRTS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6 promoter

    Available since

    Sept. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTP924 pHAGE2 CMV GFP

    Plasmid

    #199392

    Purpose

    Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. eSpCas9(1.1)_No_FLAG

    Plasmid

    #79877

    Purpose

    FLAGless construct expressing high specificity eSpCas9(1.1). Px330-like plasmid.

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Untagged eSpCas9(1.1)

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    July 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW-LIC-N8C_Fab3a

    Plasmid

    #203689

    Purpose

    Expresses N8C_Fab3a

    Depositor

    Brenda Schulman

    Article

    Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

    Insert

    N8C_Fab3a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSW002-PpsbA-DsRed-Express2

    Plasmid

    #111257

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PpsbA

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

    Plasmid

    #61255

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-L1-PGK-TKNeo-L2

    Plasmid

    #141008

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    TKNeo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

    Plasmid

    #199356

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBDK

    Plasmid

    #135190

    Purpose

    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    KanR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    cat

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJK511

    Plasmid

    #155384

    Purpose

    dCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    Dec. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUASattB-PKA-SPARK

    Plasmid

    #106922

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSW002-Pc-DsRed-Express2

    Plasmid

    #111251

    Purpose

    Broad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with DsRed-Express2

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PET-3a-chpBK

    Plasmid

    #155201

    Purpose

    To express recombinant ChpBK protein

    Depositor

    Guanzheng Luo

    Article

    Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

    Inserts

    chpS-chpBK-6×His-Tag
    GyrA-CBD-10xHis-Tag
    

    Use

    Tags

    10xHis and 6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pPRIME-CMV-LNGFR-recipient

    Plasmid

    #11660

    Purpose

    3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    LNGFR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSNK

    Plasmid

    #135193

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains kamamycin resistance cassette

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-SceI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-dLbCpf1-BE-YE1

    Plasmid

    #154145

    Purpose

    Expresses dLbCpf1-BE-YE1(dLbCas12a-BE-YE1) in mammalian cells.

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

    Insert

    dLbCpf1-BE-YE1 (dCas12a-BE-YE1)
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y+R126E), dLbCpf1(D832A+E925A+D1148A)

    Promoter

    CMV

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pBlastR-12xLexAOp-mCherry-MiniWhite

    Plasmid

    #165917

    Purpose

    Blasticidin resistant 12xLexAOp mCherry Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable LexAOp Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pcDNA3.nucGAP

    Plasmid

    #78736

    Purpose

    Expression of Ca2+ sensor GAP in the nucleus

    Depositor

    Teresa Alonso

    Article

    Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

    Insert

    nucGAP
    

    Use

    Tags

    nucleoplasmin (Xenopus)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

30. pFA6a-kanMX6-PGAL1

    Plasmid

    #41605

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    GAL1 promoter (GAL1 Budding Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAG-p65AD-GBP6

    Plasmid

    #49440

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP6-p65 transactivation domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBADmod1-linker2-gamS

    Plasmid

    #45833

    Depositor

    Richard Murray, Vincent Noireaux

    Article

    Sun et al ACS Synth Biol. 2014 Jun 20;3(6):387-97.

    Insert

    gamS
    

    Use

    Synthetic Biology

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    May 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCNA

    Plasmid

    #135191

    Purpose

    Arabinose inducible expression of lamba-Red recombinase proteins and I-CreI.

    Depositor

    Hongqing Fang

    Article

    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

    Insert

    I-CreI lamba Red
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    araBAD

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Ccas9LacZ

    Plasmid

    #113083

    Purpose

    Enhanced C. elegans Cas9 vector. Ccas9LacZ contains a lacZ coding sequence, two Cas9 and two type-IIs restriction enzyme recognition sites next to the U6 promoter and gRNA sequences.

    Depositor

    Tibor Vellai

    Article

    Sturm et al Nucleic Acids Res. 2018 Jun 19. pii: 504

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pGEL856

    Plasmid

    #214256

    Purpose

    Expression TadDE-NG in rice cells

    Depositor

    Yong Zhang

    Article

    Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. 3xmNeonGreen-3xrGBD

    Plasmid

    #176101

    Purpose

    Visualization of active Rho

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

    Insert

    rhotekin (Rtkn Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMVdel

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pSaCas9-sgRNA-Tetra-com vector

    Plasmid

    #131227

    Purpose

    For expressing gRNA, whose Tetraloop is replaced with a com aptamer

    Depositor

    Baisong Lu

    Article

    Lyu et al Nucleic Acids Res. 2019 Jul 12. pii: 553

    Insert

    vector for com modified sgRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pYPQ262m3

    Plasmid

    #149556

    Purpose

    Gateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMs

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Plant Commun. 2020 Jul 22;2(2):100101. d

    Insert

    ecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, R221K, N394K, Protospacer interacting domai…

    Promoter

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDEINO4

    Plasmid

    #179488

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    aadA1
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pDEINO3

    Plasmid

    #179487

    Purpose

    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation

    Depositor

    Bogumil Karas

    Article

    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1

    Insert

    TetR/A
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge