We narrowed to 5,565 results for: SPI

1. 186_pETcon_SARS2_XBB1-5

   Plasmid

   #208086

   Purpose

   yeast surface display of the SARS-CoV-2 XBB.1.5 variant RBD

   Depositor

   Tyler Starr

   Article

   Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

   Insert

   SARS-CoV-2 XBB.1.5 RBD (S Budding Yeast, SARS-CoV-2 virus)
   

   Use

   Tags

   HA and c-Myc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. hIgG1-Ty1-hFc

   Plasmid

   #194593

   Purpose

   Mammalian expression of Ty1 nanobody upstream of human IgG1

   Depositor

   Gerald McInerney

   Article

   Hanke et al Nat Commun. 2020 Sep 4;11(1):4420. doi:

   Insert

   Ty1-hFc (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-dLbCpf1-BE-YE2

   Plasmid

   #154146

   Purpose

   Expresses dLbCpf1-BE-YE2(dLbCas12a-BE-YE2) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YE2 (dCas12a-BE-YE2)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R132E), dLbCpf1(D832A+E925A+D1148A)

   Promoter

   CMV

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-dLbCpf1-BE-YEE

   Plasmid

   #154148

   Purpose

   Expresses dLbCpf1-BE-YEE(dLbCas12a-BE-YEE) in mammalian cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Commun. 2020 Aug 13;11(1):4072. doi:

   Insert

   dLbCpf1-BE-YEE (dCas12a-BE-YEE)
   

   Use

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   APOBEC1(W90Y+R126E+R132E), dLbCpf1(D832A+E925A+D1…

   Promoter

   CMV

   Available since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBBK48 pHomL-RPL18Bp-SPC42-Link-Neon(gfp)-SSA1t-HomR (AmpR)

   Plasmid

   #179032

   Purpose

   Vector containing a yeast integration cassette for RPL18B-driven expression of SPC42-Link-Neon(gfp), a marker for the spindle pole

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   RPL18Bp-SPC42-Link-Neon(gfp)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBbB8k-csg-NbSARS

   Plasmid

   #171703

   Purpose

   Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA is fused to a cross-reactive nanobody targeting the spike protein of betacoronaviruses via a flexible linker.

   Depositor

   Neel Joshi

   Article

   Birnbaum et al ACS ES T Water. 2022 Nov 11;2(11):1836-1

   Insert

   csgBACEFG, with nanobody fused to CsgA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUPD2 LbCas12a (GB1439)

   Plasmid

   #160573

   Purpose

   Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

   Insert

   LbCas12a
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pRPL28exon1-(Nhe/Mfe) SpHIS5 (pNTI619)

   Plasmid

   #115432

   Purpose

   Vector for expressing RPL28 with mutagenized exon2

   Depositor

   Nicholas Ingolia

   Article

   McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

   Inserts

   RPL28 (RPL28 Budding Yeast)
   HIS5
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   exon 2 deleted, with MfeI / NheI cloning site

   Promoter

   Ashbya gospii TEF1 and endogenous

   Available since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHH0103_NCKIPSD-1/1

   Plasmid

   #91376

   Purpose

   Protein expression and purification of human SH3 domain construct NCKIPSD-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   NCKIPSD-1/1 (NCKIPSD Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KHBD00189

    Plasmid

    #34341

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG2988 (ems Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

11. p5E-3x125bp-mnx1

    Plasmid

    #169043

    Purpose

    5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    Mnx1 enhancer (3x) (mnx1 Zebrafish)
    

    Use

    Gateway entry cloning vector

    Tags

    Expression

    Mutation

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX-2T-GST-eIF4E K119A

    Plasmid

    #112818

    Purpose

    Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Eif4e (Eif4e Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K119 mutated to A, increasing affinity for cap st…

    Promoter

    T7

    Available since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. HSV-DYN-hM4Di

    Plasmid

    #53327

    Purpose

    Dynorphin promoter driving hM4Di

    Depositor

    John Neumaier

    Article

    Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

    Insert

    hM4Di (Pdyn Rat)
    

    Use

    Hsv

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Rat dynorphin promoter ( -1858 to +135)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTE2690

    Plasmid

    #137717

    Purpose

    Expresses dimpLbCas12a-SunTag in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dimpLbCas12a
    

