We narrowed to 12,311 results for: BASE

1. p667-UBC-CDK11B-V5-miniTurbo_IDG-K

   Plasmid

   #189914

   Purpose

   Gateway destination clone of CDK11B (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK11B (CDK11B Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKF257

   Plasmid

   #52533

   Purpose

   expresses I-SceI nuclease in Drosophila

   Depositor

   Klaus Foerstemann

   Article

   Bottcher et al Nucleic Acids Res. 2014 Apr 19.

   Insert

   I-SceI (SCEI Budding Yeast)
   

   Use

   Tags

   HA and NLS

   Expression

   Insect

   Mutation

   Promoter

   tubulin

   Available since

   April 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSNA

   Plasmid

   #135192

   Purpose

   Arabinose inducible expression of lamba-Red recombinase proteins and I-SceI. Contains ampicillin resistance cassette

   Depositor

   Hongqing Fang

   Article

   Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d

   Insert

   I-SceI lamba Red
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   araBAD

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nano-lantern(cAMP-3.3)/pcDNA3

   Plasmid

   #53595

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Tol2-ngn1-Trpa1b-2A-mCherry

   Plasmid

   #106425

   Purpose

   For expressing Trpa1b-2A-mCherry in zebrafish Rohon-Beard neurons.

   Depositor

   Randall Peterson

   Article

   Lam et al Sci Rep. 2017 Sep 19;7(1):11839. doi: 10

   Inserts

   cmlc2 promoter
   Trpa1b (trpa1b Zebrafish)
   

   Use

   Gateway

   Tags

   2A-mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pegRNA_LMNA-1

   Plasmid

   #199273

   Purpose

   pegRNA used to induce LMNA (c.1824C > T) mutation

   Depositor

   Ting Zhou

   Article

   Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

   Insert

   LMNA 1824CtoT pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. Nano-lantern(cAMP-0.4)/pcDNA3

   Plasmid

   #53593

   Purpose

   Nano-lantern-based luminescent cAMP indicator

   Depositor

   Takeharu Nagai

   Article

   Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

   Insert

   Nano-lantern-based luminescent cAMP indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

8. sgRNA3_GAL4UAS-Luciferase reporter

   Plasmid

   #64159

   Purpose

   Photoactivatable transcription system. Lentiviral expression of sgRNA3 to target GAL4UAS-luciferase. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3 for GAL4UAS-Luciferase reporter
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEarleyGate103 mmPing20F

   Plasmid

   #140007

   Purpose

   mmPing20F activation tag

   Depositor

   Charles Nathan Hancock

   Article

   Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

   Insert

   mmPing20F
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLX_313-EGFP

    Plasmid

    #158580

    Purpose

    Lentiviral expression of EGFP

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. UBQ10:sXVE:(MCS)-3xHA-S11

    Plasmid

    #108180

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. UBQ10:sXVE:S10-(MCS)-3xHA

    Plasmid

    #108178

    Purpose

    Binary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    UBQ10:sXVE:

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pM423

    Plasmid

    #172408

    Purpose

    Expresses sfGFP (1-10) and MCP-GFP11

    Depositor

    Hajin Kim

    Article

    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

    Insert

    MCP, bipartite sfGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP {1-10}

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Sept. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSW002-PpsbA-E2-Crimson

    Plasmid

    #111258

    Purpose

    Broad host-range bacterial expression vector with constitutive PsbA promoter; for tagging bacteria with E2-Crimson

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    E2-Crimson
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PpsbA

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. P16ΔS:GFP1–9

    Plasmid

    #108186

    Purpose

    Binary vectors used for the constitutive expression of tripartite split-sfGFP components (GFP1-9) in plants

    Depositor

    Tzu-Yin Liu

    Article

    Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

    Insert

    GFP1-9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    P16ΔS

    Available since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-GBP1-10gly-lexADBD

    Plasmid

    #50795

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-lexA DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-puro-PARG shRNA

    Plasmid

    #23260

    Depositor

    Robert Sobol

    Article

    Tang et al Mol Cancer Res. 2010 Jan 12. ():.

