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Plasmid#139818Purposedoxycycline-inducible expression of Human NR4A3 in mammalian cellsDepositorInsertNR4A3 (NR4A3 Human)
UseLentiviralTagsExpressionMammalianMutationS240G- please see depositor comment belowPromoterTRE-mCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHalo-MyosinIIB-C25
Plasmid#128574PurposeStudies of cellular contractility.DepositorInsertMyosin IIB (MYH10 Human)
UseTagsHaloExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pcDNA1 neo
Plasmid#13580DepositorInsertintegrin beta 6 (ITGB6 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig1
Plasmid#64841PurposeA lentiviral vector for the expression of mouse Olig1 under doxycyline controlDepositorInsertOlig1 (Olig1 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hcMYC (Plath)
Plasmid#17966DepositorInsertv-myc myelocytomatosis viral oncogene homolog (avian) (MYC Human)
UseRetroviralTagsExpressionMammalianMutationn/aPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
MoFGFR2b-ires neo
Plasmid#86491Purposemamalian cell expression of FGFR2bDepositorInsertFgfrR2 IIIb 13A4 (FGFR2 Human)
UseTagsExpressionMammalianMutationPromoterMoloney murine leukemia virus LTRAvailable sinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hKLF4 (Plath)
Plasmid#17967DepositorInsertKruppel-like factor 4 (gut) (KLF4 Human)
UseRetroviralTagsExpressionMammalianMutationN/APromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
HA-VHL Y98H-pRc/CMV
Plasmid#22044DepositorInsertvon Hippel-Lindau tumor suppressor (VHL) (VHL Human)
UseTagsHA-tagExpressionMammalianMutationpoint mutant, converting aa 98 from Tyrosine to H…PromoterAvailable sinceSept. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129E
Plasmid#36050DepositorInsertalpha-synuclein S129E (SNCA Human)
UseTagsExpressionBacterialMutationS129EPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129A
Plasmid#36048DepositorInsertalpha-synuclein S129A (SNCA Human)
UseTagsExpressionBacterialMutationS129APromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMXs-hSOX2 (Plath)
Plasmid#17965DepositorInsertSRY (sex determining region Y)-box 2 (SOX2 Human)
UseRetroviralTagsExpressionMammalianMutationN/APromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
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psiCHECK-2-TGFBR2-wt_3'UTR
Plasmid#31882DepositorInsertTGFBR2 wild-type 3'UTR (TGFBR2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterSV40Available sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-SLC35B2-sgRNA
Plasmid#154860PurposeLentiviral expression of Cas9 and sgRNA targeting SLC35B2DepositorInsertSLC35B2 sgRNA (SLC35B2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-GTSE1-His6
Plasmid#69109PurposeFor bacterial expression of recombinant human GTSE1 tagged with MBP and His.DepositorInsertG2- and S-phase Expressed 1 (GTSE1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT7-7 asyn S129G
Plasmid#36049DepositorInsertalpha-synuclein S129G (SNCA Human)
UseTagsExpressionBacterialMutationS129GPromoterT7Available sinceJuly 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-Myb
Plasmid#139826Purposedoxycycline-inducible expression of mouse Myb in mammalian cellsDepositorInsertMyb (Myb Mouse)
UseLentiviralTagsExpressionMammalianMutationP94H and Q619R- Please see depositor comment belo…PromoterTRE-mCMVAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-Lmo2
Plasmid#60666PurposePiggyBac expression vector encoding murine Lmo2 downstream of a doxycycline inducible promoterDepositorInsertLmo2 (Lmo2 Mouse)
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD/CMV/V5-DEST-Rac1-2G
Plasmid#66112PurposeSecond generation Rac1 FRET biosensor for adenovirus productionDepositorInsertSecond generation Rac1 FRET biosensor
UseAdenoviralTagsHis-MycExpressionMammalianMutationPromoterCMVAvailable sinceOct. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nanog-luciferase targeting construct
Plasmid#26591DepositorInsertNanog promoter-luciferase-lox-pgk-neomycin-loc
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pT7[mRNA]-5'UTR:mMyod1[NM_010866.2]: Hba-a1_3'UTR
Plasmid#205026PurposeIn vitro transcription vector for production of synthetic mRNA encoding for mouse Myod1DepositorInsertMyod1 (Myod1 Mouse)
UseSynthetic Biology; In vitro transcription vector …TagsExpressionMutationPromoterAvailable sinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXs-HOXA9
Plasmid#131608Purposeexpression of human HOXA9 in mammalian cellsDepositorInsertHOXA9 (HOXA9 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
α-actinin-4-M522-sstFRET(Venus/Cerulean) in pUNO1
Plasmid#190799PurposeImaging of force-sensitive α-actinin-4 in live cellsDepositorInsertα-actinin-4-Venus-Cerulean-522 (ACTN4 Human, Synthetic)
UseTagscerulean and venusExpressionMammalianMutationPromoterhEF1-HTLVAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4-hB90
Plasmid#18968DepositorInsertBrevican C-terminus (BCAN Human)
UseTagsExpressionMammalianMutationHuman brevican signal peptide aa 1-23 followed by…PromoterAvailable sinceDec. 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pLJM1-SLC35B2-FLAG
Plasmid#154863PurposeTransient or Lentiviral expression of sgRNA (Addgene#154860) resistant SLC35B2DepositorInsertSLC35B2 (SLC35B2 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationctcctggtgcagtacttc => ttattagtccaatattttPromoterCMVAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmKikGR Vinculin
Plasmid#105316PurposePhotoconvertable focal adhesion protein vinculin to study kinetics or dynamicsDepositorInsertvinculin (VCL Chicken)
UseTagsKikGRExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
FN(9-10)-I27-CC
Plasmid#85394PurposeMTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labelingDepositorInsertFibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
UseTags6x HisExpressionBacterialMutationPromoterT7Available sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Sox15
Plasmid#128831Purposedox-dependent expression of mouse Sox15DepositorInsertSox15 (Sox15 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
integrin alpha9 pBS
Plasmid#13584DepositorInsertintegrin alpha 9 (ITGA9 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Rhoc-m_3'UTR
Plasmid#31885DepositorInsertRhoc mutant 3'UTR (RHOC Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationmiR-302b, 372 7-mer seed match mutated from AGCAC…PromoterSV40Available sinceSept. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRCA905 - KLF4 Puro-T2A-BFP (pRCA847 backbone)
Plasmid#238185PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Human, Synthetic)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845 backbone)
Plasmid#238181PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Human, Synthetic)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA903 - KLF5 isoform 1 Puro-T2A-BFP (pRCA845 backbone)
Plasmid#238184PurposeLentiviral vector for overexpression KLF5 ORF from a EF1a promoterDepositorInsertKLF5 (KLF5 Human, Synthetic)
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA376 - pBA904 Puro-T2A-GFP NTC guide (pRCA360 backbone)
Plasmid#238167PurposeLentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct CaptureDepositorInsertNon-targeting sgRNA
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRCA881 - KLF4 Puro-T2A-GFP (pRCA847 backbone)
Plasmid#238182PurposeLentiviral vector for overexpression KLF4 ORF from a EF1a promoterDepositorInsertKLF4 (KLF4 Human, Synthetic)
UseLentiviralTagsGFPExpressionMutationPromoterAvailable sinceJune 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3D+3N
Plasmid#234614PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to asparagineDepositorInserthnRNPA1_LCD_3DN (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214N, D242N, D250NPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -4D+4N
Plasmid#234611PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to asparagineDepositorInserthnRNPA1_LCD_4DN (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214N, D242N, D250N, D262NPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -4D+4V
Plasmid#234610PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 4 aspartates mutated to valineDepositorInserthnRNPA1_LCD_4DV (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214V, D242V, D250V, D262VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
A1-LCD -3D+3V
Plasmid#234613PurposeBacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with 3 aspartates mutated to valineDepositorInserthnRNPA1_LCD_3DV (HNRNPA1 Human)
UseTags6xHis-TEVExpressionBacterialMutationD214V, D242V, D250VPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GCaMP6f-WPRE
Plasmid#216275PurposeAAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of green fluorescent calcium indicator GCaMP6fDepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterhuman Synapsin 1 (hSyn)Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-FAS-GCaMP6f-WPRE
Plasmid#141237PurposeAAV vector with Ef1a promoter and LoxFAS sites for Cre-Off expression of GCaMP6f (GCaMP6f will not be expressed in mammalian cells that express Cre recombinase)DepositorInsertGCaMP6f
UseAAV and Cre/LoxTags6XHIS and XpressExpressionMutationPromoterHuman elongation factor-1 alpha (EF-1 alpha)Available sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7 (2-180)
Plasmid#190066PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEVExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TEV-A7-Strep (2-180)
Plasmid#190067PurposeCodon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.DepositorInsertAnnexin A7 PRD (ANXA7 Human)
UseTagsGB1-His-TEV and StrepExpressionBacterialMutationPRD only, residues 2-180PromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-XYLT2-sgRNA
Plasmid#154862PurposeLentiviral expression of Cas9 and sgRNA targeting XYLT2DepositorInsertXYLT2 sgRNA (XYLT2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
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HOP-flash
Plasmid#83467PurposeTEAD-YAP/TAZ tx activity reporter. 8xwt TEAD binding sites with minimal promoter plus luciferase reporter geneDepositorInsertMultimerized TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
phDLX1-N174
Plasmid#60859Purpose2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoterDepositorInsertDLX1 (DLX1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only