    Use

    CRISPR

    Tags

    NLSx3 SV40 and SunTagx10

    Expression

    Mammalian

    Mutation

    D156R, G532R, K538V, Y542R, K595R, D832A,

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3-LYRa11-RBD-8h

    Plasmid

    #161822

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate LYRa11

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T324-D522)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-SARS-1-RBD-8h

    Plasmid

    #161821

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-1 (Urbani strain)

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-RsSHC014-RBD-8h

    Plasmid

    #161826

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate RsSHC014

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET30a-SARS-CoV2-NP-Nt(47-173)

    Plasmid

    #165091

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pcDNA3-Rs7327-RBD-8h

    Plasmid

    #161823

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs7327

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTE3646

    Plasmid

    #137714

    Purpose

    Expresses LbCas12a RVRR variant in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    LbCas12a-RVRR
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pcDNA3-Rs4084-RBD-8h

    Plasmid

    #161825

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4084

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T321-D519)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pcDNA3-Rs4231-RBD-8h

    Plasmid

    #161824

    Purpose

    Mammalian expression plasmid for RBD of bat coronavirus isolate Rs4231

    Depositor

    Erik Procko

    Article

    Chan et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabf

    Insert

    Receptor-binding domain (RBD) of spike protein S
    

    Use

    Tags

    GSG linker and 8his tag and HA leader sequence

    Expression

    Mammalian

    Mutation

    Human codon optimized RBD (a.a. T320-D518)

    Promoter

    CMV

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET30a-SARS-CoV2-ORF3b (S23Q)

    Plasmid

    #165088

    Purpose

    protein expression in E. coli

    Depositor

    Rick Tarleton

    Article

    Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

    Insert

    Severe acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Stop codon to glutamine at 23rd amino acid

    Promoter

    Available since

    Feb. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. DF-UAS-sspa-GCaMP6f

    Plasmid

    #67569

    Purpose

    Zebrafish expression of a short photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    sspa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pFUW-tetO-Irf4

    Plasmid

    #139804

    Purpose

    doxycycline-inducible expression of mouse Irf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Irf4 (Irf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTE3987

    Plasmid

    #137716

    Purpose

    Expresses dLbCas12a-RVRR-BE in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2020 Feb 28. pii: 576

    Insert

    dLbCas12a-RVRR-BE
    

    Use

    Tags

    6xHis, APOBEC-1, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    G532A, K538V, Y542R, K595R

    Promoter

    CMV

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. DF-UAS-spa-GCaMP6f

    Plasmid

    #67568

    Purpose

    Zebrafish expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    spa-GCaMP6f
    

    Use

    Zebrafish transgenesis (see :a spinal opsin contr…

    Tags

    HIS-tag

    Expression

    Mutation

    K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

    Promoter

    Available since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pSFG-TFII-I-GFP Y248&249F

    Plasmid

    #22196

    Depositor

    Ananda Roy

    Article

    Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Tyrosines 248 and 249 to Phenylalanines. …

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

29. pUC19-Q0P897

    Plasmid

    #103120

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    Q0P897(Cas9 coding gene from Rhodospirillum rubrum (strain ATCC 11170 / NCIB 8255))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUC19-D3NT09

    Plasmid

    #103074

    Purpose

    Cas9 coding gene template with optimized sequence for human codon usage

    Depositor

    Duhee Bang

    Article

    Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

    Insert

    D3NT09(Cas9 coding gene from Azospirillum sp. (strain B510))
    

    Use

    CRISPR; Cloning vector

    Tags

    Expression

    Mutation

    human codon-optimized

    Promoter

    Available since

    April 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pABE8e-NG-403Q-Nterm-AAV

    Plasmid

    #194651

    Purpose

    N-terminal AAV genome plasmid encoding ABE8e + sgRNA to correct the R403Q mutation in mice

    Depositor

    David Liu

    Article

    Reichart et al Nat Med. 2023 Feb;29(2):412-421. doi: 10

    Insert

    ABE8e-NG N-term
    

    Use

    AAV

    Tags

    BPNLS and NpuN spilt intein

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pSEVA228-pro4IUPi

    Plasmid

    #122018

    Purpose

    Expresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.