    Insert

    poly(ADP-ribose) glycohydrolase shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pDEST-Cas9-tdTomato

    Plasmid

    #141013

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDV05.1_pEF1a-iRFP720_frameshifted

    Plasmid

    #200408

    Purpose

    Expresses a mutant iRFP720 mRNA constitutively in mammalian cells. A frameshift deletion in the ORF (compared to pDV05.0) prevents the correct translation of the fluorescent protein

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    iRFP720 (frameshift mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBS-KS-attB1-2-PT-SA-SD-2-2xTY1-V5

    Plasmid

    #61257

    Purpose

    CRISPR-RMCE, second step attB plasmid, intron phase 2, exchange dsRed with 2xTY1 and V5 tags cassette

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    2xTY1-V5
    

    Use

    CRISPR

    Tags

    TY1 and V5

    Expression

    Mutation

    Promoter

    Available since

    Nov. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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21. MT3

    Plasmid

    #105710

    Purpose

    Bacterial expression plasmids for production of recombinant metallothionein 3

    Depositor

    Artur Krężel

    Article

    Peris-Diaz et al J Proteome Res. 2021 Jan 1;20(1):776-785

    Insert

    Metallothionein 3 (MT3 Human)
    

    Use

    Tags

    Chiting Binding Domanin-Intein

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBT271.359

    Plasmid

    #127845

    Purpose

    clone A3A CBEs by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    A3A
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGG-cTP-TurboID

    Plasmid

    #209394

    Purpose

    Transiently expressing TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    cTP-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGG-NES-TurboID

    Plasmid

    #209393

    Purpose

    Transiently expressing TurboID fused with a nuclear export signal (NES) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    NES-3XHA-TurboID
    

    Use

    Tags

    Expression

    Plant

    Mutation

    TurboID gene sequence C249A

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMH4

    Plasmid

    #52529

    Purpose

    serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    2x FLAG-tag
    

    Use

    Blunt cloning kit

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. JL202: pMVP/mCherry-DEST

    Plasmid

    #121853

    Purpose

    pMVP destination vector, empty expression vector backbone w/ mCherry selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pBT138b-pro1

    Plasmid

    #138513

    Purpose

    P3h

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    T7-RNAP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJK503

    Plasmid

    #155380

    Purpose

    dCas12a oscillator, same direction crRNAs. dCas12a (D917A) + PA4-mVenus

    Depositor

    Pamela Silver

    Article

    Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

    Inserts

    dCas12a (F. novicida)
    PA4-mVenus
    dCas12a oscillator crRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    D917A (nuclease-deactivating)

    Promoter

    Available since

    May 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. JL002: pMVP/eGFP-DEST

    Plasmid

    #121852

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLN342 (FuGW-SSX1p-mKate2)

    Plasmid

    #105172

    Purpose

    SSX1 promoter drives mKate2 expression

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    SSX1p-mKate2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. YFP B1 in pcDNA3.1

    Plasmid

    #36397

    Depositor

    Narasimhan Gautam

    Article

    Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

    Insert

    G protein B1 (GNB1 Bovine)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

32. JC001: pMVP/Ad/RGD-DEST

    Plasmid

    #121844

    Purpose

    pMVP destination vector, empty RGD fiber-modified adenovirus vector backbone

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Adenoviral; Pmvp gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. P2A-mCherry-EhT backbone

    Plasmid

    #215549

    Purpose

    An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

    Depositor

    Kai Wang

    Article

    Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFSW FLEx mCherry-KASH

    Plasmid

    #139652

    Purpose

    Conditional expression of mCherry-KASH

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    mCherry-KASH
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Lsg-LRRK2-1

    Plasmid

    #199277

    Purpose

    nicking sgRNA to induce LRRK2 (c. 6055 G > A) mutation using PE3

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LRRK2 nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pegRNA_LRRK2-3

    Plasmid

    #199272

    Purpose

    pegRNA used to induce LRRK2 (c. 6055 G > A) mutation

    Depositor

    Ting Zhou

    Article

    Li et al Nat Commun. 2022 Oct 27;13(1):6354. doi:

    Insert

    LRRK2 6055GtoA pegRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTH

    Plasmid

    #56659

    Purpose

    Dual-expression vector for P. tricornutum

    Depositor

    Philip Weyman

    Article

    Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

    Insert

    KanR
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUC18T-mini-Tn7T-Gm-Pc-DsRed-Express2

    Plasmid

    #111260

    Purpose

    mini-Tn7 vector for insertion of DsRed-Express2 at bacterial attTn7 site

    Depositor

    Rosemarie Wilton

    Article

    Wilton et al Front Plant Sci. 2018 Feb 1;8:2242. doi:

    Insert

    DsRed-Express2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Pc

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-Tag 3A/WT myc-M1

    Plasmid

    #87719

    Purpose

    Expresses WT myc-M1 spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M1 isoform

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. KN206: pMVP/eCFP-DEST

    Plasmid

    #121854

    Purpose

    pMVP destination vector, empty expression vector backbone w/ eCFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Pmvp gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge