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Flag-FOXS1

Plasmid

#153152

Purpose

Expression of Flag tagged human FOXS1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXS1 (FOXS1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pUPD2

Plasmid

#68161

Purpose

Universal domesticator plasmid for level 0 parts

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology; Domestication of dna parts for…

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGIPZ EF1a IRES mNeonGreen-P2A-Puro

Plasmid

#248030

Purpose

Backbone that constitutively expresses mNeonGreen and a puromycin resistance gene in human cells

Depositor

Joshua Leonard

Article

Deol et al GEN Biotechnol. 2023 Jun 1;2(3):228-246.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2026

Availability

Academic Institutions and Nonprofits only

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tagSrc-HaloTag

Plasmid

#179141

Purpose

The tag is SsrA (AANDENY). Tag is inserted after N534 of Src.

Depositor

Klaus Hahn

Article

Liu et al Cell. 2021 Oct 28;184(22):5670-5685.e23.

Insert

Src, SsrA

Tags

HaloTag

Expression

Mammalian

Available Since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP HA

Plasmid

#59759

Purpose

encodes multiple copies of an epitope tag HA

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _HA

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

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Flag-FOXC2

Plasmid

#153115

Purpose

Expression of Flag tagged human FOXC2 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXC2 (FOXC2 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX.TRE3G.AdProt

Plasmid

#131687

Purpose

A lentiviral vector for tTA-regulated expression of the adenovirus protease using the tetracycline transactivator transcription factor.

Depositor

Matthew D. Shoulders

Article

Berman et al J Am Chem Soc. 2018 Dec 26;140(51):18093

Insert

Adenovirus Protease

Use

Lentiviral

Promoter

TRE3GV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-TRIM28

Plasmid

#124960

Purpose

Mammalian expression of Human TRIM28

Depositor

Michelle Barton

Article

Li et al Oncogene. 2017 May 25;36(21):2991-3001.

Insert

hTRIM28 (TRIM28 Human)

Tags

FLAG

Expression

Mammalian

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pIVT-iCre

Plasmid

#232773

Purpose

IVT template for making modRNA encoding iCre

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

iCre

Use

Cre/Lox; In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt3

Plasmid

#184504

Purpose

Mammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 3 (Syt3 Rat)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pPnos (GB0072)

Plasmid

#68166

Purpose

Provides the Agrobacterium tumefaciens Nopaline synthase promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Nos promoter

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCEP4-AD8gp160

Plasmid

#123260

Purpose

Mammalian expression plasmid for Env from the AD8 HIV-1 isolate

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 (AD8) Env

Tags

CD5 leader peptide

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available Since

April 1, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP2

Plasmid

#42308

Depositor

Thierry Galli

Article

Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

Insert

VAMP 2

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only

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pDGB3_alpha1

Plasmid

#68228

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Expression

Plant

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
CMV-mito-Citroff1-S245

Plasmid

#134306

Purpose

mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

Depositor

Robert Campbell

Article

Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

Insert

Citroff1 S245A

Expression

Mammalian

Promoter

CMV

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

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MCP/PCP RNA Lantern

Plasmid

#226095

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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hE-cadherin-pcDNA3

Plasmid

#45769

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

hE-cadherin (CDH1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

June 24, 2013

Availability

Academic Institutions and Nonprofits only

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P74-26 Tail Tube Protein (gp93) in pSMT3

Plasmid

#191261

Purpose

Expresses P74-26 gp93 with a His-SUMO tag in bacterial cells

Depositor

Brian Kelch

Article

Agnello et al J Biol Chem. 2023 Feb 13:103021. doi: 10

Insert

P74-26 gp93

Tags

His-SUMO

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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TL022_lenti_hSyn:SEP-GluA1

Plasmid

#192516

Purpose

Expression of AMPA GluA1 with N terminal super ecliptic phluorin (SEP) fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

SEP-GluA1

Use

Lentiviral

Tags

super ecliptic phluorin

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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CL7-LbCas12a

Plasmid

#164658

Purpose

Bacterial expression of LbCas12a using CL7/Im7

Depositor

Piyush Jain

Article

Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

Insert

LbCas12a

Tags

Thioredoxin, CL7, SUMO

Expression

Bacterial

Promoter

T7

Available Since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-miniGFP2-P2A-FusionRed

Plasmid

#188734

Purpose

Express miniGFP2 and FusionRed in mammalian cells

Depositor

Kiryl Piatkevich

Article

Liang et al Front Bioeng Biotechnol. 2022 Oct 17;10:

Insert

miniGFP2

Use

AAV

Tags

FusionRed

Expression

Mammalian

Promoter

CAG

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-mTagBFP

Plasmid

#75175

Purpose

TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

TagBFP

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCEP4-SERT-myc

Plasmid

#107456

Purpose

Mammalian expression plasmid for codon optimized hSERT with internal MYC tag inserted in ECL2