    Depositor

    Gregory Stephanopoulos

    Article

    Chatzivasileiou et al Proc Natl Acad Sci U S A. 2018 Dec 24. p

    Inserts

    Choline kinase (CKI1 Budding Yeast)
    Isopentenyl monophosphate kinase (AT1G26640 Mustard Weed)
    isopentenyl-diphosphate Delta-isomerase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pro4

    Available since

    Oct. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNK5712

    Plasmid

    #219752

    Purpose

    pGAP-like vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - nnH3H_v2 - tAOX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pFPV25-cR-2iG

    Plasmid

    #204618

    Purpose

    Fluorescent reporter for SPI2 induction in Salmonella

    Depositor

    Jacques Neefjes

    Article

    Stévenin et al Cell Rep. 2024 Nov 1;43(11):114931. doi:

    Inserts

    rpsM promoter
    sseA promoter
    

    Use

    Tags

    GFP and dsRed

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNK5867

    Plasmid

    #219751

    Purpose

    pGAP-like vector encoding hispidin-synthase from Mycena citricolor under control of GAP promoter, for yeast expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pGAP - mcitHispS - tAOX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDEST27-GST-hMSN

    Plasmid

    #211823

    Purpose

    Express human MSN in mammalian cells

    Depositor

    Haian Fu

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Use

    Tags

    GST tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pX028

    Plasmid

    #167151

    Purpose

    MoClo-compatible Level 1 (position 2) vector encoding Neonothopanus nambi hispidin synthase nnHispS under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnHispS-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX029

    Plasmid

    #167152

    Purpose

    MoClo-compatible Level 1 (position 3) vector encoding Neonothopanus nambi hispidin-3-hydroxylase nnH3H under control of 0.4 kb 35S promoter, for expression in plants

    Depositor

    Karen Sarkisyan

    Article

    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

    Insert

    p35s-nnH3H-ocsT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTE4561

    Plasmid

    #107525

    Purpose

    Expresses Lb crRNA and mCherry in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pTE3320

    Plasmid

    #107529

    Purpose

    Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Insert

    hLbCpf1(RVR)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    G532R, K538V, Y542R

    Promoter

    CMV

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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42. pMBS-852

    Plasmid

    #218010

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pMBS-850

    Plasmid

    #218005

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pMBS-857

    Plasmid

    #218007

    Purpose

    Receptor binding domain of SARS-CoV-2

    Depositor

    Michael Schroda

    Article

    Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

    Insert

    RBD (S SARS-CoV-2)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pNK291

    Plasmid

    #219748

    Purpose

    DVK_AF CIDAR vector encoding mutant of Neonothopanus nambi hispidin-3-hydroxylase nnH3H_v2 under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - nnH3H_v2 - tSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pOPINVHH_H6_His

    Plasmid

    #201719

    Purpose

    Expression of nanobody

    Depositor

    Ray Owens

    Article

    Cornish et al Open Biol. 2024 Jun;14(6):230252. doi: 1

    Insert

    VHH_H6 (S )
    

    Use

    Tags

    Hexahistidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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47. pIG-728pUC19-tNGFR-P2A-WIP

    Plasmid

    #186094

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    WIP (WIPF1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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48. pMIG-IRF4

    Plasmid

    #58987

    Purpose

    MSCV based expression vector - co-expresses GFP and murine IRF4

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Insert

    Mouse IRF4 (Irf4 Mouse)
    

    Use

    Retroviral

    Tags

    Kozak sequence added

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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49. pInducer20 ALPK1 V1092A

    Plasmid

    #231588

    Purpose

    ALPK1 gene mutated in position V1092A (mutation found in spiradenomacarcinoma (sweat gland cancer)) under control of a doxycycline-inducible promoter

    Depositor

    Thomas Henry

    Article

    Martin et al iScience. 2024 Dec 10;28(1):111563. doi:

    Insert

    ALPK1 (ALPK1 Human)
    

    Use

    Lentiviral

    Tags

    3X flag

    Expression

    Mammalian

    Mutation

    changed Valine1092 to Alanine

    Promoter

    doxycycline

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. TFORF0863

    Plasmid

    #143732

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GTF2I (GTF2I Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge