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pLVX-CMV-Galpha-i2 ONE-GO

Plasmid

#189736

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i2 ONE-GO
GNAI2 (Gnai2 Rat)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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μ2-HA-WT

Plasmid

#32752

Depositor

Alexander Sorkin

Article

Nesterov et al EMBO J. 1999 May 4;18(9):2489-99.

Insert

μ2 subunit of clathrin adaptor complex AP-2 (Ap2m1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2011

Availability

Academic Institutions and Nonprofits only

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pRK7-HA-S6K1-E389-deltaCT

Plasmid

#8993

Depositor

John Blenis

Article

Schalm et al Curr Biol. 2002 Apr 16. 12(8):632-9.

Insert

S6K1 (Rps6kb1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T389E, stop codon at amino acid 402

Promoter

Available since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Barr2 flag

Plasmid

#14685

Depositor

Robert Lefkowitz

Article

Laporte et al Proc Natl Acad Sci U S A. 1999 Mar 30. 9

Insert

beta-arrestin 2 (Arrb2 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
Kif5A-CIBN

Plasmid

#102251

Purpose

Expresses fusion of kinesin heavy chain 5A (1-572) and CIB1 (1-170)

Depositor

Bianxiao Cui

Article

Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

Insert

Kif5A-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D rM3D mCherry SV40

Plasmid

#92285

Purpose

AAV DREADD expression

Depositor

Baljit Khakh

Article

Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

Insert

rM3D

Use

AAV

Tags

mCherry

Expression

Mutation

Promoter

GfaABC1D

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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alpha1-EGFP

Plasmid

#118952

Purpose

GABAA receptor expression (alpha1 subunit). EGFP tag at the C-terminus.

Depositor

Angel de Blas

Article

Christie et al Mol Cell Neurosci. 2006 Jan;31(1):1-14.

Insert

GABRA1 (Gabra1 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LAMP1-SBP-GFP

Plasmid

#120172

Purpose

Expresses a chimera of LAMP1, SBP tag and GFP (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

Lysosomal-associated membrane protein 1 (Lamp1 Rat)

Use

Tags

SBP and eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBiFC-bJunVN173

Plasmid

#22012

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

bJunVN173 (Jun Rat, Aequorea Victoria)

Use

Tags

FLAG

Expression

Mammalian

Mutation

c-Jun(257-338)-Venus(1-172)

Promoter

Available since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pGEX-T-rnVAPA-1-219

Plasmid

#13395

Depositor

Axel Brunger

Article

Kaiser et al Structure. 2005 Jul . 13(7):1035-45.

Insert

VAP-A (Vapa Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2009

Availability

Academic Institutions and Nonprofits only
pAS_4xhyb PylT A41AA C55A CRFR1 95TAG 263TAA-HA

Plasmid

#154781

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAG 263TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

CRFR1 (Crhr1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

95TAG 263TAA in CRFR1-HA, hybrid PylT with A41AA …

Promoter

EF1

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

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miniCGBE1 (pBM1219)

Plasmid

#140253

Purpose

CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

Depositor

Keith Joung

Article

Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

Insert

BE4max(R33A)-ΔUGI

Use

Tags

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in Cas9

Promoter

CMV

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-hSyn-Galpha-oA ONE-GO

Plasmid

#204335

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Insert

hSyn Galpha-oA ONE-GO

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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KIF5C(1-560)-2xmCherry-EF(C)

Plasmid

#61664

Purpose

Tandem mCherry-labeled, truncated kinesin-1 fused to EF Hand linker

Depositor

Kristen Verhey

Article

Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

Insert

KIF5C(1-560)-2xmCherry-EF(C) (Kif5c Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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HSV-DYN-hM4Di

Plasmid

#53327

Purpose

Dynorphin promoter driving hM4Di

Depositor

John Neumaier

Article

Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

Insert

hM4Di (Pdyn Rat)

Use

Hsv

Tags

3xHA tag

Expression

Mammalian

Mutation

Promoter

Rat dynorphin promoter ( -1858 to +135)

Available since

May 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

Beta-actin

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

Plasmid

#174900

Purpose

amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

Insert

CRFR1 (Crhr1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

A95TAG

Promoter

EF1

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK7 T194D

Plasmid

#80886

Purpose

mammalian expression of ALK7 T194D

Depositor

Aristidis Moustakas

Article

Moren et al Oncogene. 2000 Sep 7;19(38):4396-404.

Insert

ALK7 (Acvr1c Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

T194D (constitutively active)

Promoter

CMV

Available since

Aug. 25, 2016

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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K44A dynamin 2 pEGFP

Plasmid

#34687

Depositor

Sandra Schmid

Insert

dynamin 2 (Dnm2 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

K44A

Promoter

Available since

Feb. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pORANGE Dlg4-GFP KI

Plasmid

#131477

Purpose

Endogenous tagging of PSD95: C-terminal (amino acid position: R721)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Dlg4, Dlg4 Rat, Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6 and Cbh

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-CREB

Plasmid

#137001

Purpose

mEGFP-CREB donor for G-CREB

Depositor

Ryohei Yasuda

Article

Laviv et al Neuron. 2019 Dec 17. pii: S0896-6273(19)

Insert

CREB1 (Creb1 Rat)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

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pMMTV neu NT

Plasmid

#1823

Depositor

Philip Leder

Article

Muller et al Cell 1988 Jul 1;54(1):105-15.

Insert

neu NT (Erbb2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
TrkC-GFP

Plasmid

#24094

Depositor

Moses Chao

Insert

TrkC (Ntrk3 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2013

Availability

Academic Institutions and Nonprofits only
pET42b-HF-BE3

Plasmid

#87438

Purpose

Expresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expression

Depositor

David Liu

Article

Rees et al Nat Commun. 2017 Jun 6;8:15790. doi: 10.

Insert

HF-BE3 (Apobec1 Bacillus phage PBS2, Rat, Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

June 13, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII

Plasmid

#165431

Purpose

Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII

Use

Tags

tdTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
ankG-mCherry

Plasmid

#42566

Depositor

Benedicte Dargent

Article

Leterrier et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

ankyrin G (Ank3 Rat)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 26, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-Pak1 83-149

Plasmid

#12216

Depositor

Jonathan Chernoff

Article

Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

Insert

Pak1 (Pak1 Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Dominant negative Pak1: autoinhibitory domain, aa…

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only
pLenti-XI-Neo-GST- AMPK- Kinase Dead (K to R)

Plasmid

#139844

Purpose

Lentiviral expression of GST-tagged rat AMPK- Kinase Dead (K to R)

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

AMPK-Kinase-dead (Prkaa2 Rat)

Use

Lentiviral

Tags

GST

Expression

Mutation

K47R

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

Plasmid

#218862

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8m

Use

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

A25G F286Y

Promoter

hsp70

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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