Depositor

Erik Procko

Article

Young et al bioRxiv 2021.04.19.440442

Insert

Serotonin Transporter (SERT) (SLC6A4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pIVT-PuroR

Plasmid

#232753

Purpose

IVT template for making modRNA encoding the Puromycin resistance gene

Depositor

Kate Galloway

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

PuroR

Use

In vitro transcription

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI pHluorin Syt4

Plasmid

#184505

Purpose

Mammalian expression of Synaptotagmin 4 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 4 (Syt4 Rat)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDG04439

Plasmid

#216365

Purpose

xhNup133-Nb2t (+2Cys) Nanobody production

Depositor

Dirk Görlich

Article

Sola Colom et al EMBO J. 2024 Jun;43(11):2198-2232. doi:

Insert

xhNup133-Nb2t (+2Cys)

Use

Affinity Reagent/ Antibody

Tags

14xHis-NEDD8

Expression

Bacterial

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pBECAb-apr

Plasmid

#122001

Purpose

A cytidine deaminase-mediated base-editing plasmid in Acinetobacter baumannii

Depositor

Quanjiang Ji

Article

Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

Type

Empty backbone

Use

CRISPR

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP/PCP yellow RNA Lantern

Plasmid

#226096

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-Venus d12-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
MCP/PCP red RNA Lantern

Plasmid

#226097

Purpose

RNA tracking

Depositor

Jennifer Prescher

Article

Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

Insert

NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA_CAG-EGFP

Plasmid

#229799

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA Donor_hEF1a

Plasmid

#229779

Purpose

Bxb1-GA donor plasmid for the cloning of payloads <20 kb with hEF1a promoter for expressing landing pad enrichment marker

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pET29b-dspB(E184Q)-6xHis

Plasmid

#175802

Purpose

Plasmid for overexpression of recombinant His-tagged protein DspB(E184Q), used as a probe for binding to biofilm polysaccharide PNAG.

Depositor

Mark Nitz

Article

Eddenden et al ACS Chem Biol. 2020 May 15;15(5):1204-12

Insert

Dispersin B

Tags

Hexahistidine tag

Expression

Bacterial

Mutation

E184Q

Promoter

T7

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pGMC00029 (aka pRC0610)

Plasmid

#200834

Purpose

Lentiviral guide RNA expression plasmid for CRISPR activation with puromycin/BFP

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_smFP FLAG

Plasmid

#59756

Purpose

encodes multiple copies of an epitope tag FLAG

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _FLAG

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 21, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TL028_AAV_hSyn:HaloTag-GluA1

Plasmid

#192520

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

AAV and Cre/Lox

Tags

HaloTag

Promoter

hSyn

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA-CMV-mStayGold

Plasmid

#241383

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CMV promoter

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

mStayGold

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 15, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-kdrl

Plasmid

#78687

Purpose

kdrl promoter for Gateway cloning

Depositor

Jau-Nian Chen, Sarah Childs

Article

Choi et al Dev Biol. 2007 Apr 15;304(2):735-44. Epu

Insert

kdrl promoter (kdrl Zebrafish)

Use

Unspecified

Promoter

kdrl

Available Since

June 5, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
IKKε v2-V5

Plasmid

#169770

Purpose

Express IKKε v2 in mammalian cells

Depositor

Sung-Liang Yu

Article

Chang et al Commun Biol. 2021 Jun 2;4(1):663. doi: 1

Insert

Homo sapiens inhibitor of nuclear factor kappa B kinase subunit epsilon (IKBKE), transcript variant 2

Tags

V5 tag

Expression

Bacterial and Mammalian

Available Since

July 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW-LIC-N8C_Fab3b

Plasmid

#203685

Purpose

Expresses N8C_Fab3b

Depositor

Brenda Schulman

Article

Henneberg et al Nat Chem Biol. 2023 Dec;19(12):1513-1523

Insert

N8C_Fab3b

Expression

Bacterial

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-Mem

Plasmid

#55779

Purpose

This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mCherry.

Depositor

Catherine Berlot

Article

Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

Insert

mCherry-Mem

Tags

The N-terminal 20 residues of neuromodulin are fu…

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
hSERT-N1-pEYFP

Plasmid

#70105

Purpose

mammalian expression of hSERT with C-terminal YFP fusion

Depositor

Harald Sitte

Article

Just et al J Biol Chem. 2004 Feb 20;279(8):6650-7.

Insert

SERT (SLC6A4 Human)

Tags

EYFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pRAM28

Plasmid

#234314

Purpose

pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

Depositor

James Chappell

Article

Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

Insert

U1367 Targeting RAM, Barcode 2

Use

Synthetic Biology

Promoter

P. CymRC

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRISPR_eGFP_497

Plasmid

#111824

Purpose

Knockout eGFP

Depositor

Steve Shih

Article

Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

Insert

gRNA targeting eGFP 477-497

Use

CRISPR

Tags

mCherry

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge

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